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Article: Expression of protein kinase C isoforms in euxanthone-induced differentiation of neuroblastoma cells

TitleExpression of protein kinase C isoforms in euxanthone-induced differentiation of neuroblastoma cells
Authors
Issue Date2001
PublisherGeorg Thieme Verlag. The Journal's web site is located at http://www.thieme.de/plantamedica/index.html
Citation
Planta Medica, 2001, v. 67 n. 5, p. 400-405 How to Cite?
AbstractEuxanthone, a potent neuritogenic compound isolated from the roots of the medicinal herb Polygala caudata, has recently been shown to induce the differentiation of murine neuroblastoma Neuro 2A (BU-1) cells. In this study, the role of protein kinase C (PKC) and the expression of various PKC isoforms in euxanthone-treated BU-1 cells were examined, mRNA phenotyping using the reverse-transcription polymerase chain reaction (RT-PCR) showed that BU-1 cells express six different PKC isoforms, namely PKC-α, -β, -δ, -ε, -λ, and -ζ, Differential regulation and expression of PKC isoforms was observed in BU-1 cells treated with 100 μM euxanthone. PKC-α, -β, -δ, -λ, and -ζ were all up-regulated, with 1.7- to 9.5-fold increase, at around 30 to 60 minutes after euxanthone treatment. The expression level of PKC-ε remained relatively constant during the treatment. PKC-γ -η, and -Θ were not detected in both untreated and euxanthone-treated BU-1 cells. Staurosporine, a broad spectrum PKC inhibitor, was found to inhibit both spontaneous and euxanthone-induced neuritogenesis in BU-1 cells. A significant reduction of the euxanthone-induced neuritogenic effect was also observed when the PKC isoform-specific inhibitor Go6976 was included in the culture. These results suggest that the euxanthone-induced differentiation of the neuroblastoma BU-1 cells may be mediated through the differential expression of PKC-α, -β, -δ, -λ and -ζ isoforms.
Persistent Identifierhttp://hdl.handle.net/10722/150758
ISSN
2015 Impact Factor: 1.99
2015 SCImago Journal Rankings: 0.702
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMak, NKen_US
dc.contributor.authorLung, HLen_US
dc.contributor.authorWong, RNSen_US
dc.contributor.authorLeung, HWen_US
dc.contributor.authorTsang, HYen_US
dc.contributor.authorLeung, KNen_US
dc.date.accessioned2012-06-26T06:09:58Z-
dc.date.available2012-06-26T06:09:58Z-
dc.date.issued2001en_US
dc.identifier.citationPlanta Medica, 2001, v. 67 n. 5, p. 400-405en_US
dc.identifier.issn0032-0943en_US
dc.identifier.urihttp://hdl.handle.net/10722/150758-
dc.description.abstractEuxanthone, a potent neuritogenic compound isolated from the roots of the medicinal herb Polygala caudata, has recently been shown to induce the differentiation of murine neuroblastoma Neuro 2A (BU-1) cells. In this study, the role of protein kinase C (PKC) and the expression of various PKC isoforms in euxanthone-treated BU-1 cells were examined, mRNA phenotyping using the reverse-transcription polymerase chain reaction (RT-PCR) showed that BU-1 cells express six different PKC isoforms, namely PKC-α, -β, -δ, -ε, -λ, and -ζ, Differential regulation and expression of PKC isoforms was observed in BU-1 cells treated with 100 μM euxanthone. PKC-α, -β, -δ, -λ, and -ζ were all up-regulated, with 1.7- to 9.5-fold increase, at around 30 to 60 minutes after euxanthone treatment. The expression level of PKC-ε remained relatively constant during the treatment. PKC-γ -η, and -Θ were not detected in both untreated and euxanthone-treated BU-1 cells. Staurosporine, a broad spectrum PKC inhibitor, was found to inhibit both spontaneous and euxanthone-induced neuritogenesis in BU-1 cells. A significant reduction of the euxanthone-induced neuritogenic effect was also observed when the PKC isoform-specific inhibitor Go6976 was included in the culture. These results suggest that the euxanthone-induced differentiation of the neuroblastoma BU-1 cells may be mediated through the differential expression of PKC-α, -β, -δ, -λ and -ζ isoforms.en_US
dc.languageengen_US
dc.publisherGeorg Thieme Verlag. The Journal's web site is located at http://www.thieme.de/plantamedica/index.htmlen_US
dc.relation.ispartofPlanta Medicaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntineoplastic Agents, Phytogenic - Chemistry - Isolation & Purification - Pharmacologyen_US
dc.subject.meshCarbazoles - Pharmacologyen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshGene Expression Regulation, Enzymologicen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshIndoles - Pharmacologyen_US
dc.subject.meshIsoenzymes - Genetics - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshNeurites - Drug Effectsen_US
dc.subject.meshNeuroblastoma - Enzymology - Ultrastructureen_US
dc.subject.meshProtein Kinase C - Antagonists & Inhibitors - Genetics - Metabolismen_US
dc.subject.meshRosales - Chemistryen_US
dc.subject.meshStaurosporine - Pharmacologyen_US
dc.subject.meshXanthenes - Chemistry - Isolation & Purification - Pharmacologyen_US
dc.subject.meshXanthonesen_US
dc.titleExpression of protein kinase C isoforms in euxanthone-induced differentiation of neuroblastoma cellsen_US
dc.typeArticleen_US
dc.identifier.emailLung, HL:hllung2@hku.hken_US
dc.identifier.authorityLung, HL=rp00299en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1055/s-2001-15809en_US
dc.identifier.pmid11488451-
dc.identifier.scopuseid_2-s2.0-0034941094en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034941094&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume67en_US
dc.identifier.issue5en_US
dc.identifier.spage400en_US
dc.identifier.epage405en_US
dc.identifier.isiWOS:000170038600003-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridMak, NK=35587830100en_US
dc.identifier.scopusauthoridLung, HL=6603819904en_US
dc.identifier.scopusauthoridWong, RNS=35270797800en_US
dc.identifier.scopusauthoridLeung, HW=7202811443en_US
dc.identifier.scopusauthoridTsang, HY=46361867300en_US
dc.identifier.scopusauthoridLeung, KN=7401860476en_US

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