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Article: Direct detection of Epstein-Barr virus in peripheral blood and comparison of Epstein-Barr virus genotypes present in direct specimens and lymphoblastoid cell lines established from nasopharyngeal carcinoma patients and healthy carriers in Hong Kong

TitleDirect detection of Epstein-Barr virus in peripheral blood and comparison of Epstein-Barr virus genotypes present in direct specimens and lymphoblastoid cell lines established from nasopharyngeal carcinoma patients and healthy carriers in Hong Kong
Authors
Issue Date1992
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 1992, v. 52 n. 2, p. 174-177 How to Cite?
AbstractBy means of a PCR assay, EBV was demonstrated directly in peripheral blood of previously infected individuals. The virus was detected in approximately 80% of specimens from EBV-seropositive individuals, but not in cord-blood lymphocytes by this method. When virus present in peripheral blood was compared to that observed directly in NPC biopsies or throat washings, it was distinct from that seen in biopsies in 4/15 cases (27%) and from that seen in throat washes in 1/22 cases (5%). The throat-wash virus differed from the biopsy virus in 3/20 cases (15%). The prototype F virus was found in 7/10 LCLs (70%) established from NPC patients' peripheral blood, but was only detected in 2/9 specimens (22%) directly analyzed by the PCR assay. This finding suggests selective isolation of prototype F EBV in spontaneous LCLs established from NPC patients.
Persistent Identifierhttp://hdl.handle.net/10722/150697
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLung, MLen_US
dc.contributor.authorLam, WPen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorLi, Sen_US
dc.contributor.authorSham, Jen_US
dc.contributor.authorChoy, Den_US
dc.date.accessioned2012-06-26T06:08:55Z-
dc.date.available2012-06-26T06:08:55Z-
dc.date.issued1992en_US
dc.identifier.citationInternational Journal Of Cancer, 1992, v. 52 n. 2, p. 174-177en_US
dc.identifier.issn0020-7136en_US
dc.identifier.urihttp://hdl.handle.net/10722/150697-
dc.description.abstractBy means of a PCR assay, EBV was demonstrated directly in peripheral blood of previously infected individuals. The virus was detected in approximately 80% of specimens from EBV-seropositive individuals, but not in cord-blood lymphocytes by this method. When virus present in peripheral blood was compared to that observed directly in NPC biopsies or throat washings, it was distinct from that seen in biopsies in 4/15 cases (27%) and from that seen in throat washes in 1/22 cases (5%). The throat-wash virus differed from the biopsy virus in 3/20 cases (15%). The prototype F virus was found in 7/10 LCLs (70%) established from NPC patients' peripheral blood, but was only detected in 2/9 specimens (22%) directly analyzed by the PCR assay. This finding suggests selective isolation of prototype F EBV in spontaneous LCLs established from NPC patients.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_US
dc.relation.ispartofInternational Journal of Canceren_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshCarrier State - Microbiologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshDna, Viral - Analysisen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHerpesvirus 4, Human - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphocytes - Microbiologyen_US
dc.subject.meshNasopharyngeal Neoplasms - Microbiologyen_US
dc.subject.meshPharynx - Microbiologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.titleDirect detection of Epstein-Barr virus in peripheral blood and comparison of Epstein-Barr virus genotypes present in direct specimens and lymphoblastoid cell lines established from nasopharyngeal carcinoma patients and healthy carriers in Hong Kongen_US
dc.typeArticleen_US
dc.identifier.emailLung, ML:mlilung@hku.hken_US
dc.identifier.authorityLung, ML=rp00300en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ijc.2910520203en_US
dc.identifier.pmid1325949en_US
dc.identifier.scopuseid_2-s2.0-0026794043en_US
dc.identifier.volume52en_US
dc.identifier.issue2en_US
dc.identifier.spage174en_US
dc.identifier.epage177en_US
dc.identifier.isiWOS:A1992JM71300002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLung, ML=7006411788en_US
dc.identifier.scopusauthoridLam, WP=7203021904en_US
dc.identifier.scopusauthoridChan, KH=35338760600en_US
dc.identifier.scopusauthoridLi, S=7409240264en_US
dc.identifier.scopusauthoridSham, J=7101655565en_US
dc.identifier.scopusauthoridChoy, D=7102939127en_US

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