File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Coverage of chromosome 6 by chromosome microdissection: Generation of 14 subregion specific probes

TitleCoverage of chromosome 6 by chromosome microdissection: Generation of 14 subregion specific probes
Authors
Issue Date1995
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00439/index.htm
Citation
Human Genetics, 1995, v. 95 n. 6, p. 637-640 How to Cite?
AbstractHuman chromosome 6 has been subdivided by chromosome microdissection into 14 unique regions. Following microdissection, polymerase chain reaction (PCR) amplification of dissected DNA was performed using a universal primer to generate subregion-specific probes that provided complete coverage of chromosome 6. All 16 microdissections have been regionally assigned along chromosome 6 by fluorescence in situ hybridization (FISH) using biotin-labeled dissected DNA hybridized to G-banded normal metaphase chromosomes. These probes can be used as region-specific paints to generate unique 'bar codes' and for analysis of chromosome alterations involving chromosome 6 that are unidentifiable by conventional banding analysis.
Persistent Identifierhttp://hdl.handle.net/10722/150716
ISSN
2015 Impact Factor: 5.138
2015 SCImago Journal Rankings: 2.931
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGuan, XYen_US
dc.contributor.authorMeltzer, PSen_US
dc.contributor.authorBurgess, ACen_US
dc.contributor.authorTrent, JMen_US
dc.date.accessioned2012-06-26T06:09:08Z-
dc.date.available2012-06-26T06:09:08Z-
dc.date.issued1995en_US
dc.identifier.citationHuman Genetics, 1995, v. 95 n. 6, p. 637-640en_US
dc.identifier.issn0340-6717en_US
dc.identifier.urihttp://hdl.handle.net/10722/150716-
dc.description.abstractHuman chromosome 6 has been subdivided by chromosome microdissection into 14 unique regions. Following microdissection, polymerase chain reaction (PCR) amplification of dissected DNA was performed using a universal primer to generate subregion-specific probes that provided complete coverage of chromosome 6. All 16 microdissections have been regionally assigned along chromosome 6 by fluorescence in situ hybridization (FISH) using biotin-labeled dissected DNA hybridized to G-banded normal metaphase chromosomes. These probes can be used as region-specific paints to generate unique 'bar codes' and for analysis of chromosome alterations involving chromosome 6 that are unidentifiable by conventional banding analysis.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00439/index.htmen_US
dc.relation.ispartofHuman Geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromosome Bandingen_US
dc.subject.meshChromosome Mapping - Methodsen_US
dc.subject.meshChromosomes, Human, Pair 6 - Geneticsen_US
dc.subject.meshDna Probes - Geneticsen_US
dc.subject.meshDissectionen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshKaryotypingen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.titleCoverage of chromosome 6 by chromosome microdissection: Generation of 14 subregion specific probesen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7789947-
dc.identifier.scopuseid_2-s2.0-0029005783en_US
dc.identifier.volume95en_US
dc.identifier.issue6en_US
dc.identifier.spage637en_US
dc.identifier.epage640en_US
dc.identifier.isiWOS:A1995QZ62900004-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridMeltzer, PS=7102464641en_US
dc.identifier.scopusauthoridBurgess, AC=7201495330en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats