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Article: Evaluation of hypermethylated tumor suppressor genes as tumor markers in mouth and throat rinsing fluid, nasopharyngeal swab and peripheral blood of nasopharygeal carcinoma patient

TitleEvaluation of hypermethylated tumor suppressor genes as tumor markers in mouth and throat rinsing fluid, nasopharyngeal swab and peripheral blood of nasopharygeal carcinoma patient
Authors
KeywordsM & T rinsing fluid
Nasopharyngeal carcinoma
Nasopharyngeal swab
Promoter hypermethylation
Tumor suppressor gene
Issue Date2003
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2003, v. 105 n. 6, p. 851-855 How to Cite?
AbstractThe purpose of our study was to evaluate the frequency of hypermethylated tumor suppressor genes (TSGs) in peripheral blood, mouth and throat (M&T) rinsing fluid and nasopharyngeal (NP) swabs of nasopharyngeal carcinoma (NPC) patients. Six normal NP tissues, 43 M&T rinsing fluid, 37 NP swabs and 43 peripheral blood from healthy non-smokers and non-drinkers without a family history of NPC, and 30 NPC tumors and their matched body fluid were analyzed for the presence of hypermethylated p15, p16, Ras association domain family 1 (RASSF1A), E-cadherin, and death-associated protein kinase (DAPK) by methylation-specific PCR. Sequencing analysis was carried out on selected NPC tumors and body fluid samples. Twenty-nine (97%) tumors displayed methylation in at least 1 of the 5 genes. The methylation frequencies were 80% for p15, 77% for DAPK, 67% for RASSF1A, 53% for E-cadherin and 33% for p16. The frequency range of aberrant methylated genes in the body fluids were NP swabs (17-63%) and M&T rinsing fluid (17-50%). Methylation was found in <20% of peripheral blood for each respective gene. Methylation was, however, detected in 1 M&T rinsing fluid in which the primary tumor showed methylation free for RASSF1A. Five healthy individuals exhibited methylation for DAPK, or RASSF1A, or p15 in their body fluid samples. All body fluid samples of healthy controls showed methylation free for E-cadherin and p16. Epigenetic change is found frequently in NPC and the high detection rate in body fluids suggest its potential application in non-invasive screening of NPC or detection of residual carcinoma after treatment. © 2003 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/150777
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References
Errata

 

DC FieldValueLanguage
dc.contributor.authorChang, HWen_HK
dc.contributor.authorChan, Aen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorWei, WIen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorYuen, APWen_HK
dc.date.accessioned2012-06-26T06:10:18Z-
dc.date.available2012-06-26T06:10:18Z-
dc.date.issued2003en_HK
dc.identifier.citationInternational Journal Of Cancer, 2003, v. 105 n. 6, p. 851-855en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/150777-
dc.description.abstractThe purpose of our study was to evaluate the frequency of hypermethylated tumor suppressor genes (TSGs) in peripheral blood, mouth and throat (M&T) rinsing fluid and nasopharyngeal (NP) swabs of nasopharyngeal carcinoma (NPC) patients. Six normal NP tissues, 43 M&T rinsing fluid, 37 NP swabs and 43 peripheral blood from healthy non-smokers and non-drinkers without a family history of NPC, and 30 NPC tumors and their matched body fluid were analyzed for the presence of hypermethylated p15, p16, Ras association domain family 1 (RASSF1A), E-cadherin, and death-associated protein kinase (DAPK) by methylation-specific PCR. Sequencing analysis was carried out on selected NPC tumors and body fluid samples. Twenty-nine (97%) tumors displayed methylation in at least 1 of the 5 genes. The methylation frequencies were 80% for p15, 77% for DAPK, 67% for RASSF1A, 53% for E-cadherin and 33% for p16. The frequency range of aberrant methylated genes in the body fluids were NP swabs (17-63%) and M&T rinsing fluid (17-50%). Methylation was found in <20% of peripheral blood for each respective gene. Methylation was, however, detected in 1 M&T rinsing fluid in which the primary tumor showed methylation free for RASSF1A. Five healthy individuals exhibited methylation for DAPK, or RASSF1A, or p15 in their body fluid samples. All body fluid samples of healthy controls showed methylation free for E-cadherin and p16. Epigenetic change is found frequently in NPC and the high detection rate in body fluids suggest its potential application in non-invasive screening of NPC or detection of residual carcinoma after treatment. © 2003 Wiley-Liss, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.subjectM & T rinsing fluiden_HK
dc.subjectNasopharyngeal carcinomaen_HK
dc.subjectNasopharyngeal swaben_HK
dc.subjectPromoter hypermethylationen_HK
dc.subjectTumor suppressor geneen_HK
dc.subject.meshAdulten_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBiopsyen_US
dc.subject.meshBody Fluids - Chemistryen_US
dc.subject.meshCarcinoma - Diagnosis - Pathologyen_US
dc.subject.meshDna Methylationen_US
dc.subject.meshDna, Neoplasm - Analysis - Blood - Chemistryen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, Tumor Suppressoren_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMouthen_US
dc.subject.meshMouthwashesen_US
dc.subject.meshNasopharyngeal Neoplasms - Diagnosis - Pathologyen_US
dc.subject.meshNasopharynx - Anatomy & Histology - Pathologyen_US
dc.subject.meshPharynxen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshTumor Markers, Biological - Analysis - Blooden_US
dc.titleEvaluation of hypermethylated tumor suppressor genes as tumor markers in mouth and throat rinsing fluid, nasopharyngeal swab and peripheral blood of nasopharygeal carcinoma patienten_HK
dc.typeArticleen_HK
dc.identifier.emailKwong, DLW: dlwkwong@hku.hken_HK
dc.identifier.emailWei, WI: hrmswwi@hku.hken_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityWei, WI=rp00323en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1002/ijc.11162en_HK
dc.identifier.pmid12767073-
dc.identifier.scopuseid_2-s2.0-0037780890en_HK
dc.identifier.hkuros88440-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037780890&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume105en_HK
dc.identifier.issue6en_HK
dc.identifier.spage851en_HK
dc.identifier.epage855en_HK
dc.identifier.isiWOS:000183515200017-
dc.publisher.placeUnited Statesen_HK
dc.relation.erratumdoi:10.1002/ijc.11438-
dc.identifier.scopusauthoridChang, HW=7407523249en_HK
dc.identifier.scopusauthoridChan, A=37019601600en_HK
dc.identifier.scopusauthoridKwong, DLW=15744231600en_HK
dc.identifier.scopusauthoridWei, WI=7403321552en_HK
dc.identifier.scopusauthoridSuam, JST=7101655565en_HK
dc.identifier.scopusauthoridYuen, APW=7006290111en_HK

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