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Article: Anti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line

TitleAnti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line
Authors
Issue Date2002
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2002, v. 72 n. 3, p. 257-268 How to Cite?
AbstractA novel approach for the treatment of leukemia is the differentiation therapy in which immature leukemia cells are induced to attain a mature phenotype when exposed to differentiation inducers, either alone or in combinations with other chemotherapeutic or chemopreventive drugs. Over the past decade, numerous studies indicated that green tea catechins (GTC) could suppress the growth and induce apoptosis on a number of human cancer cell lines. However, the differentiation-inducing activity of GTC on human tumors remains poorly understood. In the present study, the effect of the major GTC epigallocatechin-3-gallate (EGCG) on the proliferation and differentiation of a human eosinophilc leukemic cell line, EoL-1, was examined. Our results showed that EGCG suppressed the proliferation of the EoL-1 cells in a dose-dependent manner, with an estimated IC50 value of 31.5 μM. On the other hand, EGCG at a concentration of 40 μM could trigger the EoL-1 cells to undergo morphological differentiation into mature eosinophil-like cells. Using RT-PCR and flow cytometry, it was found that EGCG upregulated the gene and protein expression of two eosinophil-specific granule proteins, the major basic protein (MBP) and eosinophil peroxidase (EPO), in EoL-1 cells. Taken together, our findings suggest that EGCG can exhibit anti-leukemic activity on a human eosinophilic cell line EoL-1 by suppressing the proliferation and by inducing the differentiation of the leukemia cells. © 2002 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/150769
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLung, HLen_US
dc.contributor.authorIp, WKen_US
dc.contributor.authorWong, CKen_US
dc.contributor.authorMak, NKen_US
dc.contributor.authorChen, ZYen_US
dc.contributor.authorLeung, KNen_US
dc.date.accessioned2012-06-26T06:10:06Z-
dc.date.available2012-06-26T06:10:06Z-
dc.date.issued2002en_US
dc.identifier.citationLife Sciences, 2002, v. 72 n. 3, p. 257-268en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/150769-
dc.description.abstractA novel approach for the treatment of leukemia is the differentiation therapy in which immature leukemia cells are induced to attain a mature phenotype when exposed to differentiation inducers, either alone or in combinations with other chemotherapeutic or chemopreventive drugs. Over the past decade, numerous studies indicated that green tea catechins (GTC) could suppress the growth and induce apoptosis on a number of human cancer cell lines. However, the differentiation-inducing activity of GTC on human tumors remains poorly understood. In the present study, the effect of the major GTC epigallocatechin-3-gallate (EGCG) on the proliferation and differentiation of a human eosinophilc leukemic cell line, EoL-1, was examined. Our results showed that EGCG suppressed the proliferation of the EoL-1 cells in a dose-dependent manner, with an estimated IC50 value of 31.5 μM. On the other hand, EGCG at a concentration of 40 μM could trigger the EoL-1 cells to undergo morphological differentiation into mature eosinophil-like cells. Using RT-PCR and flow cytometry, it was found that EGCG upregulated the gene and protein expression of two eosinophil-specific granule proteins, the major basic protein (MBP) and eosinophil peroxidase (EPO), in EoL-1 cells. Taken together, our findings suggest that EGCG can exhibit anti-leukemic activity on a human eosinophilic cell line EoL-1 by suppressing the proliferation and by inducing the differentiation of the leukemia cells. © 2002 Elsevier Science Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.subject.meshAntineoplastic Agents, Phytogenic - Pharmacologyen_US
dc.subject.meshBlood Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshCatechin - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEosinophil Granule Proteinsen_US
dc.subject.meshEosinophil Peroxidaseen_US
dc.subject.meshGrowth Inhibitors - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshHypereosinophilic Syndrome - Drug Therapy - Metabolism - Pathologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshPeroxidases - Biosynthesis - Geneticsen_US
dc.subject.meshRna, Neoplasm - Biosynthesisen_US
dc.subject.meshRibonucleasesen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleAnti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell lineen_US
dc.typeArticleen_US
dc.identifier.emailLung, HL:hllung2@hku.hken_US
dc.identifier.authorityLung, HL=rp00299en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0024-3205(02)02236-1en_US
dc.identifier.pmid12427485-
dc.identifier.scopuseid_2-s2.0-0037032621en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037032621&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume72en_US
dc.identifier.issue3en_US
dc.identifier.spage257en_US
dc.identifier.epage268en_US
dc.identifier.isiWOS:000179231100004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLung, HL=6603819904en_US
dc.identifier.scopusauthoridIp, WK=7102820063en_US
dc.identifier.scopusauthoridWong, CK=13310337300en_US
dc.identifier.scopusauthoridMak, NK=35587830100en_US
dc.identifier.scopusauthoridChen, ZY=7409487061en_US
dc.identifier.scopusauthoridLeung, KN=7401860476en_US

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