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Article: Localization by chromosome microdissection of a recurrent breakpoint region on chromosome 6 in human B-cell lymphoma

TitleLocalization by chromosome microdissection of a recurrent breakpoint region on chromosome 6 in human B-cell lymphoma
Authors
Issue Date1996
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 1996, v. 88 n. 4, p. 1418-1422 How to Cite?
AbstractDeletion of the long arm of chromosome 6 (6q) is one of the most common chromosomal alterations in human B-cell lymphomas. Conventional cytogenetic banding analysis and loss-of-heterozygosity (LOH) studies have detected several common regions of deletion ranging across the entire long arm (6q), with no defined recurrent breakpoint yet identified. We describe here a strategy combining chromosome microdissection and fluorescence in situ hybridization (Micro-FISH) to determine a minimal region of deletion along chromosome 6. Seven clinical cases and one cell line of follicular lymphoma containing a t(14;18) and one case of diffuse lymphoma, also with a t(14;18), were used for this study. All nine cases had previously defined abnormalities of chromosome 6 determined by cytogenetic analysis. The results of chromosome dissection were unexpected and in contrast to the suggestion of disparate breakpoints by conventional chromosome banding. Specifically, Micro-FiSH analysis provided evidence for a common breakpoint at 6q11 in seven of nine cases. After Micro-FISH analysis, all of the presumed simple deletions of chromosome 6 were carefully reanalyzed and shown to actually represent either nonreciprocal translocations (three cases), interstitial deletions (five cases), or isochromosome (one case). The recurrent proximal breakpoint (6q11) was detected in seven of nine cases, with the minimal region of deletion encompassing 6q11 to 6q21. By analogy to other tumor systems, the identification of recurring breakpoints within 6q11 may suggest that a gene(s) important to the genesis or progression of follicular lymphoma can be localized to this band region.
Persistent Identifierhttp://hdl.handle.net/10722/150722
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuan, XYen_US
dc.contributor.authorHorsman, Den_US
dc.contributor.authorZhang, HEen_US
dc.contributor.authorParsa, NZen_US
dc.contributor.authorMeltzer, PSen_US
dc.contributor.authorTrent, JMen_US
dc.date.accessioned2012-06-26T06:09:13Z-
dc.date.available2012-06-26T06:09:13Z-
dc.date.issued1996en_US
dc.identifier.citationBlood, 1996, v. 88 n. 4, p. 1418-1422en_US
dc.identifier.issn0006-4971en_US
dc.identifier.urihttp://hdl.handle.net/10722/150722-
dc.description.abstractDeletion of the long arm of chromosome 6 (6q) is one of the most common chromosomal alterations in human B-cell lymphomas. Conventional cytogenetic banding analysis and loss-of-heterozygosity (LOH) studies have detected several common regions of deletion ranging across the entire long arm (6q), with no defined recurrent breakpoint yet identified. We describe here a strategy combining chromosome microdissection and fluorescence in situ hybridization (Micro-FISH) to determine a minimal region of deletion along chromosome 6. Seven clinical cases and one cell line of follicular lymphoma containing a t(14;18) and one case of diffuse lymphoma, also with a t(14;18), were used for this study. All nine cases had previously defined abnormalities of chromosome 6 determined by cytogenetic analysis. The results of chromosome dissection were unexpected and in contrast to the suggestion of disparate breakpoints by conventional chromosome banding. Specifically, Micro-FiSH analysis provided evidence for a common breakpoint at 6q11 in seven of nine cases. After Micro-FISH analysis, all of the presumed simple deletions of chromosome 6 were carefully reanalyzed and shown to actually represent either nonreciprocal translocations (three cases), interstitial deletions (five cases), or isochromosome (one case). The recurrent proximal breakpoint (6q11) was detected in seven of nine cases, with the minimal region of deletion encompassing 6q11 to 6q21. By analogy to other tumor systems, the identification of recurring breakpoints within 6q11 may suggest that a gene(s) important to the genesis or progression of follicular lymphoma can be localized to this band region.en_US
dc.languageengen_US
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_US
dc.relation.ispartofBlooden_US
dc.subject.meshChromosome Aberrations - Geneticsen_US
dc.subject.meshChromosome Disordersen_US
dc.subject.meshChromosome Mapping - Methodsen_US
dc.subject.meshChromosomes, Human, Pair 6en_US
dc.subject.meshDna, Neoplasm - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescence - Methodsen_US
dc.subject.meshLymphoma, B-Cell - Geneticsen_US
dc.subject.meshSequence Deletionen_US
dc.subject.meshTranslocation, Genetic - Geneticsen_US
dc.titleLocalization by chromosome microdissection of a recurrent breakpoint region on chromosome 6 in human B-cell lymphomaen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8695862-
dc.identifier.scopuseid_2-s2.0-0029822222en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029822222&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume88en_US
dc.identifier.issue4en_US
dc.identifier.spage1418en_US
dc.identifier.epage1422en_US
dc.identifier.isiWOS:A1996VC85400033-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridHorsman, D=7006139607en_US
dc.identifier.scopusauthoridZhang, HE=7409194743en_US
dc.identifier.scopusauthoridParsa, NZ=6701854941en_US
dc.identifier.scopusauthoridMeltzer, PS=7102464641en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US

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