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Article: Rapid detection, cloning and molecular cytogenetic characterisation of sequences from an MRP-encoding amplicon by chromosome microdissection

TitleRapid detection, cloning and molecular cytogenetic characterisation of sequences from an MRP-encoding amplicon by chromosome microdissection
Authors
Issue Date1994
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
Citation
British Journal Of Cancer, 1994, v. 70 n. 1, p. 85-90 How to Cite?
AbstractChromosome microdissection was utilised for the analysis of cytogenetic markers of gene amplification [homogeneously staining regions (hsrs) and double minutes (dmins)] in two doxorubicin-resistant cell lines, fibrosarcoma HT1080/DR4 and small-cell lung cancer H69AR. Microdissection products from the hsr(7)(p12p15) of HT1080/DR4 were amplified and used for fluorescent in situ hybridisation (micro-FISH) analysis of drug-sensitive HT1080, resistant HT1080/DR4 and normal lymphocytes. The results demonstrated that the hsr contains a domain of DNA amplification of complex origin including sequences derived from 16p11.2-16p13.1, 2q11.2, 7q32-7q34 and 10q22. The amplification was confirmed by converting the microdissected probe into a microclone library for probing HT1080 and HT1080/DR4 Southerns. A micro-FISH probe from normal band region 16p11-16p13 further demonstrated amplification of 16p sequences in both HT1080/DR4 and H69AR. During the course of this analysis. Cole et al. published the amplification of the MRP gene in H69AR cells, which maps to chromosome 16p13.1. Our results corroborate the finding of MRP amplification in these doxorubicin-resistant cell lines, but, importantly, they provide information on the composition of the complex amplicon contributions from four different chromosomes. This study demonstrates the potential utility of chromosome microdissection for the rapid recovery of sequences from amplified regions in drug-resistant cells.
Persistent Identifierhttp://hdl.handle.net/10722/150708
ISSN
2015 Impact Factor: 5.569
2015 SCImago Journal Rankings: 2.939
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRay, MEen_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorSlovak, MLen_US
dc.contributor.authorTrent, JMen_US
dc.contributor.authorMeltzer, PSen_US
dc.date.accessioned2012-06-26T06:09:03Z-
dc.date.available2012-06-26T06:09:03Z-
dc.date.issued1994en_US
dc.identifier.citationBritish Journal Of Cancer, 1994, v. 70 n. 1, p. 85-90en_US
dc.identifier.issn0007-0920en_US
dc.identifier.urihttp://hdl.handle.net/10722/150708-
dc.description.abstractChromosome microdissection was utilised for the analysis of cytogenetic markers of gene amplification [homogeneously staining regions (hsrs) and double minutes (dmins)] in two doxorubicin-resistant cell lines, fibrosarcoma HT1080/DR4 and small-cell lung cancer H69AR. Microdissection products from the hsr(7)(p12p15) of HT1080/DR4 were amplified and used for fluorescent in situ hybridisation (micro-FISH) analysis of drug-sensitive HT1080, resistant HT1080/DR4 and normal lymphocytes. The results demonstrated that the hsr contains a domain of DNA amplification of complex origin including sequences derived from 16p11.2-16p13.1, 2q11.2, 7q32-7q34 and 10q22. The amplification was confirmed by converting the microdissected probe into a microclone library for probing HT1080 and HT1080/DR4 Southerns. A micro-FISH probe from normal band region 16p11-16p13 further demonstrated amplification of 16p sequences in both HT1080/DR4 and H69AR. During the course of this analysis. Cole et al. published the amplification of the MRP gene in H69AR cells, which maps to chromosome 16p13.1. Our results corroborate the finding of MRP amplification in these doxorubicin-resistant cell lines, but, importantly, they provide information on the composition of the complex amplicon contributions from four different chromosomes. This study demonstrates the potential utility of chromosome microdissection for the rapid recovery of sequences from amplified regions in drug-resistant cells.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjcen_US
dc.relation.ispartofBritish Journal of Canceren_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshCarcinoma, Small Cell - Geneticsen_US
dc.subject.meshChromosome Mapping - Methodsen_US
dc.subject.meshChromosomes, Human, Pair 10en_US
dc.subject.meshChromosomes, Human, Pair 16en_US
dc.subject.meshChromosomes, Human, Pair 2en_US
dc.subject.meshChromosomes, Human, Pair 7en_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna Primersen_US
dc.subject.meshDna, Neoplasm - Geneticsen_US
dc.subject.meshDoxorubicin - Pharmacologyen_US
dc.subject.meshDrug Resistance - Geneticsen_US
dc.subject.meshFibrosarcoma - Geneticsen_US
dc.subject.meshGene Amplification - Geneticsen_US
dc.subject.meshGene Libraryen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescence - Methodsen_US
dc.subject.meshLung Neoplasms - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMultigene Familyen_US
dc.subject.meshSequence Analysis, Dna - Methodsen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleRapid detection, cloning and molecular cytogenetic characterisation of sequences from an MRP-encoding amplicon by chromosome microdissectionen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8018546-
dc.identifier.scopuseid_2-s2.0-0028180832en_US
dc.identifier.volume70en_US
dc.identifier.issue1en_US
dc.identifier.spage85en_US
dc.identifier.epage90en_US
dc.identifier.isiWOS:A1994NW11800015-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridRay, ME=7202799869en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridSlovak, ML=7005622779en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US
dc.identifier.scopusauthoridMeltzer, PS=7102464641en_US

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