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Article: Different expression of hepatitis B surface antigen between hepatocellular carcinoma and its surrounding liver tissue, studied using a tissue microarray

TitleDifferent expression of hepatitis B surface antigen between hepatocellular carcinoma and its surrounding liver tissue, studied using a tissue microarray
Authors
Issue Date2002
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
Citation
Journal Of Pathology, 2002, v. 197 n. 5, p. 610-616 How to Cite?
AbstractHepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and is highly associated with chronic liver disease, including hepatitis B viral infection. In order to study the association between hepatitis B virus (HBV) infection and HCC development, tissue microarrays were used to detect the expression of hepatitis B surface antigen (HBsAg) in 194 HCCs and their surrounding liver tissues, using anti-HBsAg monoclonal antibody. The results showed that the expression of HBsAg is significantly lower in tumour tissue than in non-tumour tissue. Among the 138 cases with positive serum HBsAg, expression of HBsAg was more frequently detected in non-tumour tissue (103 cases, 75%) than in tumour tissue (11 cases, 8%). RT-PCR and Southern blot analysis were performed to explore the mechanism of the decreased expression of HBsAg in tumour cells. The RT-PCR results showed that absence or decreased expression of the HBV S gene was detected in 3/15 (20%) and 6/15 (40%) HCCs, respectively. Integration of HBV in 23 pairs of HCCs and their matched non-tumour liver tissues was studied by Southern blot. The results showed that the integrated HBV S gene sequence was detected in 19/23 tumours (83%) and 1/23 non-tumour tissues (4%), whereas the free replicative virus form was observed in 3/23 tumours (13%) and 14/23 non-tumour tissues (61%). These findings suggest that HBsAg-negative results in tumour tissues were directly related to HBV DNA insertion and provide new insights into the involvement of HBsAg in hepatocarcinogenesis. Copyright © 2002 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/150763
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_US
dc.contributor.authorWu, MCen_US
dc.contributor.authorSham, JSTen_US
dc.contributor.authorTai, LSen_US
dc.contributor.authorFang, Yen_US
dc.contributor.authorWu, WQen_US
dc.contributor.authorXie, Den_US
dc.contributor.authorGuan, XYen_US
dc.date.accessioned2012-06-26T06:10:01Z-
dc.date.available2012-06-26T06:10:01Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Pathology, 2002, v. 197 n. 5, p. 610-616en_US
dc.identifier.issn0022-3417en_US
dc.identifier.urihttp://hdl.handle.net/10722/150763-
dc.description.abstractHepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and is highly associated with chronic liver disease, including hepatitis B viral infection. In order to study the association between hepatitis B virus (HBV) infection and HCC development, tissue microarrays were used to detect the expression of hepatitis B surface antigen (HBsAg) in 194 HCCs and their surrounding liver tissues, using anti-HBsAg monoclonal antibody. The results showed that the expression of HBsAg is significantly lower in tumour tissue than in non-tumour tissue. Among the 138 cases with positive serum HBsAg, expression of HBsAg was more frequently detected in non-tumour tissue (103 cases, 75%) than in tumour tissue (11 cases, 8%). RT-PCR and Southern blot analysis were performed to explore the mechanism of the decreased expression of HBsAg in tumour cells. The RT-PCR results showed that absence or decreased expression of the HBV S gene was detected in 3/15 (20%) and 6/15 (40%) HCCs, respectively. Integration of HBV in 23 pairs of HCCs and their matched non-tumour liver tissues was studied by Southern blot. The results showed that the integrated HBV S gene sequence was detected in 19/23 tumours (83%) and 1/23 non-tumour tissues (4%), whereas the free replicative virus form was observed in 3/23 tumours (13%) and 14/23 non-tumour tissues (61%). These findings suggest that HBsAg-negative results in tumour tissues were directly related to HBV DNA insertion and provide new insights into the involvement of HBsAg in hepatocarcinogenesis. Copyright © 2002 John Wiley & Sons, Ltd.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130en_US
dc.relation.ispartofJournal of Pathologyen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshCarcinoma, Hepatocellular - Immunology - Virologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHepatitis B Surface Antigens - Analysisen_US
dc.subject.meshHepatitis B Virus - Isolation & Purificationen_US
dc.subject.meshHepatitis B, Chronic - Complicationsen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver - Virologyen_US
dc.subject.meshLiver Neoplasms - Immunology - Virologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshOligonucleotide Array Sequence Analysisen_US
dc.subject.meshVirus Integrationen_US
dc.titleDifferent expression of hepatitis B surface antigen between hepatocellular carcinoma and its surrounding liver tissue, studied using a tissue microarrayen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/path.1150en_US
dc.identifier.pmid12210080-
dc.identifier.scopuseid_2-s2.0-0036054630en_US
dc.identifier.hkuros80969-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036054630&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume197en_US
dc.identifier.issue5en_US
dc.identifier.spage610en_US
dc.identifier.epage616en_US
dc.identifier.isiWOS:000177504700007-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWang, Y=7601486269en_US
dc.identifier.scopusauthoridWu, MC=7405593399en_US
dc.identifier.scopusauthoridSham, JST=7101655565en_US
dc.identifier.scopusauthoridTai, LS=7004457333en_US
dc.identifier.scopusauthoridFang, Y=7403457405en_US
dc.identifier.scopusauthoridWu, WQ=8720711100en_US
dc.identifier.scopusauthoridXie, D=35070710200en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US

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