File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Quantification and characterization of β-lactam resistance genes in 15 sewage treatment plants from East Asia and North America

TitleQuantification and characterization of β-lactam resistance genes in 15 sewage treatment plants from East Asia and North America
Authors
Keywordsβ-lactam resistance genes
Activated sludge
Phylogenetic analysis
Quantitative real-time PCR (q-PCR)
Issue Date2012
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology and Biotechnology, 2012, v. 95 n. 5, p. 1351-1358 How to Cite?
AbstractThe emerging antibiotic resistance genes in the aquatic environment have aroused public concern. As beta-lactam is the most widely used group of antibiotics, beta-lactam resistance genes were selected to investigate their distribution and diversity in the activated sludge from 15 geographically different sewage treatment plants (STPs) of China, Singapore, USA, and Canada. Specific PCR and quantitative real-time PCR (q-PCR) were used to investigate the occurrence and abundance of nine beta-lactam resistance genes. Five genes (OXA-1, OXA-2, OXA-10, ampC, and TEM-1) were detected in most of the sludge collected, while three genes (mecA, CTX-M-1, and SME) were not found in any sludge sample. The total abundances of the six detected beta-lactam resistance genes in the 15 STPs varied from 5.34 x 10(1) copies/ng DNA (ampC) to 5.49 x 10(4) copies/ng DNA (OXA-1). Overall, OXA-1 had the highest total concentration, followed by IMP and OXA-10. Noticeably, the abundances of TEM-1 in Chinese STPs were generally higher than those in the STPs of other countries, while the abundances of OXA-2 and IMP in the STPs of North America were much greater than those of East Asia. A total of 78 clones carrying beta-lactam resistance genes were randomly selected from six clone libraries for phylogenetic diversity analysis; the similarity of these cloned genes to known beta-lactam resistance genes with sequence identities ranged from 96% to 100%. Furthermore, OXA-1, ampC, and IMP were found to be more diverse than the other beta-lactam resistance genes.
Persistent Identifierhttp://hdl.handle.net/10722/150634
ISSN
2015 Impact Factor: 3.376
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, Yen_US
dc.contributor.authorZhang, Ten_US
dc.contributor.authorZhang, XXen_US
dc.contributor.authorLiang, DWen_US
dc.contributor.authorZhang, Men_US
dc.contributor.authorGao, DWen_US
dc.contributor.authorZhu, HGen_US
dc.contributor.authorHuang, QGen_US
dc.contributor.authorFang, HHPen_US
dc.date.accessioned2012-06-26T06:06:19Z-
dc.date.available2012-06-26T06:06:19Z-
dc.date.issued2012en_US
dc.identifier.citationApplied Microbiology and Biotechnology, 2012, v. 95 n. 5, p. 1351-1358en_US
dc.identifier.issn0175-7598en_US
dc.identifier.urihttp://hdl.handle.net/10722/150634-
dc.description.abstractThe emerging antibiotic resistance genes in the aquatic environment have aroused public concern. As beta-lactam is the most widely used group of antibiotics, beta-lactam resistance genes were selected to investigate their distribution and diversity in the activated sludge from 15 geographically different sewage treatment plants (STPs) of China, Singapore, USA, and Canada. Specific PCR and quantitative real-time PCR (q-PCR) were used to investigate the occurrence and abundance of nine beta-lactam resistance genes. Five genes (OXA-1, OXA-2, OXA-10, ampC, and TEM-1) were detected in most of the sludge collected, while three genes (mecA, CTX-M-1, and SME) were not found in any sludge sample. The total abundances of the six detected beta-lactam resistance genes in the 15 STPs varied from 5.34 x 10(1) copies/ng DNA (ampC) to 5.49 x 10(4) copies/ng DNA (OXA-1). Overall, OXA-1 had the highest total concentration, followed by IMP and OXA-10. Noticeably, the abundances of TEM-1 in Chinese STPs were generally higher than those in the STPs of other countries, while the abundances of OXA-2 and IMP in the STPs of North America were much greater than those of East Asia. A total of 78 clones carrying beta-lactam resistance genes were randomly selected from six clone libraries for phylogenetic diversity analysis; the similarity of these cloned genes to known beta-lactam resistance genes with sequence identities ranged from 96% to 100%. Furthermore, OXA-1, ampC, and IMP were found to be more diverse than the other beta-lactam resistance genes.en_US
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_US
dc.relation.ispartofApplied Microbiology and Biotechnologyen_US
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectβ-lactam resistance genesen_US
dc.subjectActivated sludgeen_US
dc.subjectPhylogenetic analysisen_US
dc.subjectQuantitative real-time PCR (q-PCR)en_US
dc.titleQuantification and characterization of β-lactam resistance genes in 15 sewage treatment plants from East Asia and North Americaen_US
dc.typeArticleen_US
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_US
dc.identifier.emailZhang, XX: zhangxx@hku.hk-
dc.identifier.emailFang, HHP: hrechef@hkucc.hku.hk-
dc.identifier.authorityFang, HHP=rp00115en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00253-011-3810-5en_US
dc.identifier.pmid22202968-
dc.identifier.scopuseid_2-s2.0-84875814190en_US
dc.identifier.hkuros208092-
dc.identifier.volume95-
dc.identifier.issue5-
dc.identifier.spage1351en_US
dc.identifier.epage1358en_US
dc.identifier.eissn1432-0614-
dc.identifier.isiWOS:000307509800023-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridYang, Y=54791748400en_US
dc.identifier.scopusauthoridZhang, T=8895885100en_US
dc.identifier.scopusauthoridZhang, XX=35239161000en_US
dc.identifier.scopusauthoridLiang, DW=15835235400en_US
dc.identifier.scopusauthoridZhang, M=54791790500en_US
dc.identifier.scopusauthoridGao, DW=7202964911en_US
dc.identifier.scopusauthoridZhu, HG=54791807300en_US
dc.identifier.scopusauthoridHuang, QG=54791084200en_US
dc.identifier.scopusauthoridFang, HHP=7402542625en_US
dc.identifier.citeulike10186358-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats