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Article: Steroidogenic factor-1 is an essential transcriptional activator for gonad-specific expression of promoter I of the rat prolactin receptor gene

TitleSteroidogenic factor-1 is an essential transcriptional activator for gonad-specific expression of promoter I of the rat prolactin receptor gene
Authors
Issue Date1997
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1997, v. 272 n. 22, p. 14263-14271 How to Cite?
AbstractThe expression of the prolactin receptor is under the control of two putative tissue-specific (PI, gonads; PII, liver) and one common (PIII) promoters (Hu, Z. Z., Zhuang, L., and Dufau, M. L. (1996) J. Biol. Chem. 271, 10242-10246). The three promoter regions were co-localized to the rat chromosomal locus 2q16, in the order 5'-PIII-PI-PII-3'. To investigate the mechanisms of gonad-specific utilization of PI, the promoter domain, regulatory cis-elements, and trans-factors were identified in gonadal cells. The promoter domain localized to the 152-base pair 5' of the transcriptional start site at -549 is highly active in gonadal cells but has minimal activity in hepatoma cells. It contains a steroidogenic factor 1 (SF-1) element (- 668) that binds the SF-1 protein of nuclear extracts from gonadal cells and is essential for promoter activation. A CCAAT box (-623) contributes minimally to basal activity in the absence of the SF-1 element, and two adjacent TATA-like sequences act as inhibitory elements. Thus, PI belongs to a class of TATA-less/non-initiator gene promoters. These findings demonstrate an essential role for SF-1 in transcriptional activation of promoter I of the prolactin receptor gene, which may explain the tissue-specific expression of PI in the gonads but not in the liver and the mammary gland.
Persistent Identifierhttp://hdl.handle.net/10722/150732
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, Zen_US
dc.contributor.authorZhuang, Len_US
dc.contributor.authorGuan, Xen_US
dc.contributor.authorMeng, Jen_US
dc.contributor.authorDufau, MLen_US
dc.date.accessioned2012-06-26T06:09:22Z-
dc.date.available2012-06-26T06:09:22Z-
dc.date.issued1997en_US
dc.identifier.citationJournal Of Biological Chemistry, 1997, v. 272 n. 22, p. 14263-14271en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/150732-
dc.description.abstractThe expression of the prolactin receptor is under the control of two putative tissue-specific (PI, gonads; PII, liver) and one common (PIII) promoters (Hu, Z. Z., Zhuang, L., and Dufau, M. L. (1996) J. Biol. Chem. 271, 10242-10246). The three promoter regions were co-localized to the rat chromosomal locus 2q16, in the order 5'-PIII-PI-PII-3'. To investigate the mechanisms of gonad-specific utilization of PI, the promoter domain, regulatory cis-elements, and trans-factors were identified in gonadal cells. The promoter domain localized to the 152-base pair 5' of the transcriptional start site at -549 is highly active in gonadal cells but has minimal activity in hepatoma cells. It contains a steroidogenic factor 1 (SF-1) element (- 668) that binds the SF-1 protein of nuclear extracts from gonadal cells and is essential for promoter activation. A CCAAT box (-623) contributes minimally to basal activity in the absence of the SF-1 element, and two adjacent TATA-like sequences act as inhibitory elements. Thus, PI belongs to a class of TATA-less/non-initiator gene promoters. These findings demonstrate an essential role for SF-1 in transcriptional activation of promoter I of the prolactin receptor gene, which may explain the tissue-specific expression of PI in the gonads but not in the liver and the mammary gland.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshDna-Binding Proteins - Genetics - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFushi Tarazu Transcription Factorsen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHomeodomain Proteinsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshOrgan Specificityen_US
dc.subject.meshOvary - Metabolismen_US
dc.subject.meshPromoter Regions, Genetic - Geneticsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Cytoplasmic And Nuclearen_US
dc.subject.meshReceptors, Prolactin - Genetics - Metabolismen_US
dc.subject.meshSteroidogenic Factor 1en_US
dc.subject.meshTestis - Metabolismen_US
dc.subject.meshTrans-Activators - Genetics - Metabolismen_US
dc.subject.meshTranscription Factors - Genetics - Metabolismen_US
dc.titleSteroidogenic factor-1 is an essential transcriptional activator for gonad-specific expression of promoter I of the rat prolactin receptor geneen_US
dc.typeArticleen_US
dc.identifier.emailGuan, X:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, X=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.272.22.14263en_US
dc.identifier.pmid9162060-
dc.identifier.scopuseid_2-s2.0-0030968863en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030968863&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume272en_US
dc.identifier.issue22en_US
dc.identifier.spage14263en_US
dc.identifier.epage14271en_US
dc.identifier.isiWOS:A1997XB49200047-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHu, Z=8084701100en_US
dc.identifier.scopusauthoridZhuang, L=7102368247en_US
dc.identifier.scopusauthoridGuan, X=7201463221en_US
dc.identifier.scopusauthoridMeng, J=7202449758en_US
dc.identifier.scopusauthoridDufau, ML=7102279466en_US

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