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Article: Expression of EIF-5A2 gene in ovarian carcinoma and its clinical significance
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TitleExpression of EIF-5A2 gene in ovarian carcinoma and its clinical significance
 
AuthorsYang, L1
Yang, G2
He, L2
Li, L2
Liao, Y2
Guan, X2
Xie, D2
 
KeywordsEif-5A2 Gene
Fluorescence In Situ Hybridization
Immunohistochemistry
Tissue Microarray
Tumor Of The Ovary
 
Issue Date2010
 
PublisherTianjin Yike Daxue Fushu Zhongliu Yiyuan. The Journal's web site is located at http://zgzllc-e.periodicals.net.cn/
 
CitationChinese Journal Of Clinical Oncology, 2010, v. 37 n. 14, p. 796-799 [How to Cite?]
DOI: http://dx.doi.org/10.3969/j.issn.1000-8179.2010.14.005
 
AbstractObjective: To investigate the expression and amplification of eukaryotic translation initiation factor 5A2 (EIF-5A2) gene in epithelial tumor of the ovary and its clinical significance. Methods: Immunohistochemistry and fluorescence in situ hybridization, in combination with tissue microarray, were used to examine the protein expression and amplification of EIF-5A2 in 50 ovarian adenomas, 50 borderline tumors of the ovary and 150 ovarian carcinomas to evaluate the potential associations between EIF-5A2 expression and patient's clinico-pathologic parameters in ovarian carcinoma cohorts. Results: In the immunohistochemical assay, over-expression of EIF-5A2 protein occurred in 6.4% of benign ovarian adenomas, 28.3% borderline tumors and 56.6% ovarian carcinomas. In ovarian carcinomas, there was a significant correlation in EIF-5A2 expression, Silverberg's grading and FIGO staging (P<0.05), in which the over-expression of EIF-5A2 was observed in 70.0% of high-grade (G3) carcinomas, with the positive rate significantly higher than that in the G1/G2 carcinomas (49.5%). This over-expression also occurred in 65.6% of the carcinomas with late clinical stages (FIGO stage III/IV), with a significantly higher positive rate compared to that in the tumors with FIGO stage I/II (38.3%). Furthermore, there was a significant positive correlation between EIF-5A2 over-expression and cell proliferation (the levels of Ki-67 expression) in our ovarian carcinomas (P<0.01), in which higher expression of Ki-67 was found in the majority of carcinomas with over-expression of EIF-5A2 (72.8%), while lower expression of Ki-67 was seen in the majority of carcinomas with normal expression of EIF-5A2 (66.1%). It was shown by fluorescence in situ hybridization assay that amplification of EIF-5A2 gene was present in only 13.8% of the ovarian carcinomas. EIF-5A2 amplification was not observed in the borderline or the benign ovarian tumors. Conclusion: Over-expression of EIF-5A2 protein may possibly, at least in part, play an important role in the tumorigenesis of epithelial tumors of the ovary through its effect of promoting cell proliferation. Moreover, it is in close correlation with the malignant histological phenotype and/or invasive process of ovarian carcinomas.
 
ISSN1672-7118
 
DOIhttp://dx.doi.org/10.3969/j.issn.1000-8179.2010.14.005
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYang, L
 
dc.contributor.authorYang, G
 
dc.contributor.authorHe, L
 
dc.contributor.authorLi, L
 
dc.contributor.authorLiao, Y
 
dc.contributor.authorGuan, X
 
dc.contributor.authorXie, D
 
dc.date.accessioned2012-06-26T06:11:40Z
 
dc.date.available2012-06-26T06:11:40Z
 
dc.date.issued2010
 
dc.description.abstractObjective: To investigate the expression and amplification of eukaryotic translation initiation factor 5A2 (EIF-5A2) gene in epithelial tumor of the ovary and its clinical significance. Methods: Immunohistochemistry and fluorescence in situ hybridization, in combination with tissue microarray, were used to examine the protein expression and amplification of EIF-5A2 in 50 ovarian adenomas, 50 borderline tumors of the ovary and 150 ovarian carcinomas to evaluate the potential associations between EIF-5A2 expression and patient's clinico-pathologic parameters in ovarian carcinoma cohorts. Results: In the immunohistochemical assay, over-expression of EIF-5A2 protein occurred in 6.4% of benign ovarian adenomas, 28.3% borderline tumors and 56.6% ovarian carcinomas. In ovarian carcinomas, there was a significant correlation in EIF-5A2 expression, Silverberg's grading and FIGO staging (P<0.05), in which the over-expression of EIF-5A2 was observed in 70.0% of high-grade (G3) carcinomas, with the positive rate significantly higher than that in the G1/G2 carcinomas (49.5%). This over-expression also occurred in 65.6% of the carcinomas with late clinical stages (FIGO stage III/IV), with a significantly higher positive rate compared to that in the tumors with FIGO stage I/II (38.3%). Furthermore, there was a significant positive correlation between EIF-5A2 over-expression and cell proliferation (the levels of Ki-67 expression) in our ovarian carcinomas (P<0.01), in which higher expression of Ki-67 was found in the majority of carcinomas with over-expression of EIF-5A2 (72.8%), while lower expression of Ki-67 was seen in the majority of carcinomas with normal expression of EIF-5A2 (66.1%). It was shown by fluorescence in situ hybridization assay that amplification of EIF-5A2 gene was present in only 13.8% of the ovarian carcinomas. EIF-5A2 amplification was not observed in the borderline or the benign ovarian tumors. Conclusion: Over-expression of EIF-5A2 protein may possibly, at least in part, play an important role in the tumorigenesis of epithelial tumors of the ovary through its effect of promoting cell proliferation. Moreover, it is in close correlation with the malignant histological phenotype and/or invasive process of ovarian carcinomas.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationChinese Journal Of Clinical Oncology, 2010, v. 37 n. 14, p. 796-799 [How to Cite?]
DOI: http://dx.doi.org/10.3969/j.issn.1000-8179.2010.14.005
 
dc.identifier.doihttp://dx.doi.org/10.3969/j.issn.1000-8179.2010.14.005
 
dc.identifier.epage799
 
dc.identifier.issn1672-7118
 
dc.identifier.issue14
 
dc.identifier.scopuseid_2-s2.0-77955861156
 
dc.identifier.spage796
 
dc.identifier.urihttp://hdl.handle.net/10722/150830
 
dc.identifier.volume37
 
dc.languageeng
 
dc.publisherTianjin Yike Daxue Fushu Zhongliu Yiyuan. The Journal's web site is located at http://zgzllc-e.periodicals.net.cn/
 
dc.publisher.placeChina
 
dc.relation.ispartofChinese Journal of Clinical Oncology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectEif-5A2 Gene
 
dc.subjectFluorescence In Situ Hybridization
 
dc.subjectImmunohistochemistry
 
dc.subjectTissue Microarray
 
dc.subjectTumor Of The Ovary
 
dc.titleExpression of EIF-5A2 gene in ovarian carcinoma and its clinical significance
 
dc.typeArticle
 
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<contributor.author>Yang, G</contributor.author>
<contributor.author>He, L</contributor.author>
<contributor.author>Li, L</contributor.author>
<contributor.author>Liao, Y</contributor.author>
<contributor.author>Guan, X</contributor.author>
<contributor.author>Xie, D</contributor.author>
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<description.abstract>Objective: To investigate the expression and amplification of eukaryotic translation initiation factor 5A2 (EIF-5A2) gene in epithelial tumor of the ovary and its clinical significance. Methods: Immunohistochemistry and fluorescence in situ hybridization, in combination with tissue microarray, were used to examine the protein expression and amplification of EIF-5A2 in 50 ovarian adenomas, 50 borderline tumors of the ovary and 150 ovarian carcinomas to evaluate the potential associations between EIF-5A2 expression and patient&apos;s clinico-pathologic parameters in ovarian carcinoma cohorts. Results: In the immunohistochemical assay, over-expression of EIF-5A2 protein occurred in 6.4% of benign ovarian adenomas, 28.3% borderline tumors and 56.6% ovarian carcinomas. In ovarian carcinomas, there was a significant correlation in EIF-5A2 expression, Silverberg&apos;s grading and FIGO staging (P&lt;0.05), in which the over-expression of EIF-5A2 was observed in 70.0% of high-grade (G3) carcinomas, with the positive rate significantly higher than that in the G1/G2 carcinomas (49.5%). This over-expression also occurred in 65.6% of the carcinomas with late clinical stages (FIGO stage III/IV), with a significantly higher positive rate compared to that in the tumors with FIGO stage I/II (38.3%). Furthermore, there was a significant positive correlation between EIF-5A2 over-expression and cell proliferation (the levels of Ki-67 expression) in our ovarian carcinomas (P&lt;0.01), in which higher expression of Ki-67 was found in the majority of carcinomas with over-expression of EIF-5A2 (72.8%), while lower expression of Ki-67 was seen in the majority of carcinomas with normal expression of EIF-5A2 (66.1%). It was shown by fluorescence in situ hybridization assay that amplification of EIF-5A2 gene was present in only 13.8% of the ovarian carcinomas. EIF-5A2 amplification was not observed in the borderline or the benign ovarian tumors. Conclusion: Over-expression of EIF-5A2 protein may possibly, at least in part, play an important role in the tumorigenesis of epithelial tumors of the ovary through its effect of promoting cell proliferation. Moreover, it is in close correlation with the malignant histological phenotype and/or invasive process of ovarian carcinomas.</description.abstract>
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Author Affiliations
  1. Guangzhou Women and Children's Hospital
  2. Sun Yat-Sen University