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Article: A rat cell line derived from DMBA-induced mammary carcinoma

TitleA rat cell line derived from DMBA-induced mammary carcinoma
Authors
Issue Date2003
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2003, v. 73 n. 1, p. 27-40 How to Cite?
AbstractA new cell line, designated UHKBR-01, was successfully established from a 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat mammary tumour. DMBA was administered orally at a dose of 4 mg/ml per rat on the first day of the experiment and thereafter at weekly intervals of same dosage, until the rats have reached a weight of around 150-200 g. The tumours grew rapidly after the injection, and were transplanted into nude mice one the harvest size (2.5×2×1 mm3) was reached, it was transplanted onto nude mice. We have developed a cell line from a portion of the DMBA-induced carcinoma of the nude mice. The UHKBR-01 cell exhibited a slow increase in growth rate during the time of culture and was highly tumourigenic in nude mice. The cells have been grown in culture for over 40 passages. Characterization of the cell line was performed. This included morphology by light and transmission electron microscopy, karyotype, growth rate, tumour antigen expression and xenograft implantation into nude mice. These cells exhibit ultrastructural and immunohistochemical features of epithelial cells of mammary origin. The above analyses also demonstrated that UHKBR-01 cells were oestrogen- and progesterone-receptor positive, in likeness to other established breast cancer cell lines such as MDA-MB-231 and MCF-7. The cell line grows as monolayers of oval-shaped cells with large folded nuclei accompanied by a rich supply of mitochondria. This report describes the first in vitro cell line from transplantable DMBA-induced mammary carcinoma of nude mice, which presents unique characteristics that may prove to be a good experimental model for investigating breast cancer biology. © 2003 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/150774
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChow, LWCen_US
dc.contributor.authorCheung, MNBen_US
dc.contributor.authorLoo, WTYen_US
dc.contributor.authorGuan, XYen_US
dc.date.accessioned2012-06-26T06:10:10Z-
dc.date.available2012-06-26T06:10:10Z-
dc.date.issued2003en_US
dc.identifier.citationLife Sciences, 2003, v. 73 n. 1, p. 27-40en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/150774-
dc.description.abstractA new cell line, designated UHKBR-01, was successfully established from a 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat mammary tumour. DMBA was administered orally at a dose of 4 mg/ml per rat on the first day of the experiment and thereafter at weekly intervals of same dosage, until the rats have reached a weight of around 150-200 g. The tumours grew rapidly after the injection, and were transplanted into nude mice one the harvest size (2.5×2×1 mm3) was reached, it was transplanted onto nude mice. We have developed a cell line from a portion of the DMBA-induced carcinoma of the nude mice. The UHKBR-01 cell exhibited a slow increase in growth rate during the time of culture and was highly tumourigenic in nude mice. The cells have been grown in culture for over 40 passages. Characterization of the cell line was performed. This included morphology by light and transmission electron microscopy, karyotype, growth rate, tumour antigen expression and xenograft implantation into nude mice. These cells exhibit ultrastructural and immunohistochemical features of epithelial cells of mammary origin. The above analyses also demonstrated that UHKBR-01 cells were oestrogen- and progesterone-receptor positive, in likeness to other established breast cancer cell lines such as MDA-MB-231 and MCF-7. The cell line grows as monolayers of oval-shaped cells with large folded nuclei accompanied by a rich supply of mitochondria. This report describes the first in vitro cell line from transplantable DMBA-induced mammary carcinoma of nude mice, which presents unique characteristics that may prove to be a good experimental model for investigating breast cancer biology. © 2003 Elsevier Science Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.subject.mesh9,10-Dimethyl-1,2-Benzanthracene - Toxicityen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Neoplasm - Analysisen_US
dc.subject.meshAntineoplastic Agents - Pharmacologyen_US
dc.subject.meshCarcinogens - Toxicityen_US
dc.subject.meshFemaleen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshKaryotypingen_US
dc.subject.meshMammary Neoplasms, Experimental - Chemically Induced - Drug Therapy - Pathologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshNeoplasm Transplantationen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleA rat cell line derived from DMBA-induced mammary carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0024-3205(03)00253-4en_US
dc.identifier.pmid12726884-
dc.identifier.scopuseid_2-s2.0-0037462169en_US
dc.identifier.hkuros77018-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037462169&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume73en_US
dc.identifier.issue1en_US
dc.identifier.spage27en_US
dc.identifier.epage40en_US
dc.identifier.isiWOS:000182775600003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChow, LWC=7202532995en_US
dc.identifier.scopusauthoridCheung, MNB=7201897548en_US
dc.identifier.scopusauthoridLoo, WTY=7003567474en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US

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