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Article: Amplification of 19q13.1-q13.2 sequences in ovarian cancer: G-band, FISH, and molecular studies

TitleAmplification of 19q13.1-q13.2 sequences in ovarian cancer: G-band, FISH, and molecular studies
Authors
Issue Date1996
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
Citation
Cancer Genetics And Cytogenetics, 1996, v. 87 n. 1, p. 55-62 How to Cite?
AbstractIn this study of ovarian carcinoma, we extended previous findings by performing FISH using chromosome 19 paint and microFISH probes and patient samples with and without abnormalities of chromosome 19 identified by G- bonding. Karyotype interpretations of der(19) were confirmed, while additional 19 translocations were also detected by FISH with 19WCP in some cases. Similar FISH studies of ovarian carcinoma cell lines found chromosome 19 abnormalities even after extensive in vitro culture. MicroFISH probes were generated by chromosome microdissection from two cases with hsr(19) and mapped to 19q13.2 and 19q13.1-.2, respectively. FISH with these microFISH probes alone or in combination with a 19 WCP probe to four patient samples and seven cell lines showed that 65% of chromosome 19 structural abnormalities contained 19q13.1-q13.2 sequences, sometimes as large hsrs. Ovarian cancer cell lines showed amplification and overexpression of the AKT2 putative oncogene, but not the ERCC-2 DNA repair gene in this chromosomal region. In addition to AKT2, amplification and overexpression of other yet- unidentified genes in the 19q13.1-q13.2 region may contribute to ovarian carcinoma pathogenesis or progression.
Persistent Identifierhttp://hdl.handle.net/10722/150790
ISSN
2012 Impact Factor: 1.929
2013 SCImago Journal Rankings: 0.872
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorThompson, FHen_US
dc.contributor.authorNelson, MAen_US
dc.contributor.authorTrent, JMen_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorYang, JMen_US
dc.contributor.authorEmerson, Jen_US
dc.contributor.authorAdair, Len_US
dc.contributor.authorWymer, Jen_US
dc.contributor.authorBalfour, Cen_US
dc.contributor.authorMassey, Ken_US
dc.contributor.authorWeinstein, Ren_US
dc.contributor.authorAlberts, DSen_US
dc.contributor.authorTaetle, Ren_US
dc.date.accessioned2012-06-26T06:10:32Z-
dc.date.available2012-06-26T06:10:32Z-
dc.date.issued1996en_US
dc.identifier.citationCancer Genetics And Cytogenetics, 1996, v. 87 n. 1, p. 55-62en_US
dc.identifier.issn0165-4608en_US
dc.identifier.urihttp://hdl.handle.net/10722/150790-
dc.description.abstractIn this study of ovarian carcinoma, we extended previous findings by performing FISH using chromosome 19 paint and microFISH probes and patient samples with and without abnormalities of chromosome 19 identified by G- bonding. Karyotype interpretations of der(19) were confirmed, while additional 19 translocations were also detected by FISH with 19WCP in some cases. Similar FISH studies of ovarian carcinoma cell lines found chromosome 19 abnormalities even after extensive in vitro culture. MicroFISH probes were generated by chromosome microdissection from two cases with hsr(19) and mapped to 19q13.2 and 19q13.1-.2, respectively. FISH with these microFISH probes alone or in combination with a 19 WCP probe to four patient samples and seven cell lines showed that 65% of chromosome 19 structural abnormalities contained 19q13.1-q13.2 sequences, sometimes as large hsrs. Ovarian cancer cell lines showed amplification and overexpression of the AKT2 putative oncogene, but not the ERCC-2 DNA repair gene in this chromosomal region. In addition to AKT2, amplification and overexpression of other yet- unidentified genes in the 19q13.1-q13.2 region may contribute to ovarian carcinoma pathogenesis or progression.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergeneen_US
dc.relation.ispartofCancer Genetics and Cytogeneticsen_US
dc.subject.meshChromosome Aberrationsen_US
dc.subject.meshChromosomes, Human, Pair 19en_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshOncogene Proteins - Geneticsen_US
dc.subject.meshOvarian Neoplasms - Geneticsen_US
dc.subject.meshProtein-Serine-Threonine Kinases - Geneticsen_US
dc.subject.meshProto-Oncogene Proteinsen_US
dc.subject.meshProto-Oncogene Proteins C-Akten_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleAmplification of 19q13.1-q13.2 sequences in ovarian cancer: G-band, FISH, and molecular studiesen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0165-4608(95)00248-0en_US
dc.identifier.pmid8646743-
dc.identifier.scopuseid_2-s2.0-17444437679en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-17444437679&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume87en_US
dc.identifier.issue1en_US
dc.identifier.spage55en_US
dc.identifier.epage62en_US
dc.identifier.isiWOS:A1996UD43100013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridThompson, FH=7202217465en_US
dc.identifier.scopusauthoridNelson, MA=7403461044en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridLiu, Y=36071856600en_US
dc.identifier.scopusauthoridYang, JM=12445064900en_US
dc.identifier.scopusauthoridEmerson, J=35919442400en_US
dc.identifier.scopusauthoridAdair, L=7005651925en_US
dc.identifier.scopusauthoridWymer, J=8665972800en_US
dc.identifier.scopusauthoridBalfour, C=6603925650en_US
dc.identifier.scopusauthoridMassey, K=7005902657en_US
dc.identifier.scopusauthoridWeinstein, R=7202286020en_US
dc.identifier.scopusauthoridAlberts, DS=35380854000en_US
dc.identifier.scopusauthoridTaetle, R=7006711648en_US

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