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Article: Evidence that respiratory tract is major reservoir for Epstein-Barr virus

TitleEvidence that respiratory tract is major reservoir for Epstein-Barr virus
Authors
Issue Date1985
PublisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
Citation
Lancet, 1985, v. 1 n. 8434, p. 889-892 How to Cite?
AbstractExfoliated cells harvested from bronchial washings of 53 patients with suspected bronchogenic carcinoma were tested by means of DNA dot hybridisation using the cloned large internal repeat (IR) sequence of Epstein-Barr virus (EBV) genome as a probe. 25 of these patients gave positive results. Since the patients had diseases that were not related to the virus, this finding suggests that the lower respiratory tract is a major reservoir for EBV. Attempts at cellular localisation of the virus revealed only an occasional cell which harboured the viral genome or expressed viral capsid antigens. These cells could not account for the quantity of the viral DNA detected in bronchial washings. Moreover, patients had similar profiles of serum EBV antibodies whether they were positive or negative for EBV DNA by dot hybridisation. These findings are compatible with a state of viral latency in which cells harbour a low copy number of the viral genome. Viral expression rarely occurs in these cells, which seem to elicit a minimum host immune response. If it is assumed that each latency infected cell harbours a maximum of approximately 30 EBV genomes (which is the lower limit of detection by the in-situ hybridisation method used in this study), the findings suggest that a considerable proportion of the exfoliative cells from the lower respiratory tract, of the order of 0.1-16% harbour latent EBV.
Persistent Identifierhttp://hdl.handle.net/10722/150682
ISSN
2015 Impact Factor: 44.002
2015 SCImago Journal Rankings: 14.638
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLung, MLen_US
dc.contributor.authorLam, WKen_US
dc.contributor.authorSo, SYen_US
dc.date.accessioned2012-06-26T06:08:39Z-
dc.date.available2012-06-26T06:08:39Z-
dc.date.issued1985en_US
dc.identifier.citationLancet, 1985, v. 1 n. 8434, p. 889-892en_US
dc.identifier.issn0140-6736en_US
dc.identifier.urihttp://hdl.handle.net/10722/150682-
dc.description.abstractExfoliated cells harvested from bronchial washings of 53 patients with suspected bronchogenic carcinoma were tested by means of DNA dot hybridisation using the cloned large internal repeat (IR) sequence of Epstein-Barr virus (EBV) genome as a probe. 25 of these patients gave positive results. Since the patients had diseases that were not related to the virus, this finding suggests that the lower respiratory tract is a major reservoir for EBV. Attempts at cellular localisation of the virus revealed only an occasional cell which harboured the viral genome or expressed viral capsid antigens. These cells could not account for the quantity of the viral DNA detected in bronchial washings. Moreover, patients had similar profiles of serum EBV antibodies whether they were positive or negative for EBV DNA by dot hybridisation. These findings are compatible with a state of viral latency in which cells harbour a low copy number of the viral genome. Viral expression rarely occurs in these cells, which seem to elicit a minimum host immune response. If it is assumed that each latency infected cell harbours a maximum of approximately 30 EBV genomes (which is the lower limit of detection by the in-situ hybridisation method used in this study), the findings suggest that a considerable proportion of the exfoliative cells from the lower respiratory tract, of the order of 0.1-16% harbour latent EBV.en_US
dc.languageengen_US
dc.publisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lanceten_US
dc.relation.ispartofLanceten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntigens, Viral - Analysisen_US
dc.subject.meshBronchi - Microbiologyen_US
dc.subject.meshCarcinoma, Bronchogenic - Microbiologyen_US
dc.subject.meshDna, Viral - Analysisen_US
dc.subject.meshFemaleen_US
dc.subject.meshHerpesvirus 4, Human - Genetics - Immunology - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshLung Neoplasms - Microbiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRetroviridae Infections - Microbiologyen_US
dc.titleEvidence that respiratory tract is major reservoir for Epstein-Barr virusen_US
dc.typeArticleen_US
dc.identifier.emailLung, ML:mlilung@hku.hken_US
dc.identifier.authorityLung, ML=rp00300en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2858744-
dc.identifier.scopuseid_2-s2.0-0021885370en_US
dc.identifier.volume1en_US
dc.identifier.issue8434en_US
dc.identifier.spage889en_US
dc.identifier.epage892en_US
dc.identifier.isiWOS:A1985AFM9000002-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLung, ML=7006411788en_US
dc.identifier.scopusauthoridLam, WK=7203021937en_US
dc.identifier.scopusauthoridSo, SY=7102397816en_US

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