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Article: Generation of a complete set of human telomeric band painting probes by chromosome microdissection

TitleGeneration of a complete set of human telomeric band painting probes by chromosome microdissection
Authors
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 2004, v. 83 n. 2, p. 298-302 How to Cite?
AbstractChromosomal rearrangements involving telomeric bands have been frequently detected in many malignancies and congenital diseases. To develop a useful tool to study chromosomal rearrangements within the telomeric band effectively and accurately, a whole set of telomeric band painting probes (TBP) has been generated by chromosome microdissection. The intensity and specificity of these TBPs have been tested by fluorescence in situ hybridization and all TBPs showed strong and specific signals to target regions. TBPs of 6q and 17p were successfully used to detect the loss of the terminal band of 6q in a hepatocellular carcinoma cell line and a complex translocation involving the 17p terminal band in a melanoma cell line. Meanwhile, the TBP of 21q was used to detect a de novo translocation, t(12;21), and the breakpoint at 21q was located at 21q22.2. Further application of these TBPs should greatly facilitate the cytogenetic analysis of complex chromosome rearrangements involving telomeric bands. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/150781
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, Len_US
dc.contributor.authorSham, JSTen_US
dc.contributor.authorTjia, WMen_US
dc.contributor.authorTan, YQen_US
dc.contributor.authorLu, GXen_US
dc.contributor.authorGuan, XYen_US
dc.date.accessioned2012-06-26T06:10:25Z-
dc.date.available2012-06-26T06:10:25Z-
dc.date.issued2004en_US
dc.identifier.citationGenomics, 2004, v. 83 n. 2, p. 298-302en_US
dc.identifier.issn0888-7543en_US
dc.identifier.urihttp://hdl.handle.net/10722/150781-
dc.description.abstractChromosomal rearrangements involving telomeric bands have been frequently detected in many malignancies and congenital diseases. To develop a useful tool to study chromosomal rearrangements within the telomeric band effectively and accurately, a whole set of telomeric band painting probes (TBP) has been generated by chromosome microdissection. The intensity and specificity of these TBPs have been tested by fluorescence in situ hybridization and all TBPs showed strong and specific signals to target regions. TBPs of 6q and 17p were successfully used to detect the loss of the terminal band of 6q in a hepatocellular carcinoma cell line and a complex translocation involving the 17p terminal band in a melanoma cell line. Meanwhile, the TBP of 21q was used to detect a de novo translocation, t(12;21), and the breakpoint at 21q was located at 21q22.2. Further application of these TBPs should greatly facilitate the cytogenetic analysis of complex chromosome rearrangements involving telomeric bands. © 2003 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_US
dc.relation.ispartofGenomicsen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshChromosome Banding - Methodsen_US
dc.subject.meshChromosome Breakageen_US
dc.subject.meshChromosomes, Human, Pair 12en_US
dc.subject.meshChromosomes, Human, Pair 17en_US
dc.subject.meshChromosomes, Human, Pair 21en_US
dc.subject.meshChromosomes, Human, Pair 6en_US
dc.subject.meshDna Probesen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshTelomere - Geneticsen_US
dc.subject.meshTranslocation, Geneticen_US
dc.titleGeneration of a complete set of human telomeric band painting probes by chromosome microdissectionen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ygeno.2003.08.021en_US
dc.identifier.pmid14706458-
dc.identifier.scopuseid_2-s2.0-0346098051en_US
dc.identifier.hkuros96118-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0346098051&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume83en_US
dc.identifier.issue2en_US
dc.identifier.spage298en_US
dc.identifier.epage302en_US
dc.identifier.isiWOS:000188211400011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHu, L=34770075600en_US
dc.identifier.scopusauthoridSham, JST=7101655565en_US
dc.identifier.scopusauthoridTjia, WM=8631854600en_US
dc.identifier.scopusauthoridTan, YQ=7402139346en_US
dc.identifier.scopusauthoridLu, GX=7403460237en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US

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