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Article: Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2

TitleComparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2
人鼻咽癌順鉑耐藥細胞系(CNE2/DDP)及其親代細胞(CNE2)的比較基因組雜交研究
Authors
Issue Date2004
PublisherSun Yat-sen University Cancer Center (中山醫科大學腫瘤防治中心). The Journal's web site is located at http://www.cjcsysu.cn/
Citation
Chinese Journal Of Cancer, 2004, v. 23 n. 4, p. 386-390 How to Cite?
癌症, 2004, v. 23 n. 4, p. 386-390 How to Cite?
AbstractBACKGROUND & OBJECTIVE: Developed from fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH) is a new molecular and cellular technique,used to examine the genomic imbalances,especially the loss and amplification of chromosomes,and to locate these alterations in certain chromosome regions. For a comprehensive understanding of the possible differences in genomic DNA between nasopharyngeal carcinoma drug-resistant and drug-sensitive cells, we analyzed the genomic DNA of a nasopharyngeal carcinoma drug-resistant cell line CNE2/DDP and its parent cell line CNE2 with CGH. METHODS: Genomic DNA was extracted from CNE2/DDP and CNE2 cells, as well as from normal placenta tissue. Fluorescent random primed labeling method was used to label the DNA probes (CNE2 and CNE2/DDP cells with green fluorescein-12-dUTP and normal placenta tissue with red tetramethylrhodamine-5-dUTP).The labeled DNA probes were then co-hybridized with normal lymphocyte metaphase chromosomes. Signals were taken by charge coupled device (CCD) and processed with Quips CGH Program after fluorescent hybridization. The green-to-red fluorescent ratio was calculated automatically and showed with graphs. RESULTS: There were extensive chromosome changes in CNE2, mainly the gain of 1q, 3q, 5p, 6p, 7p, 8q, 9q, 11p, 12q and 19q, and the loss of 4q, 12p, 13p, 14p, 15p, 18, 20q, 21p and 22. However, the CNE2/DDP cells, which come from the CNE2, showed a relatively normal karyotype except loss of 8p and gain of 8q and 19q. Consistent result was achieved after the CNE2/DDP cells were cultured in the medium free of any drugs for over one month. It appears that the CNE2/DDP cell has relative normal and much more stable chromosome constitution than its parent CNE2 cell. CONCLUSION: The CNE2/DDP is a single drug-resistant clone selected from CNE2,which is a blend of sub-clones that have different sorts of chromosome number and structure. It strongly suggests that the appearance of tumor drug-resistance is mainly a select process in which the would-be drug-resistant cell clone be selected from unfavorable survival condition.
本文報告四會縣醃菜二氯甲烷提取液的誘變性試驗。醃菜樣本提取液Ⅰ號引起姐妹染色單體交換(SCE)率及微核(MN)率顯著升高,醃菜樣本提取液Ⅱ號亦引起SCE率明顯升高,但MN率僅略有升高。化學分析表明,該兩份醃菜的揮發性亞硝胺含量分別為37.83、33.38 ppb。醃菜提取液的誘變性似不能單用亞硝胺來解釋。實驗結果提示,鼻咽癌高發區四會縣醃菜中可能含有除揮發性亞硝胺以外的其他誘變性物質。
Persistent Identifierhttp://hdl.handle.net/10722/150816
ISSN
2015 Impact Factor: 2.814
2015 SCImago Journal Rankings: 1.081

 

DC FieldValueLanguage
dc.contributor.authorJiang, RDen_US
dc.contributor.authorHu, Len_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorZhang, LXen_US
dc.contributor.authorYue, Wen_US
dc.contributor.authorCen, XTen_US
dc.contributor.authorLi, CHen_US
dc.date.accessioned2012-06-26T06:11:09Z-
dc.date.available2012-06-26T06:11:09Z-
dc.date.issued2004en_US
dc.identifier.citationChinese Journal Of Cancer, 2004, v. 23 n. 4, p. 386-390en_US
dc.identifier.citation癌症, 2004, v. 23 n. 4, p. 386-390-
dc.identifier.issn1000-467Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/150816-
dc.description.abstractBACKGROUND & OBJECTIVE: Developed from fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH) is a new molecular and cellular technique,used to examine the genomic imbalances,especially the loss and amplification of chromosomes,and to locate these alterations in certain chromosome regions. For a comprehensive understanding of the possible differences in genomic DNA between nasopharyngeal carcinoma drug-resistant and drug-sensitive cells, we analyzed the genomic DNA of a nasopharyngeal carcinoma drug-resistant cell line CNE2/DDP and its parent cell line CNE2 with CGH. METHODS: Genomic DNA was extracted from CNE2/DDP and CNE2 cells, as well as from normal placenta tissue. Fluorescent random primed labeling method was used to label the DNA probes (CNE2 and CNE2/DDP cells with green fluorescein-12-dUTP and normal placenta tissue with red tetramethylrhodamine-5-dUTP).The labeled DNA probes were then co-hybridized with normal lymphocyte metaphase chromosomes. Signals were taken by charge coupled device (CCD) and processed with Quips CGH Program after fluorescent hybridization. The green-to-red fluorescent ratio was calculated automatically and showed with graphs. RESULTS: There were extensive chromosome changes in CNE2, mainly the gain of 1q, 3q, 5p, 6p, 7p, 8q, 9q, 11p, 12q and 19q, and the loss of 4q, 12p, 13p, 14p, 15p, 18, 20q, 21p and 22. However, the CNE2/DDP cells, which come from the CNE2, showed a relatively normal karyotype except loss of 8p and gain of 8q and 19q. Consistent result was achieved after the CNE2/DDP cells were cultured in the medium free of any drugs for over one month. It appears that the CNE2/DDP cell has relative normal and much more stable chromosome constitution than its parent CNE2 cell. CONCLUSION: The CNE2/DDP is a single drug-resistant clone selected from CNE2,which is a blend of sub-clones that have different sorts of chromosome number and structure. It strongly suggests that the appearance of tumor drug-resistance is mainly a select process in which the would-be drug-resistant cell clone be selected from unfavorable survival condition.en_US
dc.description.abstract本文報告四會縣醃菜二氯甲烷提取液的誘變性試驗。醃菜樣本提取液Ⅰ號引起姐妹染色單體交換(SCE)率及微核(MN)率顯著升高,醃菜樣本提取液Ⅱ號亦引起SCE率明顯升高,但MN率僅略有升高。化學分析表明,該兩份醃菜的揮發性亞硝胺含量分別為37.83、33.38 ppb。醃菜提取液的誘變性似不能單用亞硝胺來解釋。實驗結果提示,鼻咽癌高發區四會縣醃菜中可能含有除揮發性亞硝胺以外的其他誘變性物質。-
dc.languagechien_US
dc.publisherSun Yat-sen University Cancer Center (中山醫科大學腫瘤防治中心). The Journal's web site is located at http://www.cjcsysu.cn/-
dc.relation.ispartofChinese Journal of Canceren_US
dc.relation.ispartof癌症-
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshChromosome Aberrationsen_US
dc.subject.meshDrug Resistance, Neoplasmen_US
dc.subject.meshHumansen_US
dc.subject.meshNasopharyngeal Neoplasms - Drug Therapy - Genetics - Pathologyen_US
dc.subject.meshNucleic Acid Hybridization - Methodsen_US
dc.titleComparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2en_US
dc.title人鼻咽癌順鉑耐藥細胞系(CNE2/DDP)及其親代細胞(CNE2)的比較基因組雜交研究-
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid15087024-
dc.identifier.scopuseid_2-s2.0-4043148783en_US
dc.identifier.volume23en_US
dc.identifier.issue4en_US
dc.identifier.spage386en_US
dc.identifier.epage390en_US
dc.publisher.placeChina (中國)-
dc.identifier.scopusauthoridJiang, RD=7202306980en_US
dc.identifier.scopusauthoridHu, L=34770075600en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridZhang, LX=36062518100en_US
dc.identifier.scopusauthoridYue, W=8712410700en_US
dc.identifier.scopusauthoridCen, XT=36927223600en_US
dc.identifier.scopusauthoridLi, CH=7501676450en_US
dc.customcontrol.immutablecsl 150108-

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