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Article: The increase in mitochondrial association with actin precedes Bax translocation in apoptosis

TitleThe increase in mitochondrial association with actin precedes Bax translocation in apoptosis
Authors
Issue Date2006
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 2006, v. 396 n. 1, p. 1-5 How to Cite?
AbstractAccumulating evidence indicates the potential role of actin cytoskeleton in facilitating the mitochondrial recruitment of various pro-apoptotic proteins from the cytosol to initiate apoptosis. In the present paper, we report the observation of the increase in mitochondrial association of actin in early apoptosis. Using cell fractionation and Western blot analysis, we found that mitochondrial accumulation of β-actin occurred before the mitochondrial insertion of Bax and release of cytochrome c in apoptosis. The mitochondrial accumulation of β-actin was observed with various apoptotic stimuli in various cell lines, suggesting that this is a general apoptotic phenomenon in mammalian systems. Using fluorescence microscopy, we have shown that an apoptotic induction triggered the reorganization of the F-actin (filamentous actin) network with an increase in the association with mitochondria, which was observed before mitochondrial fission and nuclear condensation. Perhaps actin could contribute to the initiation of apoptosis by enabling cytosolic pro-apoptotic proteins to be carried to mitochondria by the cytoskeleton-driven trafficking system. © 2006 Biochemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/150802
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, HLen_US
dc.contributor.authorLe, AHPen_US
dc.contributor.authorLung, HLen_US
dc.date.accessioned2012-06-26T06:10:42Z-
dc.date.available2012-06-26T06:10:42Z-
dc.date.issued2006en_US
dc.identifier.citationBiochemical Journal, 2006, v. 396 n. 1, p. 1-5en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/150802-
dc.description.abstractAccumulating evidence indicates the potential role of actin cytoskeleton in facilitating the mitochondrial recruitment of various pro-apoptotic proteins from the cytosol to initiate apoptosis. In the present paper, we report the observation of the increase in mitochondrial association of actin in early apoptosis. Using cell fractionation and Western blot analysis, we found that mitochondrial accumulation of β-actin occurred before the mitochondrial insertion of Bax and release of cytochrome c in apoptosis. The mitochondrial accumulation of β-actin was observed with various apoptotic stimuli in various cell lines, suggesting that this is a general apoptotic phenomenon in mammalian systems. Using fluorescence microscopy, we have shown that an apoptotic induction triggered the reorganization of the F-actin (filamentous actin) network with an increase in the association with mitochondria, which was observed before mitochondrial fission and nuclear condensation. Perhaps actin could contribute to the initiation of apoptosis by enabling cytosolic pro-apoptotic proteins to be carried to mitochondria by the cytoskeleton-driven trafficking system. © 2006 Biochemical Society.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.meshActins - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Drug Effects - Physiology - Radiation Effectsen_US
dc.subject.meshCos Cells - Drug Effectsen_US
dc.subject.meshCell Line - Drug Effectsen_US
dc.subject.meshCercopithecus Aethiopsen_US
dc.subject.meshCulture Media, Serum-Free - Pharmacologyen_US
dc.subject.meshCytoskeleton - Physiology - Ultrastructureen_US
dc.subject.meshHela Cells - Drug Effects - Radiation Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen Peroxide - Pharmacologyen_US
dc.subject.meshKidney - Cytologyen_US
dc.subject.meshMitochondria - Physiologyen_US
dc.subject.meshProtein Transport - Drug Effects - Radiation Effectsen_US
dc.subject.meshStaurosporine - Pharmacologyen_US
dc.subject.meshUltraviolet Raysen_US
dc.subject.meshBcl-2-Associated X Protein - Metabolismen_US
dc.titleThe increase in mitochondrial association with actin precedes Bax translocation in apoptosisen_US
dc.typeArticleen_US
dc.identifier.emailLung, HL:hllung2@hku.hken_US
dc.identifier.authorityLung, HL=rp00299en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1042/BJ20060241en_US
dc.identifier.pmid16536728-
dc.identifier.scopuseid_2-s2.0-33646761645en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646761645&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume396en_US
dc.identifier.issue1en_US
dc.identifier.spage1en_US
dc.identifier.epage5en_US
dc.identifier.isiWOS:000237877900001-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTang, HL=13608168100en_US
dc.identifier.scopusauthoridLe, AHP=36725359300en_US
dc.identifier.scopusauthoridLung, HL=6603819904en_US

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