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Article: Application of chromosome microdissection probes for elucidation of BCR- ABL fusion and variant Philadelphia chromosome translocations in chronic myelogenous leukemia

TitleApplication of chromosome microdissection probes for elucidation of BCR- ABL fusion and variant Philadelphia chromosome translocations in chronic myelogenous leukemia
Authors
Issue Date1993
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 1993, v. 81 n. 12, p. 3365-3371 How to Cite?
AbstractFluorescence in situ hybridization (FISH) has become an increasingly important method for assessing chromosome rearrangement. The reciprocal translocation constituting the Philadelphia (Ph) chromosome [t(9;22)(q34;q11)] characterizes more than 90% of patients with chronic myelogenous leukemia (CML). However, in the remaining cases the Ph chromosome (genetically characterized by the fusion of the BCR-ABL genes) is thought to arise through complex translocations that are often not readily apparent using routine chromosome-banding analysis. For this reason we have developed unique band-specific probes for two-color FISH that detect unequivocally the Ph chromosome, and its derivatives. Results of the application of these probes are illustrated by analysis of 11 cases of CML (9 of which contain 'variant' translocations). The probes were generated by chromosome microdissection and in vitro amplification of the bands involved in the Ph translocation, leading to an extremely fast and sensitive approach to identify this alteration in leukemic cell populations.
Persistent Identifierhttp://hdl.handle.net/10722/150700
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Jen_US
dc.contributor.authorMeltzer, Pen_US
dc.contributor.authorJenkins, Ren_US
dc.contributor.authorGuan, XYen_US
dc.contributor.authorTrent, Jen_US
dc.date.accessioned2012-06-26T06:08:59Z-
dc.date.available2012-06-26T06:08:59Z-
dc.date.issued1993en_US
dc.identifier.citationBlood, 1993, v. 81 n. 12, p. 3365-3371en_US
dc.identifier.issn0006-4971en_US
dc.identifier.urihttp://hdl.handle.net/10722/150700-
dc.description.abstractFluorescence in situ hybridization (FISH) has become an increasingly important method for assessing chromosome rearrangement. The reciprocal translocation constituting the Philadelphia (Ph) chromosome [t(9;22)(q34;q11)] characterizes more than 90% of patients with chronic myelogenous leukemia (CML). However, in the remaining cases the Ph chromosome (genetically characterized by the fusion of the BCR-ABL genes) is thought to arise through complex translocations that are often not readily apparent using routine chromosome-banding analysis. For this reason we have developed unique band-specific probes for two-color FISH that detect unequivocally the Ph chromosome, and its derivatives. Results of the application of these probes are illustrated by analysis of 11 cases of CML (9 of which contain 'variant' translocations). The probes were generated by chromosome microdissection and in vitro amplification of the bands involved in the Ph translocation, leading to an extremely fast and sensitive approach to identify this alteration in leukemic cell populations.en_US
dc.languageengen_US
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_US
dc.relation.ispartofBlooden_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshChromosomes, Human, Pair 22en_US
dc.subject.meshChromosomes, Human, Pair 9en_US
dc.subject.meshDna Probesen_US
dc.subject.meshFusion Proteins, Bcr-Abl - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshKaryotypingen_US
dc.subject.meshLeukemia, Myelogenous, Chronic, Bcr-Abl Positive - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhiladelphia Chromosomeen_US
dc.subject.meshTranslocation, Geneticen_US
dc.titleApplication of chromosome microdissection probes for elucidation of BCR- ABL fusion and variant Philadelphia chromosome translocations in chronic myelogenous leukemiaen_US
dc.typeArticleen_US
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8507874-
dc.identifier.scopuseid_2-s2.0-0027217674en_US
dc.identifier.volume81en_US
dc.identifier.issue12en_US
dc.identifier.spage3365en_US
dc.identifier.epage3371en_US
dc.identifier.isiWOS:A1993LG65900028-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhang, J=8539085200en_US
dc.identifier.scopusauthoridMeltzer, P=7102464641en_US
dc.identifier.scopusauthoridJenkins, R=26643087000en_US
dc.identifier.scopusauthoridGuan, XY=7201463221en_US
dc.identifier.scopusauthoridTrent, J=7201692482en_US

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