File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Overexpression of EIF-5A2 is an independent predictor of outcome in patients of urothelial carcinoma of the bladder treated with radical cystectomy

TitleOverexpression of EIF-5A2 is an independent predictor of outcome in patients of urothelial carcinoma of the bladder treated with radical cystectomy
Authors
Issue Date2009
Citation
Cancer Epidemiology Biomarkers And Prevention, 2009, v. 18 n. 2, p. 400-408 How to Cite?
AbstractBackground: Our previous study has suggested an oncogenic role of eIF-5A2 in ovarian tumorigenesis. Abnormalities of eIF-5A2 and its clinical/prognostic significance, however, in urothelial carcinoma of the bladder (UC) are unclear. Methods: In this study, the methods of reverse transcription-PCR, immunohistochemistry, and fluorescence in situ hybridization were used to examine mRNA/protein expression and amplification of eIF-5A2 in a large cohort of UCs treated with radical cystectomy. Results: Up-regulated expression of eIF-5A2 mRNA was observed in 50% (8 of 16) of UCs, when compared with adjacent normal bladder tissues. Overexpression of EIF-5A2 protein and amplification of eIF-5A2 was examined informatively in 45.3% (39 of 86) and 10.6% (5 of 47) of UCs, respectively. In univariate survival analysis of the UC cohorts, a significant association of overexpression of EIF-5A2 with shortened patient survival (mean, 38.2 months versus 52.9 months, P = 0.001, log-rank test) was shown. In different subsets of UC patients, overexpression of EIF-5A2 was also a prognostic indicator in grade 1/2 (P = 0.0009) and grade 3 (P = 0.016) tumor patients, and in pT1 (P = 0.0089), pT2 (P = 0.0354), pT3/4 (P = 0.0058), pN0 (P = 0.0039), and pN1-2 (P = 0.0093) tumor patients. Importantly, EIF-5A2 expression (P = 0.0007) together with pT stage (P = 0.0001) provided significant independent prognostic variables in multivariate analysis. Conclusions: These findings indicate that overexpression of EIF-5A2 in UCs is coincident with acquisition of a poor prognostic phenotype, suggesting that the expression of EIF-5A2, as detected by immunohisto-chemistry, is an independent molecular marker for shortened survival time of UC patients treated with radical cystectomy. Copyright © 2009 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/150826
ISSN
2015 Impact Factor: 3.622
2015 SCImago Journal Rankings: 2.579
ISI Accession Number ID
Funding AgencyGrant Number
Major State Basic Research program of China2006CB910104
National Nature Science Foundation of China30772475
Guangdong Provincial Science and Technology Foundation2008B030301112
Hong Kong Research Grant Council GrantHKU7656/07M
Funding Information:

Grant support: Major State Basic Research program of China (2006CB910104); the National Nature Science Foundation of China (No. 30772475); Guangdong Provincial Science and Technology Foundation (2008B030301112) and Hong Kong Research Grant Council Grant (HKU7656/07M).

References

 

DC FieldValueLanguage
dc.contributor.authorChen, Wen_US
dc.contributor.authorLuo, JHen_US
dc.contributor.authorHua, WFen_US
dc.contributor.authorZhou, FJen_US
dc.contributor.authorLin, MCen_US
dc.contributor.authorKung, HFen_US
dc.contributor.authorZeng, YXen_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorXie, Den_US
dc.date.accessioned2012-06-26T06:11:34Z-
dc.date.available2012-06-26T06:11:34Z-
dc.date.issued2009en_US
dc.identifier.citationCancer Epidemiology Biomarkers And Prevention, 2009, v. 18 n. 2, p. 400-408en_US
dc.identifier.issn1055-9965en_US
dc.identifier.urihttp://hdl.handle.net/10722/150826-
dc.description.abstractBackground: Our previous study has suggested an oncogenic role of eIF-5A2 in ovarian tumorigenesis. Abnormalities of eIF-5A2 and its clinical/prognostic significance, however, in urothelial carcinoma of the bladder (UC) are unclear. Methods: In this study, the methods of reverse transcription-PCR, immunohistochemistry, and fluorescence in situ hybridization were used to examine mRNA/protein expression and amplification of eIF-5A2 in a large cohort of UCs treated with radical cystectomy. Results: Up-regulated expression of eIF-5A2 mRNA was observed in 50% (8 of 16) of UCs, when compared with adjacent normal bladder tissues. Overexpression of EIF-5A2 protein and amplification of eIF-5A2 was examined informatively in 45.3% (39 of 86) and 10.6% (5 of 47) of UCs, respectively. In univariate survival analysis of the UC cohorts, a significant association of overexpression of EIF-5A2 with shortened patient survival (mean, 38.2 months versus 52.9 months, P = 0.001, log-rank test) was shown. In different subsets of UC patients, overexpression of EIF-5A2 was also a prognostic indicator in grade 1/2 (P = 0.0009) and grade 3 (P = 0.016) tumor patients, and in pT1 (P = 0.0089), pT2 (P = 0.0354), pT3/4 (P = 0.0058), pN0 (P = 0.0039), and pN1-2 (P = 0.0093) tumor patients. Importantly, EIF-5A2 expression (P = 0.0007) together with pT stage (P = 0.0001) provided significant independent prognostic variables in multivariate analysis. Conclusions: These findings indicate that overexpression of EIF-5A2 in UCs is coincident with acquisition of a poor prognostic phenotype, suggesting that the expression of EIF-5A2, as detected by immunohisto-chemistry, is an independent molecular marker for shortened survival time of UC patients treated with radical cystectomy. Copyright © 2009 American Association for Cancer Research.en_US
dc.languageengen_US
dc.relation.ispartofCancer Epidemiology Biomarkers and Preventionen_US
dc.subject.meshAgeden_US
dc.subject.meshChi-Square Distributionen_US
dc.subject.meshCystectomy - Methodsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshKi-67 Antigen - Biosynthesisen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroarray Analysisen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeptide Initiation Factors - Metabolismen_US
dc.subject.meshPredictive Value Of Testsen_US
dc.subject.meshPrognosisen_US
dc.subject.meshProportional Hazards Modelsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSurvival Analysisen_US
dc.subject.meshTumor Markers, Biological - Genetics - Metabolismen_US
dc.subject.meshUp-Regulationen_US
dc.subject.meshUrinary Bladder Neoplasms - Genetics - Metabolism - Pathology - Surgeryen_US
dc.titleOverexpression of EIF-5A2 is an independent predictor of outcome in patients of urothelial carcinoma of the bladder treated with radical cystectomyen_US
dc.typeArticleen_US
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityLin, MC=rp00746en_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1158/1055-9965.EPI-08-0754en_US
dc.identifier.pmid19155439-
dc.identifier.scopuseid_2-s2.0-60549089824en_US
dc.identifier.hkuros154324-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60549089824&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume18en_US
dc.identifier.issue2en_US
dc.identifier.spage400en_US
dc.identifier.epage408en_US
dc.identifier.isiWOS:000263547800006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChen, W=16033282000en_US
dc.identifier.scopusauthoridLuo, JH=7404183419en_US
dc.identifier.scopusauthoridHua, WF=24401204900en_US
dc.identifier.scopusauthoridZhou, FJ=34978063300en_US
dc.identifier.scopusauthoridLin, MC=7404816359en_US
dc.identifier.scopusauthoridKung, HF=7402514190en_US
dc.identifier.scopusauthoridZeng, YX=7402981579en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridXie, D=35070710200en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats