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Article: Detection of hypermethylated RIZ1 gene in primary tumor, mouth, and throat rinsing fluid, nasopharyngeal swab, and peripheral blood of nasopharyngeal carcinoma patient

TitleDetection of hypermethylated RIZ1 gene in primary tumor, mouth, and throat rinsing fluid, nasopharyngeal swab, and peripheral blood of nasopharyngeal carcinoma patient
Authors
Issue Date2003
Citation
Clinical Cancer Research, 2003, v. 9 n. 3, p. 1033-1038 How to Cite?
AbstractPurpose: The aims of this study are to examine the methylation status of the Fn2 RIZ1 gene in nasopharyngeal (NP) tumors and nasopharyngeal carcinoma (NPC) cell lines and to evaluate the clinical value of methylated RIZ1 gene in body fluid samples of NPC patients. Experimental design: Methylation status of RIZ1 was evaluated by MSP on CNE-2 and M1 cell lines, 30 tumor biopsies, and their matched body fluid samples, including mouth and throat (M & T) rinsing fluid, NP swabs, plasma, and buffy coat. Normal controls included 8 normal NP biopsies and body fluid samples from 29 healthy volunteers. Sequencing was performed on MSP products from one NP tumor and one M & T rinsing fluid. Transcription of the RIZ1 gene before and after 5-aza-2′-deoxycytidine treatment was examined on CNE-2. Results: The methylated RIZ1 gene was detected in both CNE-2 and M1, 18 (60%) NP tumors, but not in any of the normal controls. Of 30 matched body fluid samples, methylated RIZ1 DNA was found in 11 (37%) NP swabs, 9 (30%) M & T rinsing fluid, 7 (23%) plasma, and 3 (10%) buffy coat samples. Sequencing analysis confirmed all cytosines to uracils conversion, excluding cytosines in CpG dinucleotides in methylated PCR products. Promoter methylation correlated with loss of RIZ1 mRNA expression, and 5-aza-2′-deoxycytidine treatment restored its expression in CNE-2. Conclusion: Our results suggest that promoter hypermethylation of the RIZ1 gene is commonly found in NPC. Its detection in body fluid samples of NPC patients but not in normal controls indicates that it is worth to further evaluate its clinical application in assisting screening of NPC and monitoring recurrence after treatment.
Persistent Identifierhttp://hdl.handle.net/10722/150773
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChang, HWen_HK
dc.contributor.authorChan, Aen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorWei, WIen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorYuen, APWen_HK
dc.date.accessioned2012-06-26T06:10:10Z-
dc.date.available2012-06-26T06:10:10Z-
dc.date.issued2003en_HK
dc.identifier.citationClinical Cancer Research, 2003, v. 9 n. 3, p. 1033-1038en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/150773-
dc.description.abstractPurpose: The aims of this study are to examine the methylation status of the Fn2 RIZ1 gene in nasopharyngeal (NP) tumors and nasopharyngeal carcinoma (NPC) cell lines and to evaluate the clinical value of methylated RIZ1 gene in body fluid samples of NPC patients. Experimental design: Methylation status of RIZ1 was evaluated by MSP on CNE-2 and M1 cell lines, 30 tumor biopsies, and their matched body fluid samples, including mouth and throat (M & T) rinsing fluid, NP swabs, plasma, and buffy coat. Normal controls included 8 normal NP biopsies and body fluid samples from 29 healthy volunteers. Sequencing was performed on MSP products from one NP tumor and one M & T rinsing fluid. Transcription of the RIZ1 gene before and after 5-aza-2′-deoxycytidine treatment was examined on CNE-2. Results: The methylated RIZ1 gene was detected in both CNE-2 and M1, 18 (60%) NP tumors, but not in any of the normal controls. Of 30 matched body fluid samples, methylated RIZ1 DNA was found in 11 (37%) NP swabs, 9 (30%) M & T rinsing fluid, 7 (23%) plasma, and 3 (10%) buffy coat samples. Sequencing analysis confirmed all cytosines to uracils conversion, excluding cytosines in CpG dinucleotides in methylated PCR products. Promoter methylation correlated with loss of RIZ1 mRNA expression, and 5-aza-2′-deoxycytidine treatment restored its expression in CNE-2. Conclusion: Our results suggest that promoter hypermethylation of the RIZ1 gene is commonly found in NPC. Its detection in body fluid samples of NPC patients but not in normal controls indicates that it is worth to further evaluate its clinical application in assisting screening of NPC and monitoring recurrence after treatment.en_HK
dc.languageengen_US
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subject.meshAzacitidine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshCarcinoma - Blood - Genetics - Metabolismen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshDna - Metabolismen_US
dc.subject.meshDna Methylationen_US
dc.subject.meshDna-Binding Proteins - Geneticsen_US
dc.subject.meshHistone-Lysine N-Methyltransferaseen_US
dc.subject.meshHumansen_US
dc.subject.meshMouth - Metabolismen_US
dc.subject.meshNasopharyngeal Neoplasms - Blood - Genetics - Metabolismen_US
dc.subject.meshNuclear Proteins - Geneticsen_US
dc.subject.meshPharynx - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSulfites - Pharmacologyen_US
dc.subject.meshTranscription Factors - Geneticsen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleDetection of hypermethylated RIZ1 gene in primary tumor, mouth, and throat rinsing fluid, nasopharyngeal swab, and peripheral blood of nasopharyngeal carcinoma patienten_HK
dc.typeArticleen_HK
dc.identifier.emailKwong, DLW: dlwkwong@hku.hken_HK
dc.identifier.emailWei, WI: hrmswwi@hku.hken_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityWei, WI=rp00323en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid12631603-
dc.identifier.scopuseid_2-s2.0-0037343180en_HK
dc.identifier.hkuros88688-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037343180&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1033en_HK
dc.identifier.epage1038en_HK
dc.identifier.isiWOS:000181430400016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChang, HW=7407523249en_HK
dc.identifier.scopusauthoridChan, A=37019601600en_HK
dc.identifier.scopusauthoridKwong, DLW=15744231600en_HK
dc.identifier.scopusauthoridWei, WI=7403321552en_HK
dc.identifier.scopusauthoridSham, JST=24472255400en_HK
dc.identifier.scopusauthoridYuen, APW=7006290111en_HK

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