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Article: Analysis of comparative genomic hybridization and loss of heterozygosity in 43 primary gastric carcinomas

TitleAnalysis of comparative genomic hybridization and loss of heterozygosity in 43 primary gastric carcinomas
Authors
Issue Date2003
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2003, v. 116 n. 4, p. 517-523 How to Cite?
AbstractObjective. To investigate common chromosomal changes and the LOH frequency of microsatellite loci in primary gastric cancer samples in order to locate the deleted regions in which human gastric cancer related genes might exist. Methods. Comparative genomic hybridization (CGH) was used to define global chromosomal aberrations in 43 primary gastric tumors. Based on the results of CGH, analysis of loss of heterozygosity (LOH) was performed in chromosome 19 in which the loss was first discovered in the gastric cancers. The PCR-based approach was used to investigate 22 loci, which are spaced at 1. 1 - 10. 9 cM intervals throughout chromosome 19. The amplified PCR fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan™ and Genotyper™. Results. CGH analysis revealed gains in chromosome 3p(8/43), 8q(8/43), 20 [20 (9/43), 20p (7/43), 20q(4/43) ], 12q(16/43), 13q(12/43) and losses in 19 [19 (15/43) ], 7 [17 (8/43), 17p (10/43) ], 16 (10/43) and 1 p (11/43). Among the 43 evaluated samples, the most frequent LOH was detected at locus D19S571 (27.81%). Conclusions. The tumorigenesis of gastric cancer includes several chromosomal changes. The aberration of chromosome 19 was the first common change founded in gastric cancer. The region near the D19S571 might harbor potential genes related to the tumorigenesis of gastric cancer.
Persistent Identifierhttp://hdl.handle.net/10722/150776
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Qen_US
dc.contributor.authorWang, Ben_US
dc.contributor.authorGuan, Xen_US
dc.contributor.authorGao, Hen_US
dc.contributor.authorCheng, Hen_US
dc.contributor.authorZhang, Qen_US
dc.contributor.authorHuang, Cen_US
dc.contributor.authorLi, Pen_US
dc.contributor.authorFu, Sen_US
dc.date.accessioned2012-06-26T06:10:12Z-
dc.date.available2012-06-26T06:10:12Z-
dc.date.issued2003en_US
dc.identifier.citationChinese Medical Journal, 2003, v. 116 n. 4, p. 517-523en_US
dc.identifier.issn0366-6999en_US
dc.identifier.urihttp://hdl.handle.net/10722/150776-
dc.description.abstractObjective. To investigate common chromosomal changes and the LOH frequency of microsatellite loci in primary gastric cancer samples in order to locate the deleted regions in which human gastric cancer related genes might exist. Methods. Comparative genomic hybridization (CGH) was used to define global chromosomal aberrations in 43 primary gastric tumors. Based on the results of CGH, analysis of loss of heterozygosity (LOH) was performed in chromosome 19 in which the loss was first discovered in the gastric cancers. The PCR-based approach was used to investigate 22 loci, which are spaced at 1. 1 - 10. 9 cM intervals throughout chromosome 19. The amplified PCR fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan™ and Genotyper™. Results. CGH analysis revealed gains in chromosome 3p(8/43), 8q(8/43), 20 [20 (9/43), 20p (7/43), 20q(4/43) ], 12q(16/43), 13q(12/43) and losses in 19 [19 (15/43) ], 7 [17 (8/43), 17p (10/43) ], 16 (10/43) and 1 p (11/43). Among the 43 evaluated samples, the most frequent LOH was detected at locus D19S571 (27.81%). Conclusions. The tumorigenesis of gastric cancer includes several chromosomal changes. The aberration of chromosome 19 was the first common change founded in gastric cancer. The region near the D19S571 might harbor potential genes related to the tumorigenesis of gastric cancer.en_US
dc.languageengen_US
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_US
dc.relation.ispartofChinese Medical Journalen_US
dc.subject.meshChromosomes, Human, Pair 19en_US
dc.subject.meshHumansen_US
dc.subject.meshLoss Of Heterozygosityen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPeutz-Jeghers Syndrome - Geneticsen_US
dc.subject.meshStomach Neoplasms - Geneticsen_US
dc.titleAnalysis of comparative genomic hybridization and loss of heterozygosity in 43 primary gastric carcinomasen_US
dc.typeArticleen_US
dc.identifier.emailGuan, X:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, X=rp00454en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid12875714-
dc.identifier.scopuseid_2-s2.0-0037606053en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037606053&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume116en_US
dc.identifier.issue4en_US
dc.identifier.spage517en_US
dc.identifier.epage523en_US
dc.identifier.isiWOS:000183404200008-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWang, Q=35306290000en_US
dc.identifier.scopusauthoridWang, B=9043707000en_US
dc.identifier.scopusauthoridGuan, X=7201463221en_US
dc.identifier.scopusauthoridGao, H=55205273000en_US
dc.identifier.scopusauthoridCheng, H=36770304200en_US
dc.identifier.scopusauthoridZhang, Q=7407966946en_US
dc.identifier.scopusauthoridHuang, C=7406888918en_US
dc.identifier.scopusauthoridLi, P=7404774183en_US
dc.identifier.scopusauthoridFu, S=7402732199en_US

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