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Article: Induction of tumor necrosis factor receptor type 2 gene expression by tumor necrosis factor-α in rat primary astrocytes

TitleInduction of tumor necrosis factor receptor type 2 gene expression by tumor necrosis factor-α in rat primary astrocytes
Authors
Issue Date2001
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2001, v. 68 n. 18, p. 2081-2091 How to Cite?
AbstractUsing reverse transcription-polymerase chain reaction (RT-PCR) technique, the messenger RNA (mRNA) for tumor necrosis factor receptor type 2 (TNF-R2, 75/80 kDa) was detected in rat primary astrocytes, with much lower level of expression when compared to that for tumor necrosis factor receptor type 1 (TNF-R1, 55/60 kDa). Upon exposure to TNF-α (100 U/ml), the TNF-R2 mRNA level was greatly enhanced at 8 h, while TNF-R1 mRNA remained unchanged even after 24 h. The induction of TNF-R2 gene expression by TNF-α was dose-dependent and seemed to be unique to TNF-α, as interleukin-6 (IL-6) had no significant effect on TNF-R2 expression. Since TNF-R2 was reported to mediate mitogenic and gene-inducing effects in many other cell types, it is likely that the reported proliferative effect of TNF-α on astrocytes was also mediated by this TNF receptor subtype. Upon exposure to TNF-α or lipopolysaccharide (LPS), the expression of TNF-α gene was induced, and the LPS-induced TNF-α seemed to selectively enhance the TNF-R2 gene expression. Collectively, our results suggest that the TNF-α or LPS-induced expression of both TNF-R2 and TNF-α may provide a positive control mechanism to further enhance the proliferative effect of TNF-α in astrocytes. © 2001 Elsevier Science Inc.
Persistent Identifierhttp://hdl.handle.net/10722/150762
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLung, HLen_US
dc.contributor.authorLeung, KNen_US
dc.contributor.authorStadlin, Aen_US
dc.contributor.authorMa, CMen_US
dc.contributor.authorTsang, Den_US
dc.date.accessioned2012-06-26T06:10:00Z-
dc.date.available2012-06-26T06:10:00Z-
dc.date.issued2001en_US
dc.identifier.citationLife Sciences, 2001, v. 68 n. 18, p. 2081-2091en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/150762-
dc.description.abstractUsing reverse transcription-polymerase chain reaction (RT-PCR) technique, the messenger RNA (mRNA) for tumor necrosis factor receptor type 2 (TNF-R2, 75/80 kDa) was detected in rat primary astrocytes, with much lower level of expression when compared to that for tumor necrosis factor receptor type 1 (TNF-R1, 55/60 kDa). Upon exposure to TNF-α (100 U/ml), the TNF-R2 mRNA level was greatly enhanced at 8 h, while TNF-R1 mRNA remained unchanged even after 24 h. The induction of TNF-R2 gene expression by TNF-α was dose-dependent and seemed to be unique to TNF-α, as interleukin-6 (IL-6) had no significant effect on TNF-R2 expression. Since TNF-R2 was reported to mediate mitogenic and gene-inducing effects in many other cell types, it is likely that the reported proliferative effect of TNF-α on astrocytes was also mediated by this TNF receptor subtype. Upon exposure to TNF-α or lipopolysaccharide (LPS), the expression of TNF-α gene was induced, and the LPS-induced TNF-α seemed to selectively enhance the TNF-R2 gene expression. Collectively, our results suggest that the TNF-α or LPS-induced expression of both TNF-R2 and TNF-α may provide a positive control mechanism to further enhance the proliferative effect of TNF-α in astrocytes. © 2001 Elsevier Science Inc.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshAntigens, Cd - Biosynthesis - Geneticsen_US
dc.subject.meshAstrocytes - Drug Effects - Metabolismen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDna Primers - Chemistryen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshGene Expression - Drug Effectsen_US
dc.subject.meshInterleukin-6 - Pharmacologyen_US
dc.subject.meshLipopolysaccharides - Pharmacologyen_US
dc.subject.meshOligonucleotides, Antisense - Chemistryen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Tumor Necrosis Factor - Biosynthesis - Geneticsen_US
dc.subject.meshReceptors, Tumor Necrosis Factor, Type Ien_US
dc.subject.meshReceptors, Tumor Necrosis Factor, Type Iien_US
dc.subject.meshRecombinant Proteins - Pharmacologyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Genetics - Pharmacologyen_US
dc.titleInduction of tumor necrosis factor receptor type 2 gene expression by tumor necrosis factor-α in rat primary astrocytesen_US
dc.typeArticleen_US
dc.identifier.emailLung, HL:hllung2@hku.hken_US
dc.identifier.authorityLung, HL=rp00299en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0024-3205(01)00997-3en_US
dc.identifier.pmid11324713-
dc.identifier.scopuseid_2-s2.0-0035937423en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035937423&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume68en_US
dc.identifier.issue18en_US
dc.identifier.spage2081en_US
dc.identifier.epage2091en_US
dc.identifier.isiWOS:000167852100003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLung, HL=6603819904en_US
dc.identifier.scopusauthoridLeung, KN=7401860476en_US
dc.identifier.scopusauthoridStadlin, A=6602676624en_US
dc.identifier.scopusauthoridMa, CM=36986460800en_US
dc.identifier.scopusauthoridTsang, D=7005609135en_US

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