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Article: Isolation of a cosmid sublibrary for a region of chromosome 12 frequently amplified in human cancers using a complex chromosome microdissection probe

TitleIsolation of a cosmid sublibrary for a region of chromosome 12 frequently amplified in human cancers using a complex chromosome microdissection probe
Authors
Issue Date1996
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 1996, v. 31 n. 3, p. 343-347 How to Cite?
AbstractChromosome-specific cosmid libraries are an extremely useful resource for positional cloning projects. Once a particular region of interest has been identified, it would be of value to have an approach for isolating chromosome band-specific cosmids that could be assembled into a sublibrary for rapid screening. We constructed a region-specific sublibrary of 700 cosmids by screening a chromosome 12-specific cosmid library with a complex probe generated by degenerate oligonucleotide-primed PCR of a microdissected homogeneously staining region containing sequences amplified from chromosome 12q13-q15. Based on fluorescence in situ hybridization, approximately 60% of the cosmids in the sublibrary were derived from the microdissected region. To demonstrate further the utility of this sublibrary, a 150-kb contig containing the SAS and CDK4 genes was constructed, as well as several additional contigs between CDK4 and MDM2. This study demonstrates the possibility of utilizing probes generated by microdissection for assembling band-specific sublibraries that are amenable to rapid screening with multiple markers.
Persistent Identifierhttp://hdl.handle.net/10722/150724
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorElkahloun, AGen_US
dc.contributor.authorMeltzer, PSen_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorMcninch, JSen_US
dc.contributor.authorTrent, JMen_US
dc.contributor.authorDe Jong, PJen_US
dc.date.accessioned2012-06-26T06:09:14Z-
dc.date.available2012-06-26T06:09:14Z-
dc.date.issued1996en_US
dc.identifier.citationGenomics, 1996, v. 31 n. 3, p. 343-347en_US
dc.identifier.issn0888-7543en_US
dc.identifier.urihttp://hdl.handle.net/10722/150724-
dc.description.abstractChromosome-specific cosmid libraries are an extremely useful resource for positional cloning projects. Once a particular region of interest has been identified, it would be of value to have an approach for isolating chromosome band-specific cosmids that could be assembled into a sublibrary for rapid screening. We constructed a region-specific sublibrary of 700 cosmids by screening a chromosome 12-specific cosmid library with a complex probe generated by degenerate oligonucleotide-primed PCR of a microdissected homogeneously staining region containing sequences amplified from chromosome 12q13-q15. Based on fluorescence in situ hybridization, approximately 60% of the cosmids in the sublibrary were derived from the microdissected region. To demonstrate further the utility of this sublibrary, a 150-kb contig containing the SAS and CDK4 genes was constructed, as well as several additional contigs between CDK4 and MDM2. This study demonstrates the possibility of utilizing probes generated by microdissection for assembling band-specific sublibraries that are amenable to rapid screening with multiple markers.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_US
dc.relation.ispartofGenomicsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshChromosomes, Human, Pair 12en_US
dc.subject.meshCosmidsen_US
dc.subject.meshCyclin-Dependent Kinase 4en_US
dc.subject.meshCyclin-Dependent Kinases - Geneticsen_US
dc.subject.meshDna Primersen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshGenomic Libraryen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeoplasms - Geneticsen_US
dc.subject.meshProto-Oncogene Proteinsen_US
dc.titleIsolation of a cosmid sublibrary for a region of chromosome 12 frequently amplified in human cancers using a complex chromosome microdissection probeen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/geno.1996.0057en_US
dc.identifier.pmid8838316-
dc.identifier.scopuseid_2-s2.0-0030044806en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030044806&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume31en_US
dc.identifier.issue3en_US
dc.identifier.spage343en_US
dc.identifier.epage347en_US
dc.identifier.isiWOS:A1996TU59200010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridElkahloun, AG=6604000257en_US
dc.identifier.scopusauthoridMeltzer, PS=7102464641en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridMcninch, JS=6701785042en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US
dc.identifier.scopusauthoridDe Jong, PJ=7402783473en_US

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