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Article: Detection of p53 mutations in Hong Kong colorectal carcinomas by conventional PCR-SSCP analysis versus p53 yeast functional assays

TitleDetection of p53 mutations in Hong Kong colorectal carcinomas by conventional PCR-SSCP analysis versus p53 yeast functional assays
Authors
Issue Date1999
Citation
Anticancer Research, 1999, v. 19 n. 1 A, p. 625-628 How to Cite?
AbstractBackground: Previous reports indicate that the p53 yeast functional assay is a highly sensitive method of detection of p53 mutations in clinical specimens. Our earlier report (1) showed a 35.4% p53 mutation frequency in Hong Kong colorectal carcinoma (CRC) patients, when conventional molecular screening techniques were utilized to assess the mutation rate in the hot spots in exons 5-8. Materials and methods: The yeast functional assay was used to determine if the previous mutation frequency determined by PCR-SSCP techniques was under-estimated, and if so, to see if other hot spots for mutations explain this difference. Results: The p53 functional yeast assay results showed an increased mutation frequency. However, sequencing showed the mutations were confined to common hot spots for mutation in exons 6 and 7. Conclusions: The mutation frequency in CRC patients observed with the yeast assay is higher than previously reported. Forty-five percent of 20 SSCP-negative specimens were positive by the yeast assay, which this study shows is superior for detection of p53 mutations directly in clinical specimens containing varying amounts of normal tissue contamination.
Persistent Identifierhttp://hdl.handle.net/10722/150745
ISSN
2015 Impact Factor: 1.895
2015 SCImago Journal Rankings: 0.815
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, CSen_US
dc.contributor.authorLung, MLen_US
dc.date.accessioned2012-06-26T06:09:42Z-
dc.date.available2012-06-26T06:09:42Z-
dc.date.issued1999en_US
dc.identifier.citationAnticancer Research, 1999, v. 19 n. 1 A, p. 625-628en_US
dc.identifier.issn0250-7005en_US
dc.identifier.urihttp://hdl.handle.net/10722/150745-
dc.description.abstractBackground: Previous reports indicate that the p53 yeast functional assay is a highly sensitive method of detection of p53 mutations in clinical specimens. Our earlier report (1) showed a 35.4% p53 mutation frequency in Hong Kong colorectal carcinoma (CRC) patients, when conventional molecular screening techniques were utilized to assess the mutation rate in the hot spots in exons 5-8. Materials and methods: The yeast functional assay was used to determine if the previous mutation frequency determined by PCR-SSCP techniques was under-estimated, and if so, to see if other hot spots for mutations explain this difference. Results: The p53 functional yeast assay results showed an increased mutation frequency. However, sequencing showed the mutations were confined to common hot spots for mutation in exons 6 and 7. Conclusions: The mutation frequency in CRC patients observed with the yeast assay is higher than previously reported. Forty-five percent of 20 SSCP-negative specimens were positive by the yeast assay, which this study shows is superior for detection of p53 mutations directly in clinical specimens containing varying amounts of normal tissue contamination.en_US
dc.languageengen_US
dc.relation.ispartofAnticancer Researchen_US
dc.subject.meshColorectal Neoplasms - Geneticsen_US
dc.subject.meshGenes, P53en_US
dc.subject.meshHumansen_US
dc.subject.meshMutationen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPolymorphism, Single-Stranded Conformationalen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshYeasts - Geneticsen_US
dc.titleDetection of p53 mutations in Hong Kong colorectal carcinomas by conventional PCR-SSCP analysis versus p53 yeast functional assaysen_US
dc.typeArticleen_US
dc.identifier.emailLung, ML:mlilung@hku.hken_US
dc.identifier.authorityLung, ML=rp00300en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10226610-
dc.identifier.scopuseid_2-s2.0-0032955781en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032955781&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume19en_US
dc.identifier.issue1 Aen_US
dc.identifier.spage625en_US
dc.identifier.epage628en_US
dc.identifier.isiWOS:000079786100094-
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridLeung, CS=36725508900en_US
dc.identifier.scopusauthoridLung, ML=7006411788en_US

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