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Article: Apoptosis and expression of cytokines triggered by pyropheophorbide-a methyl ester-mediated photodynamic therapy in nasopharyngeal carcinoma cells

TitleApoptosis and expression of cytokines triggered by pyropheophorbide-a methyl ester-mediated photodynamic therapy in nasopharyngeal carcinoma cells
Authors
KeywordsApoptosis
Mitochondrial Membrane Potential
Nasopharyngeal Carcinoma
Photodynamic Therapy
Pyropheophorbide-A Methyl Ester
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/pdpdt
Citation
Photodiagnosis And Photodynamic Therapy, 2006, v. 3 n. 4, p. 247-258 How to Cite?
AbstractThe photodynamic properties of pyropheophorbide-a methyl ester (MPPa), a semi-synthetic photosensitizer derived from chlorophyll a, were evaluated in a human nasopharyngeal carcinoma HONE-1 cell line. MPPa was non-toxic to the HONE-1. At the concentrations of 0.5-2 μM, MPPa-mediated a drug dose-dependent photocytotoxicity in the HONE-1 cells. Confocal microscopy revealed a subcellular localization of MPPa in mitochondria and the Golgi apparatus. MPPa PDT-induced apoptosis was associated with the collapse of mitochondrial membrane potential, release of cytochrome c, the up-regulation of endoplasmic reticulum (ER) stress proteins (calnexin, Grp 94 and Grp78), and the activation of caspases-3 and -9. The photocytotoxicity was reduced by the corresponding specific caspase inhibitors. MPPa PDT-treated HONE-1 cells also up-regulated the gene expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and beta-chemokines (MIP-1β, MPIF-1, and MPIF-2). These results suggest that the MPPa may be developed as a chlorophyll-based photosensitizer for the treatment of nasopharyngeal carcinoma. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/150806
ISSN
2015 Impact Factor: 2.412
2015 SCImago Journal Rankings: 0.678
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, KMen_US
dc.contributor.authorSun, Xen_US
dc.contributor.authorKoon, HKen_US
dc.contributor.authorLeung, WNen_US
dc.contributor.authorFung, MCen_US
dc.contributor.authorWong, RNSen_US
dc.contributor.authorLung, MLen_US
dc.contributor.authorChang, CKen_US
dc.contributor.authorMak, NKen_US
dc.date.accessioned2012-06-26T06:10:49Z-
dc.date.available2012-06-26T06:10:49Z-
dc.date.issued2006en_US
dc.identifier.citationPhotodiagnosis And Photodynamic Therapy, 2006, v. 3 n. 4, p. 247-258en_US
dc.identifier.issn1572-1000en_US
dc.identifier.urihttp://hdl.handle.net/10722/150806-
dc.description.abstractThe photodynamic properties of pyropheophorbide-a methyl ester (MPPa), a semi-synthetic photosensitizer derived from chlorophyll a, were evaluated in a human nasopharyngeal carcinoma HONE-1 cell line. MPPa was non-toxic to the HONE-1. At the concentrations of 0.5-2 μM, MPPa-mediated a drug dose-dependent photocytotoxicity in the HONE-1 cells. Confocal microscopy revealed a subcellular localization of MPPa in mitochondria and the Golgi apparatus. MPPa PDT-induced apoptosis was associated with the collapse of mitochondrial membrane potential, release of cytochrome c, the up-regulation of endoplasmic reticulum (ER) stress proteins (calnexin, Grp 94 and Grp78), and the activation of caspases-3 and -9. The photocytotoxicity was reduced by the corresponding specific caspase inhibitors. MPPa PDT-treated HONE-1 cells also up-regulated the gene expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and beta-chemokines (MIP-1β, MPIF-1, and MPIF-2). These results suggest that the MPPa may be developed as a chlorophyll-based photosensitizer for the treatment of nasopharyngeal carcinoma. © 2006 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/pdpdten_US
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapyen_US
dc.subjectApoptosisen_US
dc.subjectMitochondrial Membrane Potentialen_US
dc.subjectNasopharyngeal Carcinomaen_US
dc.subjectPhotodynamic Therapyen_US
dc.subjectPyropheophorbide-A Methyl Esteren_US
dc.titleApoptosis and expression of cytokines triggered by pyropheophorbide-a methyl ester-mediated photodynamic therapy in nasopharyngeal carcinoma cellsen_US
dc.typeArticleen_US
dc.identifier.emailLung, ML:mlilung@hku.hken_US
dc.identifier.authorityLung, ML=rp00300en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.pdpdt.2006.09.001en_US
dc.identifier.pmid25046989-
dc.identifier.scopuseid_2-s2.0-33845981082en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845981082&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume3en_US
dc.identifier.issue4en_US
dc.identifier.spage247en_US
dc.identifier.epage258en_US
dc.identifier.isiWOS:000208591500005-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLi, KM=11439529600en_US
dc.identifier.scopusauthoridSun, X=15758540300en_US
dc.identifier.scopusauthoridKoon, HK=12766487800en_US
dc.identifier.scopusauthoridLeung, WN=7201504470en_US
dc.identifier.scopusauthoridFung, MC=13310394400en_US
dc.identifier.scopusauthoridWong, RNS=7402126957en_US
dc.identifier.scopusauthoridLung, ML=7006411788en_US
dc.identifier.scopusauthoridChang, CK=7407039448en_US
dc.identifier.scopusauthoridMak, NK=35582657000en_US

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