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Article: Direct isolation of genes encoded within a homogeneously staining region by chromosome microdissection

TitleDirect isolation of genes encoded within a homogeneously staining region by chromosome microdissection
Authors
Issue Date1994
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1994, v. 91 n. 19, p. 9121-9125 How to Cite?
AbstractIdentification of genes involved in recurring chromosome rearrangements has provided significant insight into the molecular basis of malignancy. We describe here a strategy combining chromosome microdissection and hybrid selection for the direct isolation of chromosome region-specific genes. We modeled this strategy by using sequences recovered from the microdissection of a homogeneously staining region to allow isolation of genes that were overexpressed and present at high copy number within the homogeneously staining region, including the direct isolation of two genes encoded within a 12q homogeneously staining region found in the osteosarcoma cell line OsA- CL. Although first applied to amplified genes, this strategy should be applicable to the isolation of cDNAs from any chromosomal region.
Persistent Identifierhttp://hdl.handle.net/10722/150711
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSu, YAen_US
dc.contributor.authorTrent, JMen_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorMeltzer, PSen_US
dc.date.accessioned2012-06-26T06:09:05Z-
dc.date.available2012-06-26T06:09:05Z-
dc.date.issued1994en_US
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1994, v. 91 n. 19, p. 9121-9125en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/150711-
dc.description.abstractIdentification of genes involved in recurring chromosome rearrangements has provided significant insight into the molecular basis of malignancy. We describe here a strategy combining chromosome microdissection and hybrid selection for the direct isolation of chromosome region-specific genes. We modeled this strategy by using sequences recovered from the microdissection of a homogeneously staining region to allow isolation of genes that were overexpressed and present at high copy number within the homogeneously staining region, including the direct isolation of two genes encoded within a 12q homogeneously staining region found in the osteosarcoma cell line OsA- CL. Although first applied to amplified genes, this strategy should be applicable to the isolation of cDNAs from any chromosomal region.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromosomes - Ultrastructureen_US
dc.subject.meshChromosomes, Human, Pair 12en_US
dc.subject.meshCloning, Molecular - Methodsen_US
dc.subject.meshDna Primers - Chemistryen_US
dc.subject.meshDna, Complementary - Geneticsen_US
dc.subject.meshDna, Neoplasm - Geneticsen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGene Libraryen_US
dc.subject.meshGenesen_US
dc.subject.meshGenomic Libraryen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOsteosarcoma - Geneticsen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.titleDirect isolation of genes encoded within a homogeneously staining region by chromosome microdissectionen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1073/pnas.91.19.9121en_US
dc.identifier.pmid8090779-
dc.identifier.scopuseid_2-s2.0-0028577893en_US
dc.identifier.volume91en_US
dc.identifier.issue19en_US
dc.identifier.spage9121en_US
dc.identifier.epage9125en_US
dc.identifier.isiWOS:A1994PG52100080-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSu, YA=36814427400en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridMeltzer, PS=7102464641en_US

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