File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Hybrid selection of transcribed sequences from microdissected DNA: Isolation of genes within an amplified region at 20q11-q13.2 in breast cancer

TitleHybrid selection of transcribed sequences from microdissected DNA: Isolation of genes within an amplified region at 20q11-q13.2 in breast cancer
Authors
Issue Date1996
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 1996, v. 56 n. 15, p. 3446-3450 How to Cite?
AbstractIn human breast carcinomas, increased copy number of DNA sequences derived from the long arm of chromosome 20 (20q) has been commonly observed by both chromosome microdissection and comparative genomic hybridization. This chromosomal region is likely to contain one or more genes that are the biological targets of this amplification event. We describe here the utilization of a chromosome microdissection-hybrid selection strategy to isolate transcribed sequences from microdissected homogeneously staining regions encompassing 20q. Using this strategy, we have isolated three novel amplified genes (termed AIB1, AIB3, and AIB4) from a cDNA library constructed from the 20q amplified breast cancer cell line BT-474. These three genes were mapped to 20q11 (AIB3 and AIB4) and 20q12 (AIB1) by fluorescence in situ hybridization. Our results indicate an unsuspected complexity to the amplification pattern of 20q in breast cancer and provide probes that will be useful for further characterization of tumor specimens.
Persistent Identifierhttp://hdl.handle.net/10722/150718
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuan, XYen_US
dc.contributor.authorXu, Jen_US
dc.contributor.authorAnzick, SLen_US
dc.contributor.authorZhang, Hen_US
dc.contributor.authorTrent, JMen_US
dc.contributor.authorMeltzer, PSen_US
dc.date.accessioned2012-06-26T06:09:10Z-
dc.date.available2012-06-26T06:09:10Z-
dc.date.issued1996en_US
dc.identifier.citationCancer Research, 1996, v. 56 n. 15, p. 3446-3450en_US
dc.identifier.issn0008-5472en_US
dc.identifier.urihttp://hdl.handle.net/10722/150718-
dc.description.abstractIn human breast carcinomas, increased copy number of DNA sequences derived from the long arm of chromosome 20 (20q) has been commonly observed by both chromosome microdissection and comparative genomic hybridization. This chromosomal region is likely to contain one or more genes that are the biological targets of this amplification event. We describe here the utilization of a chromosome microdissection-hybrid selection strategy to isolate transcribed sequences from microdissected homogeneously staining regions encompassing 20q. Using this strategy, we have isolated three novel amplified genes (termed AIB1, AIB3, and AIB4) from a cDNA library constructed from the 20q amplified breast cancer cell line BT-474. These three genes were mapped to 20q11 (AIB3 and AIB4) and 20q12 (AIB1) by fluorescence in situ hybridization. Our results indicate an unsuspected complexity to the amplification pattern of 20q in breast cancer and provide probes that will be useful for further characterization of tumor specimens.en_US
dc.languageengen_US
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_US
dc.relation.ispartofCancer Researchen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBreast Neoplasms - Geneticsen_US
dc.subject.meshChromosomes, Human, Pair 20en_US
dc.subject.meshDna, Neoplasm - Analysis - Genetics - Isolation & Purificationen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleHybrid selection of transcribed sequences from microdissected DNA: Isolation of genes within an amplified region at 20q11-q13.2 in breast canceren_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8758910-
dc.identifier.scopuseid_2-s2.0-0029680381en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029680381&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume56en_US
dc.identifier.issue15en_US
dc.identifier.spage3446en_US
dc.identifier.epage3450en_US
dc.identifier.isiWOS:A1996UZ28800013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridXu, J=7407005993en_US
dc.identifier.scopusauthoridAnzick, SL=6603376140en_US
dc.identifier.scopusauthoridZhang, H=7409194743en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US
dc.identifier.scopusauthoridMeltzer, PS=7102464641en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats