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Article: Expression of EIF-5A2 gene in ovarian carcinoma and its clinical significance

TitleExpression of EIF-5A2 gene in ovarian carcinoma and its clinical significance
Authors
KeywordsEif-5A2 Gene
Fluorescence In Situ Hybridization
Immunohistochemistry
Tissue Microarray
Tumor Of The Ovary
Issue Date2010
PublisherTianjin Yike Daxue Fushu Zhongliu Yiyuan. The Journal's web site is located at http://zgzllc-e.periodicals.net.cn/
Citation
Chinese Journal Of Clinical Oncology, 2010, v. 37 n. 14, p. 796-799 How to Cite?
AbstractObjective: To investigate the expression and amplification of eukaryotic translation initiation factor 5A2 (EIF-5A2) gene in epithelial tumor of the ovary and its clinical significance. Methods: Immunohistochemistry and fluorescence in situ hybridization, in combination with tissue microarray, were used to examine the protein expression and amplification of EIF-5A2 in 50 ovarian adenomas, 50 borderline tumors of the ovary and 150 ovarian carcinomas to evaluate the potential associations between EIF-5A2 expression and patient's clinico-pathologic parameters in ovarian carcinoma cohorts. Results: In the immunohistochemical assay, over-expression of EIF-5A2 protein occurred in 6.4% of benign ovarian adenomas, 28.3% borderline tumors and 56.6% ovarian carcinomas. In ovarian carcinomas, there was a significant correlation in EIF-5A2 expression, Silverberg's grading and FIGO staging (P<0.05), in which the over-expression of EIF-5A2 was observed in 70.0% of high-grade (G3) carcinomas, with the positive rate significantly higher than that in the G1/G2 carcinomas (49.5%). This over-expression also occurred in 65.6% of the carcinomas with late clinical stages (FIGO stage III/IV), with a significantly higher positive rate compared to that in the tumors with FIGO stage I/II (38.3%). Furthermore, there was a significant positive correlation between EIF-5A2 over-expression and cell proliferation (the levels of Ki-67 expression) in our ovarian carcinomas (P<0.01), in which higher expression of Ki-67 was found in the majority of carcinomas with over-expression of EIF-5A2 (72.8%), while lower expression of Ki-67 was seen in the majority of carcinomas with normal expression of EIF-5A2 (66.1%). It was shown by fluorescence in situ hybridization assay that amplification of EIF-5A2 gene was present in only 13.8% of the ovarian carcinomas. EIF-5A2 amplification was not observed in the borderline or the benign ovarian tumors. Conclusion: Over-expression of EIF-5A2 protein may possibly, at least in part, play an important role in the tumorigenesis of epithelial tumors of the ovary through its effect of promoting cell proliferation. Moreover, it is in close correlation with the malignant histological phenotype and/or invasive process of ovarian carcinomas.
Persistent Identifierhttp://hdl.handle.net/10722/150830
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Len_US
dc.contributor.authorYang, Gen_US
dc.contributor.authorHe, Len_US
dc.contributor.authorLi, Len_US
dc.contributor.authorLiao, Yen_US
dc.contributor.authorGuan, Xen_US
dc.contributor.authorXie, Den_US
dc.date.accessioned2012-06-26T06:11:40Z-
dc.date.available2012-06-26T06:11:40Z-
dc.date.issued2010en_US
dc.identifier.citationChinese Journal Of Clinical Oncology, 2010, v. 37 n. 14, p. 796-799en_US
dc.identifier.issn1672-7118en_US
dc.identifier.urihttp://hdl.handle.net/10722/150830-
dc.description.abstractObjective: To investigate the expression and amplification of eukaryotic translation initiation factor 5A2 (EIF-5A2) gene in epithelial tumor of the ovary and its clinical significance. Methods: Immunohistochemistry and fluorescence in situ hybridization, in combination with tissue microarray, were used to examine the protein expression and amplification of EIF-5A2 in 50 ovarian adenomas, 50 borderline tumors of the ovary and 150 ovarian carcinomas to evaluate the potential associations between EIF-5A2 expression and patient's clinico-pathologic parameters in ovarian carcinoma cohorts. Results: In the immunohistochemical assay, over-expression of EIF-5A2 protein occurred in 6.4% of benign ovarian adenomas, 28.3% borderline tumors and 56.6% ovarian carcinomas. In ovarian carcinomas, there was a significant correlation in EIF-5A2 expression, Silverberg's grading and FIGO staging (P<0.05), in which the over-expression of EIF-5A2 was observed in 70.0% of high-grade (G3) carcinomas, with the positive rate significantly higher than that in the G1/G2 carcinomas (49.5%). This over-expression also occurred in 65.6% of the carcinomas with late clinical stages (FIGO stage III/IV), with a significantly higher positive rate compared to that in the tumors with FIGO stage I/II (38.3%). Furthermore, there was a significant positive correlation between EIF-5A2 over-expression and cell proliferation (the levels of Ki-67 expression) in our ovarian carcinomas (P<0.01), in which higher expression of Ki-67 was found in the majority of carcinomas with over-expression of EIF-5A2 (72.8%), while lower expression of Ki-67 was seen in the majority of carcinomas with normal expression of EIF-5A2 (66.1%). It was shown by fluorescence in situ hybridization assay that amplification of EIF-5A2 gene was present in only 13.8% of the ovarian carcinomas. EIF-5A2 amplification was not observed in the borderline or the benign ovarian tumors. Conclusion: Over-expression of EIF-5A2 protein may possibly, at least in part, play an important role in the tumorigenesis of epithelial tumors of the ovary through its effect of promoting cell proliferation. Moreover, it is in close correlation with the malignant histological phenotype and/or invasive process of ovarian carcinomas.en_US
dc.languageengen_US
dc.publisherTianjin Yike Daxue Fushu Zhongliu Yiyuan. The Journal's web site is located at http://zgzllc-e.periodicals.net.cn/en_US
dc.relation.ispartofChinese Journal of Clinical Oncologyen_US
dc.subjectEif-5A2 Geneen_US
dc.subjectFluorescence In Situ Hybridizationen_US
dc.subjectImmunohistochemistryen_US
dc.subjectTissue Microarrayen_US
dc.subjectTumor Of The Ovaryen_US
dc.titleExpression of EIF-5A2 gene in ovarian carcinoma and its clinical significanceen_US
dc.typeArticleen_US
dc.identifier.emailGuan, X:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, X=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3969/j.issn.1000-8179.2010.14.005en_US
dc.identifier.scopuseid_2-s2.0-77955861156en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955861156&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume37en_US
dc.identifier.issue14en_US
dc.identifier.spage796en_US
dc.identifier.epage799en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridYang, L=9843545700en_US
dc.identifier.scopusauthoridYang, G=16064823400en_US
dc.identifier.scopusauthoridHe, L=35069492500en_US
dc.identifier.scopusauthoridLi, L=36065125900en_US
dc.identifier.scopusauthoridLiao, Y=36114448500en_US
dc.identifier.scopusauthoridGuan, X=7201463221en_US
dc.identifier.scopusauthoridXie, D=35070710200en_US

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