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Article: Establishment and cytogenetic characterization of a human hepatocellular carcinoma cell strain from the embolus in portal vein

TitleEstablishment and cytogenetic characterization of a human hepatocellular carcinoma cell strain from the embolus in portal vein
Authors
Issue Date2002
PublisherSun Yat-sen University Cancer Center (中山醫科大學腫瘤防治中心). The Journal's web site is located at http://www.cjcsysu.cn/
Citation
Chinese Journal of Cancer, 2002, v. 21 n. 11, p. 1203-1207 How to Cite?
癌症, 2002, v. 21 n. 11, p. 1203-1207 How to Cite?
AbstractBACKGROUND & OBJECTIVE: Poor prognosis of hepatocellular carcinoma (HCC) is due to its high recurrent rate after operation and early metastasis through portal vein. This study was designed to establish a metastatic HCC cell line and to investigate its molecular and cytogenetic characterization. METHODS: Cell culture was performed using the tissue obtained from a metastatic lesion in portal vein of a patient with HCC. After G-banding staining, karyotype, and comparative genomic hybridization (CGH) of the cultured H4M cells were characterized and cyclin D1 gene CCND1 was detected by differential PCR. RESULTS: The karyotype of metastatic HCC cell strain is a hypertriploid (71-78 chromosomes) with a huge marker chromosome containing a long homogeneously staining region (hsr). The main genetic alterations in H4M cells were a high copy number amplification of 11q13 and loss of 8p. Cyclin D1 gene CCND1 was also amplified significantly in the cells. CONCLUSIONS: The loss of chromosome 8p and high copy number amplification of 11q13 are associated with the metastatic characteristics of H4M cells. The amplification of cyclin D1 gene CCND1 in H4M cells may be the cause of the amplification of chromosome 11q13.
背景和目的:肝細胞癌(hepatocellularcarcinoma,HCC)預后差的原因是術后復發率高和早期通過門靜脈轉移。本研究建立門靜脈癌栓肝癌細胞株,并探討其分子細胞遺傳特征。方法:從HCC患者門脈癌栓中獲取肝癌細胞進行細胞培養,對培養成功的細胞(H4M)進行染色體G帶染色后分析其核型和對比基因組雜交(comparativegenomichybridization,CGH)結果;用差異PCR檢測周期素D1基因。結果:H4M細胞核型為超3倍體,染色體數為71~78,其中有一條標記染色體含有一長的均染區(hsr)。H4M主要的細胞遺傳學改變為8p缺失和11q13高拷貝數擴增。周期素D1基因CCND1明顯擴增。結論:染色體8p丟失和11q13高拷貝數擴增與H4M細胞的轉移特性相關。周期素D1基因CCND1擴增可能是染色體11q13擴增的原因。
Persistent Identifierhttp://hdl.handle.net/10722/150793
ISSN
2015 Impact Factor: 2.814
2015 SCImago Journal Rankings: 1.081

 

DC FieldValueLanguage
dc.contributor.authorZhang, Men_US
dc.contributor.authorWen, JMen_US
dc.contributor.authorXu, JMen_US
dc.contributor.authorWang, WSen_US
dc.contributor.authorHu, Len_US
dc.contributor.authorXie, Den_US
dc.contributor.authorGuan, XYen_US
dc.date.accessioned2012-06-26T06:10:36Z-
dc.date.available2012-06-26T06:10:36Z-
dc.date.issued2002en_US
dc.identifier.citationChinese Journal of Cancer, 2002, v. 21 n. 11, p. 1203-1207en_US
dc.identifier.citation癌症, 2002, v. 21 n. 11, p. 1203-1207-
dc.identifier.issn1000-467Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/150793-
dc.description.abstractBACKGROUND & OBJECTIVE: Poor prognosis of hepatocellular carcinoma (HCC) is due to its high recurrent rate after operation and early metastasis through portal vein. This study was designed to establish a metastatic HCC cell line and to investigate its molecular and cytogenetic characterization. METHODS: Cell culture was performed using the tissue obtained from a metastatic lesion in portal vein of a patient with HCC. After G-banding staining, karyotype, and comparative genomic hybridization (CGH) of the cultured H4M cells were characterized and cyclin D1 gene CCND1 was detected by differential PCR. RESULTS: The karyotype of metastatic HCC cell strain is a hypertriploid (71-78 chromosomes) with a huge marker chromosome containing a long homogeneously staining region (hsr). The main genetic alterations in H4M cells were a high copy number amplification of 11q13 and loss of 8p. Cyclin D1 gene CCND1 was also amplified significantly in the cells. CONCLUSIONS: The loss of chromosome 8p and high copy number amplification of 11q13 are associated with the metastatic characteristics of H4M cells. The amplification of cyclin D1 gene CCND1 in H4M cells may be the cause of the amplification of chromosome 11q13.en_US
dc.description.abstract背景和目的:肝細胞癌(hepatocellularcarcinoma,HCC)預后差的原因是術后復發率高和早期通過門靜脈轉移。本研究建立門靜脈癌栓肝癌細胞株,并探討其分子細胞遺傳特征。方法:從HCC患者門脈癌栓中獲取肝癌細胞進行細胞培養,對培養成功的細胞(H4M)進行染色體G帶染色后分析其核型和對比基因組雜交(comparativegenomichybridization,CGH)結果;用差異PCR檢測周期素D1基因。結果:H4M細胞核型為超3倍體,染色體數為71~78,其中有一條標記染色體含有一長的均染區(hsr)。H4M主要的細胞遺傳學改變為8p缺失和11q13高拷貝數擴增。周期素D1基因CCND1明顯擴增。結論:染色體8p丟失和11q13高拷貝數擴增與H4M細胞的轉移特性相關。周期素D1基因CCND1擴增可能是染色體11q13擴增的原因。-
dc.languagechien_US
dc.publisherSun Yat-sen University Cancer Center (中山醫科大學腫瘤防治中心). The Journal's web site is located at http://www.cjcsysu.cn/-
dc.relation.ispartofChinese Journal of Canceren_US
dc.relation.ispartof癌症-
dc.subject.meshAneuploidyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarcinoma, Hepatocellular - Genetics - Metabolism - Secondaryen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshChromosome Deletionen_US
dc.subject.meshChromosomes, Human, Pair 11en_US
dc.subject.meshChromosomes, Human, Pair 8en_US
dc.subject.meshCyclin D1 - Genetics - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshKaryotypingen_US
dc.subject.meshLiver Neoplasms - Genetics - Metabolism - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshMice, Sciden_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPortal Vein - Pathologyen_US
dc.titleEstablishment and cytogenetic characterization of a human hepatocellular carcinoma cell strain from the embolus in portal veinen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12526216-
dc.identifier.scopuseid_2-s2.0-21244477235en_US
dc.identifier.volume21en_US
dc.identifier.issue11en_US
dc.identifier.spage1203en_US
dc.identifier.epage1207en_US
dc.publisher.placeChina (中國)-
dc.identifier.scopusauthoridZhang, M=16302423500en_US
dc.identifier.scopusauthoridWen, JM=7402701931en_US
dc.identifier.scopusauthoridXu, JM=8850249000en_US
dc.identifier.scopusauthoridWang, WS=36488756900en_US
dc.identifier.scopusauthoridHu, L=34770075600en_US
dc.identifier.scopusauthoridXie, D=35070710200en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.customcontrol.immutablecsl 150112-

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