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Article: Microdissection of pig chromosomes: Dissection of whole chromosomes, arms and bands for construction of paints and libraries

TitleMicrodissection of pig chromosomes: Dissection of whole chromosomes, arms and bands for construction of paints and libraries
Authors
Issue Date1998
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/submit.asp?ref=0018-0661
Citation
Hereditas, 1998, v. 128 n. 3, p. 265-271 How to Cite?
AbstractChromosome microdissection is an important means to efficiently generate a large number of markers from a desired region of a genome. The present study was designed to initiate microdissection and amplification of DNA from whole chromosomes, arms, or bands of porcine chromosomes. The following pig (SSC) chromosomes/segments were scraped: SSC1p, SSC1q26-q2.13, SSC2q11-q14, SSC4q12-q25, SSC13, SSC13q12-q31, SSC13q32-q43, SSC13q32-q43, SSC15, and SSC16q21-q23. After amplification and PCR-labelling, the DNA from the dissected segments were painted back to normal metaphase chromosomes to test their identity. Microdissection of some of the segments (on SSC4, 13 and 15) coincides with the mapping of economically important traits. As a first step towards generation of markers, microloning of amplified product from SSC1p and SSC15 was carried out. The libraries were screened with a (GT)15 oligonucleotideprobe. Future prospects of such a work in farm animals are discussed.
Persistent Identifierhttp://hdl.handle.net/10722/150736
ISSN
2015 Impact Factor: 1.083
2015 SCImago Journal Rankings: 0.423
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChaudhary, Ren_US
dc.contributor.authorKijas, Jen_US
dc.contributor.authorRaudsepp, Ten_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorZhang, Hen_US
dc.contributor.authorChowdhary, BPen_US
dc.date.accessioned2012-06-26T06:09:26Z-
dc.date.available2012-06-26T06:09:26Z-
dc.date.issued1998en_US
dc.identifier.citationHereditas, 1998, v. 128 n. 3, p. 265-271en_US
dc.identifier.issn0018-0661en_US
dc.identifier.urihttp://hdl.handle.net/10722/150736-
dc.description.abstractChromosome microdissection is an important means to efficiently generate a large number of markers from a desired region of a genome. The present study was designed to initiate microdissection and amplification of DNA from whole chromosomes, arms, or bands of porcine chromosomes. The following pig (SSC) chromosomes/segments were scraped: SSC1p, SSC1q26-q2.13, SSC2q11-q14, SSC4q12-q25, SSC13, SSC13q12-q31, SSC13q32-q43, SSC13q32-q43, SSC15, and SSC16q21-q23. After amplification and PCR-labelling, the DNA from the dissected segments were painted back to normal metaphase chromosomes to test their identity. Microdissection of some of the segments (on SSC4, 13 and 15) coincides with the mapping of economically important traits. As a first step towards generation of markers, microloning of amplified product from SSC1p and SSC15 was carried out. The libraries were screened with a (GT)15 oligonucleotideprobe. Future prospects of such a work in farm animals are discussed.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/submit.asp?ref=0018-0661en_US
dc.relation.ispartofHereditasen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshGene Libraryen_US
dc.subject.meshGenetic Techniquesen_US
dc.subject.meshGenomeen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSwine - Geneticsen_US
dc.titleMicrodissection of pig chromosomes: Dissection of whole chromosomes, arms and bands for construction of paints and librariesen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9760875-
dc.identifier.scopuseid_2-s2.0-0031670345en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031670345&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume128en_US
dc.identifier.issue3en_US
dc.identifier.spage265en_US
dc.identifier.epage271en_US
dc.identifier.isiWOS:000075709500012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChaudhary, R=7102392882en_US
dc.identifier.scopusauthoridKijas, J=6603723515en_US
dc.identifier.scopusauthoridRaudsepp, T=6603846859en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridZhang, H=7409194743en_US
dc.identifier.scopusauthoridChowdhary, BP=7006769243en_US

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