File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: DNA sequence amplification in human prostate cancer identified by chromosome microdissection: Potential prognostic implications

TitleDNA sequence amplification in human prostate cancer identified by chromosome microdissection: Potential prognostic implications
Authors
Issue Date1995
Citation
Clinical Cancer Research, 1995, v. 1 n. 1, p. 11-18 How to Cite?
AbstractThe primary aim of this report was to examine the significance of increased DNA sequence copy number (gene amplification) in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence in situ hybridization) were combined to (a) identify a common region of gene amplification in human prostate cells and (b) evaluate in patient samples the prevalence of this genetic change in both primary and recurrent prostate samples. The results of chromosome microdissection revealed a common amplified band region (8q24,1-24,2) in two prostate cases with cytological evidence of gene amplification (double minutes). Fluorescence in situ hybridization using the 8q microdissection probe was performed on fresh tumor touch preparations from 44 randomly selected prostatectomy specimens. Amplification of DNA sequences from 8q24 was observed in 4 (9%) of 44 cases. Four of the 44 patients in this series presented with a positive lymph node at initial diagnosis and 3 of these 4 patients showed 8q amplification. Because of this finding, comparative genomic hybridization and fluorescence in situ hybridization were performed on tumor cells from nine prostate cancer patients with recurrent disease. In eight of nine cases a gain of DNA sequences encompassing 8q24 was observed. Taken together with other evidence implicating 8q gain in prostate cancer progression, these results suggest that the analysis of this genetic change may have diagnostic utility as a marker of prostate cancer progression.
Persistent Identifierhttp://hdl.handle.net/10722/150715
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorVan Den Berg, Cen_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorVon Hoff, Den_US
dc.contributor.authorJenkins, Ren_US
dc.contributor.authorBittner, Men_US
dc.contributor.authorGriffin, Cen_US
dc.contributor.authorKallioniemi, Oen_US
dc.contributor.authorVisakorpi, Ten_US
dc.contributor.authorMcgill, Jen_US
dc.contributor.authorHerath, Jen_US
dc.contributor.authorEpstein, Jen_US
dc.contributor.authorSarosdy, Men_US
dc.contributor.authorMeltzer, Pen_US
dc.contributor.authorTrent, Jen_US
dc.date.accessioned2012-06-26T06:09:08Z-
dc.date.available2012-06-26T06:09:08Z-
dc.date.issued1995en_US
dc.identifier.citationClinical Cancer Research, 1995, v. 1 n. 1, p. 11-18en_US
dc.identifier.issn1078-0432en_US
dc.identifier.urihttp://hdl.handle.net/10722/150715-
dc.description.abstractThe primary aim of this report was to examine the significance of increased DNA sequence copy number (gene amplification) in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence in situ hybridization) were combined to (a) identify a common region of gene amplification in human prostate cells and (b) evaluate in patient samples the prevalence of this genetic change in both primary and recurrent prostate samples. The results of chromosome microdissection revealed a common amplified band region (8q24,1-24,2) in two prostate cases with cytological evidence of gene amplification (double minutes). Fluorescence in situ hybridization using the 8q microdissection probe was performed on fresh tumor touch preparations from 44 randomly selected prostatectomy specimens. Amplification of DNA sequences from 8q24 was observed in 4 (9%) of 44 cases. Four of the 44 patients in this series presented with a positive lymph node at initial diagnosis and 3 of these 4 patients showed 8q amplification. Because of this finding, comparative genomic hybridization and fluorescence in situ hybridization were performed on tumor cells from nine prostate cancer patients with recurrent disease. In eight of nine cases a gain of DNA sequences encompassing 8q24 was observed. Taken together with other evidence implicating 8q gain in prostate cancer progression, these results suggest that the analysis of this genetic change may have diagnostic utility as a marker of prostate cancer progression.en_US
dc.languageengen_US
dc.relation.ispartofClinical Cancer Researchen_US
dc.subject.meshChromosome Bandingen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshChromosomes, Human, Pair 8en_US
dc.subject.meshDna, Neoplasm - Geneticsen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshLymphatic Metastasisen_US
dc.subject.meshMaleen_US
dc.subject.meshNeoplasm Stagingen_US
dc.subject.meshPloidiesen_US
dc.subject.meshProstatic Neoplasms - Genetics - Pathology - Surgeryen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleDNA sequence amplification in human prostate cancer identified by chromosome microdissection: Potential prognostic implicationsen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9815882-
dc.identifier.scopuseid_2-s2.0-0028951771en_US
dc.identifier.volume1en_US
dc.identifier.issue1en_US
dc.identifier.spage11en_US
dc.identifier.epage18en_US
dc.identifier.isiWOS:A1995RJ52300003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridVan Den Berg, C=7101982459en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridVon Hoff, D=7202051416en_US
dc.identifier.scopusauthoridJenkins, R=26643087000en_US
dc.identifier.scopusauthoridBittner, M=35371357800en_US
dc.identifier.scopusauthoridGriffin, C=7201968354en_US
dc.identifier.scopusauthoridKallioniemi, O=7005031465en_US
dc.identifier.scopusauthoridVisakorpi, T=7006392459en_US
dc.identifier.scopusauthoridMcGill, J=7101993026en_US
dc.identifier.scopusauthoridHerath, J=6701432816en_US
dc.identifier.scopusauthoridEpstein, J=36043371100en_US
dc.identifier.scopusauthoridSarosdy, M=7004861589en_US
dc.identifier.scopusauthoridMeltzer, P=7102464641en_US
dc.identifier.scopusauthoridTrent, J=7201692482en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats