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Article: Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences

TitleHuman artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences
Authors
Issue Date1999
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1999, v. 96 n. 2, p. 592-597 How to Cite?
AbstractA human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was selected based on its size from among several YACs identified by screening a randomly chosen subset of the Centre d'Etude du Polymorphisme Humain (CEPH) (Paris) YAC library with a degenerate alpha satellite probe. This YAC, which also included non-alpha satellite DNA, was modified to contain human telomeric DNA and a putative origin of replication from the human β-globin locus. The resultant HAC vector was introduced into human cells by lipid-mediated DNA transfection, and HACs were identified that bound the active kinetochore protein CENP-E and were mitotically stable in the absence of selection for at least 100 generations. Microdissected HACs used as fluorescence in situ hybridization probes localized to the HAC itself and not to the arms of any endogenous human chromosomes, suggesting that the HAC was not formed by telomere fragmentation. Our ability to manipulate the HAC vector by recombinant genetic methods should allow us to further define the elements necessary for mammalian chromosome function.
Persistent Identifierhttp://hdl.handle.net/10722/150752
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHenning, KAen_US
dc.contributor.authorNovotny, EAen_US
dc.contributor.authorCompton, STen_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorLiu, PPen_US
dc.contributor.authorAshlock, MAen_US
dc.date.accessioned2012-06-26T06:09:54Z-
dc.date.available2012-06-26T06:09:54Z-
dc.date.issued1999en_US
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1999, v. 96 n. 2, p. 592-597en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/150752-
dc.description.abstractA human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was selected based on its size from among several YACs identified by screening a randomly chosen subset of the Centre d'Etude du Polymorphisme Humain (CEPH) (Paris) YAC library with a degenerate alpha satellite probe. This YAC, which also included non-alpha satellite DNA, was modified to contain human telomeric DNA and a putative origin of replication from the human β-globin locus. The resultant HAC vector was introduced into human cells by lipid-mediated DNA transfection, and HACs were identified that bound the active kinetochore protein CENP-E and were mitotically stable in the absence of selection for at least 100 generations. Microdissected HACs used as fluorescence in situ hybridization probes localized to the HAC itself and not to the arms of any endogenous human chromosomes, suggesting that the HAC was not formed by telomere fragmentation. Our ability to manipulate the HAC vector by recombinant genetic methods should allow us to further define the elements necessary for mammalian chromosome function.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subject.meshCell Lineen_US
dc.subject.meshChromosomes - Geneticsen_US
dc.subject.meshChromosomes, Artificial, Yeast - Geneticsen_US
dc.subject.meshClone Cells - Metabolismen_US
dc.subject.meshDna Fragmentation - Geneticsen_US
dc.subject.meshDna Probes - Geneticsen_US
dc.subject.meshDna, Satellite - Geneticsen_US
dc.subject.meshGene Dosageen_US
dc.subject.meshGenetic Vectors - Geneticsen_US
dc.subject.meshGlobins - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMitosis - Geneticsen_US
dc.subject.meshReplication Origin - Geneticsen_US
dc.subject.meshTelomere - Geneticsen_US
dc.subject.meshTransfection - Geneticsen_US
dc.titleHuman artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequencesen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1073/pnas.96.2.592en_US
dc.identifier.pmid9892678-
dc.identifier.scopuseid_2-s2.0-0033582293en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033582293&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume96en_US
dc.identifier.issue2en_US
dc.identifier.spage592en_US
dc.identifier.epage597en_US
dc.identifier.isiWOS:000078189200052-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHenning, KA=35963184000en_US
dc.identifier.scopusauthoridNovotny, EA=7004406260en_US
dc.identifier.scopusauthoridCompton, ST=7102893873en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridLiu, PP=7404618192en_US
dc.identifier.scopusauthoridAshlock, MA=6603049981en_US

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