Article: EZH2 supports ovarian carcinoma cell invasion and/or metastasis via regulation of TGF-β 1 and is a predictor of outcome in ovarian carcinoma patients
| Title | EZH2 supports ovarian carcinoma cell invasion and/or metastasis via regulation of TGF-β 1 and is a predictor of outcome in ovarian carcinoma patients | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Rao, ZY1 Cai, MY1 Yang, GF1 He, LR1 Mai, SJ1 Hua, WF1 Liao, YJ1 Deng, HX1 Chen, YC2 Guan, XY1 Zeng, YX1 Kung, HF1 2 Xie, D1 | ||||||
| Issue Date | 2010 | ||||||
| Publisher | Oxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/ | ||||||
| Citation | Carcinogenesis, 2010, v. 31 n. 9, p. 1576-1583 [How to Cite?] DOI: http://dx.doi.org/10.1093/carcin/bgq150 | ||||||
| Abstract | It was suggested that the enhancer of zeste homolog 2 (EZH2) gene is a putative candidate oncogene in several types of human cancer. The potential oncogenic role of EZH2 and its clinical/prognostic significance, however, in ovarian carcinoma are unclear. In this study, EZH2 expression was examined by immunohistochemistry (IHC) in cohorts of normal and tumorous ovarian tissues. High expression of EZH2 was examined in none of the normal ovaries, in 3% of the cystadenomas, in 23% of the borderline tumors and in 50% of the ovarian carcinomas, respectively. In the ovarian carcinomas, high expression of EZH2 was positively correlated with an ascending histological grade and/or advanced stage of the disease (P < 0.05). Moreover, high expression of EZH2 in ovarian carcinoma was determined to be a strong and an independent predictor of short overall survival (P < 0.05). In ovarian carcinoma HO-8910 and UACC-326 cell lines, EZH2 knockdown by RNA interference led to a G 1 phase cell cycle arrest, reduced cell growth/proliferation and inhibited cell migration and/or invasion in vitro. In addition, EZH2 knockdown was found to reduce transforming growth factor-β1 (TGF-β1) expression and increase E-cadherin expression either in the transcript or in the protein levels. Furthermore, a significant positive correlation between overexpression of EZH2 and TGF-β1 in ovarian carcinoma tissues was observed (P < 0.001). These findings suggest a potential important role of EZH2 in the control of cell migration and/or invasion via the regulation of TGF-β1 expression, and the high expression of EZH2, as detected by IHC, is an independent molecular marker for shortened survival time of patients with ovarian carcinoma. © The Author 2010. Published by Oxford University Press. All rights reserved. | ||||||
| ISSN | 0143-3334 2011 Impact Factor: 5.702 2011 SCImago Journal Rankings: 0.692 | ||||||
| DOI | http://dx.doi.org/10.1093/carcin/bgq150 | ||||||
| ISI Accession Number ID | WOS:000281530400010
Funding Information: Major State Basic Research Program of China (2006CB910104 and 2010CB529401); Nature Science Foundation of China (30772334). | ||||||
| References | References in Scopus |
| dc.contributor.author | Rao, ZY | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Cai, MY | ||||||
| dc.contributor.author | Yang, GF | ||||||
| dc.contributor.author | He, LR | ||||||
| dc.contributor.author | Mai, SJ | ||||||
| dc.contributor.author | Hua, WF | ||||||
| dc.contributor.author | Liao, YJ | ||||||
| dc.contributor.author | Deng, HX | ||||||
| dc.contributor.author | Chen, YC | ||||||
| dc.contributor.author | Guan, XY | ||||||
| dc.contributor.author | Zeng, YX | ||||||
| dc.contributor.author | Kung, HF | ||||||
| dc.contributor.author | Xie, D | ||||||
| dc.date.accessioned | 2012-06-26T06:11:40Z | ||||||
| dc.date.available | 2012-06-26T06:11:40Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | It was suggested that the enhancer of zeste homolog 2 (EZH2) gene is a putative candidate oncogene in several types of human cancer. The potential oncogenic role of EZH2 and its clinical/prognostic significance, however, in ovarian carcinoma are unclear. In this study, EZH2 expression was examined by immunohistochemistry (IHC) in cohorts of normal and tumorous ovarian tissues. High expression of EZH2 was examined in none of the normal ovaries, in 3% of the cystadenomas, in 23% of the borderline tumors and in 50% of the ovarian carcinomas, respectively. In the ovarian carcinomas, high expression of EZH2 was positively correlated with an ascending histological grade and/or advanced stage of the disease (P < 0.05). Moreover, high expression of EZH2 in ovarian carcinoma was determined to be a strong and an independent predictor of short overall survival (P < 0.05). In ovarian carcinoma HO-8910 and UACC-326 cell lines, EZH2 knockdown by RNA interference led to a G 1 phase cell cycle arrest, reduced cell growth/proliferation and inhibited cell migration and/or invasion in vitro. In addition, EZH2 knockdown was found to reduce transforming growth factor-β1 (TGF-β1) expression and increase E-cadherin expression either in the transcript or in the protein levels. Furthermore, a significant positive correlation between overexpression of EZH2 and TGF-β1 in ovarian carcinoma tissues was observed (P < 0.001). These findings suggest a potential important role of EZH2 in the control of cell migration and/or invasion via the regulation of TGF-β1 expression, and the high expression of EZH2, as detected by IHC, is an independent molecular marker for shortened survival time of patients with ovarian carcinoma. © The Author 2010. Published by Oxford University Press. All rights reserved. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Carcinogenesis, 2010, v. 31 n. 9, p. 1576-1583 [How to Cite?] DOI: http://dx.doi.org/10.1093/carcin/bgq150 | ||||||
| dc.identifier.citeulike | 7842375 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1093/carcin/bgq150 | ||||||
| dc.identifier.epage | 1583 | ||||||
| dc.identifier.isi | WOS:000281530400010
Funding Information: Major State Basic Research Program of China (2006CB910104 and 2010CB529401); Nature Science Foundation of China (30772334). | ||||||
| dc.identifier.issn | 0143-3334 2011 Impact Factor: 5.702 2011 SCImago Journal Rankings: 0.692 | ||||||
| dc.identifier.issue | 9 | ||||||
| dc.identifier.pmid | 20668008 | ||||||
| dc.identifier.scopus | eid_2-s2.0-77956277833 | ||||||
| dc.identifier.spage | 1576 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/150831 | ||||||
| dc.identifier.volume | 31 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Oxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/ | ||||||
| dc.publisher.place | United Kingdom | ||||||
| dc.relation.ispartof | Carcinogenesis | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Adenocarcinoma, Mucinous - Genetics - Metabolism - Pathology | ||||||
| dc.subject.mesh | Adolescent | ||||||
| dc.subject.mesh | Adult | ||||||
| dc.subject.mesh | Aged | ||||||
| dc.subject.mesh | Aged, 80 And Over | ||||||
| dc.subject.mesh | Blotting, Western | ||||||
| dc.subject.mesh | Cell Adhesion | ||||||
| dc.subject.mesh | Cell Cycle | ||||||
| dc.subject.mesh | Cell Movement | ||||||
| dc.subject.mesh | Cystadenocarcinoma, Serous - Genetics - Metabolism - Pathology | ||||||
| dc.subject.mesh | Dna-Binding Proteins - Physiology | ||||||
| dc.subject.mesh | Female | ||||||
| dc.subject.mesh | Gene Expression Regulation, Neoplastic | ||||||
| dc.subject.mesh | Humans | ||||||
| dc.subject.mesh | Immunoenzyme Techniques | ||||||
| dc.subject.mesh | In Situ Hybridization, Fluorescence | ||||||
| dc.subject.mesh | Middle Aged | ||||||
| dc.subject.mesh | Neoplasm Invasiveness | ||||||
| dc.subject.mesh | Neoplasm Metastasis | ||||||
| dc.subject.mesh | Neoplasm Staging | ||||||
| dc.subject.mesh | Ovarian Neoplasms - Genetics - Metabolism - Pathology | ||||||
| dc.subject.mesh | Rna, Messenger - Genetics | ||||||
| dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | ||||||
| dc.subject.mesh | Survival Rate | ||||||
| dc.subject.mesh | Tissue Array Analysis | ||||||
| dc.subject.mesh | Transcription Factors - Physiology | ||||||
| dc.subject.mesh | Transforming Growth Factor Beta1 - Genetics - Metabolism | ||||||
| dc.subject.mesh | Tumor Cells, Cultured | ||||||
| dc.subject.mesh | Young Adult | ||||||
| dc.title | EZH2 supports ovarian carcinoma cell invasion and/or metastasis via regulation of TGF-β 1 and is a predictor of outcome in ovarian carcinoma patients | ||||||
| dc.type | Article |
Author Affiliations
- Sun Yat-Sen University
- Chinese University of Hong Kong