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Article: Rapid generation of whole chromosome painting probes (WCPs) by chromosome microdissection

TitleRapid generation of whole chromosome painting probes (WCPs) by chromosome microdissection
Authors
Issue Date1994
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygeno
Citation
Genomics, 1994, v. 22 n. 1, p. 101-107 How to Cite?
AbstractA strategy for rapid construction of whole chromosome painting probes (WCPs) by chromosome microdissection has recently been developed. WCPs were prepared from 20 copies of each target chromosome microdissected from normal human metaphase chromosomes and then directly amplified by PCR using a universal primer. Fifteen WCPs, including chromosomes 1, 3, 6, 7, 9, 12, 13, 14, 15, 17, 19, 20, 21, 22, and X, have been generated using this strategy. The probe complexity and hybridization specificity of these WCPs have been characterized by gel electrophoresis and fluorescence in situ hybridization. Analysis of WCPs constructed by chromosome microdissection indicated that microdissected WCPs invariably provide strong and uniform signal intensity with no cytologically apparent cross-hybridization. To demonstrate the application of WCPs generated from microdissection, we have used these probes to detect complex chromosome rearrangements in a melanoma cell line, UM93- 007. Two different translocations involving three chromosomes [t(1;3;13) and t(1;7;13)] have been identified, both of which were undetectable by conventional banding analysis. Further application of these WCPs (including generation of WCPs from mouse and other species) should greatly facilitate the cytogenetic analysis of complex chromosome rearrangements.
Persistent Identifierhttp://hdl.handle.net/10722/150704
ISSN
2015 Impact Factor: 2.386
2015 SCImago Journal Rankings: 1.281
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGuan, XYen_US
dc.contributor.authorMeltzer, PSen_US
dc.contributor.authorTrent, JMen_US
dc.date.accessioned2012-06-26T06:09:01Z-
dc.date.available2012-06-26T06:09:01Z-
dc.date.issued1994en_US
dc.identifier.citationGenomics, 1994, v. 22 n. 1, p. 101-107en_US
dc.identifier.issn0888-7543en_US
dc.identifier.urihttp://hdl.handle.net/10722/150704-
dc.description.abstractA strategy for rapid construction of whole chromosome painting probes (WCPs) by chromosome microdissection has recently been developed. WCPs were prepared from 20 copies of each target chromosome microdissected from normal human metaphase chromosomes and then directly amplified by PCR using a universal primer. Fifteen WCPs, including chromosomes 1, 3, 6, 7, 9, 12, 13, 14, 15, 17, 19, 20, 21, 22, and X, have been generated using this strategy. The probe complexity and hybridization specificity of these WCPs have been characterized by gel electrophoresis and fluorescence in situ hybridization. Analysis of WCPs constructed by chromosome microdissection indicated that microdissected WCPs invariably provide strong and uniform signal intensity with no cytologically apparent cross-hybridization. To demonstrate the application of WCPs generated from microdissection, we have used these probes to detect complex chromosome rearrangements in a melanoma cell line, UM93- 007. Two different translocations involving three chromosomes [t(1;3;13) and t(1;7;13)] have been identified, both of which were undetectable by conventional banding analysis. Further application of these WCPs (including generation of WCPs from mouse and other species) should greatly facilitate the cytogenetic analysis of complex chromosome rearrangements.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygenoen_US
dc.relation.ispartofGenomicsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChromosome Aberrationsen_US
dc.subject.meshChromosomes, Human - Ultrastructureen_US
dc.subject.meshCytogeneticsen_US
dc.subject.meshDna - Genetics - Isolation & Purificationen_US
dc.subject.meshDissectionen_US
dc.subject.meshGene Rearrangementen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMolecular Probe Techniquesen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshTranslocation, Geneticen_US
dc.titleRapid generation of whole chromosome painting probes (WCPs) by chromosome microdissectionen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/geno.1994.1350en_US
dc.identifier.pmid7959755-
dc.identifier.scopuseid_2-s2.0-0027981573en_US
dc.identifier.volume22en_US
dc.identifier.issue1en_US
dc.identifier.spage101en_US
dc.identifier.epage107en_US
dc.identifier.isiWOS:A1994NW89800013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridMeltzer, PS=7102464641en_US
dc.identifier.scopusauthoridTrent, JM=7201692482en_US

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