Article: Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge
| Title | Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Zhang, T1 Zhang, XX1 Ye, L1 | ||||||
| Issue Date | 2011 | ||||||
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||
| Citation | PLoS One, 2011, v. 6 n. 10, article no. e26041 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0026041 | ||||||
| Abstract | The overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs. | ||||||
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||
| DOI | http://dx.doi.org/10.1371/journal.pone.0026041 | ||||||
| ISI Accession Number ID | WOS:000295971700034
Funding Information: This study was financially supported by the Research Grants Council of Hong Kong (HKU 7201/11E) and (HKU7202/09E), Small Project Fund (201007176069) and SPACE Research Fund of the University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||
| PubMed Central ID | PMC3189950 | ||||||
| References | References in Scopus | ||||||
| Grants | Occurrence, abundance and diversity of tetracycline resistance genes in sewage treatment plants |
| dc.contributor.author | Zhang, T | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Zhang, XX | ||||||
| dc.contributor.author | Ye, L | ||||||
| dc.date.accessioned | 2012-06-26T06:06:05Z | ||||||
| dc.date.available | 2012-06-26T06:06:05Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | The overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs. | ||||||
| dc.description.grant | Occurrence, abundance and diversity of tetracycline resistance genes in sewage treatment plants | ||||||
| dc.description.grantcode | 103927 | ||||||
| dc.description.nature | published_or_final_version | ||||||
| dc.identifier.citation | PLoS One, 2011, v. 6 n. 10, article no. e26041 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0026041 | ||||||
| dc.identifier.citeulike | 10786111 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0026041 | ||||||
| dc.identifier.hkuros | 208087 | ||||||
| dc.identifier.isi | WOS:000295971700034
Funding Information: This study was financially supported by the Research Grants Council of Hong Kong (HKU 7201/11E) and (HKU7202/09E), Small Project Fund (201007176069) and SPACE Research Fund of the University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||
| dc.identifier.issue | 10, article no. e26041 | ||||||
| dc.identifier.pmcid | PMC3189950 | ||||||
| dc.identifier.pmid | 22016806 | ||||||
| dc.identifier.scopus | eid_2-s2.0-80053908791 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/150606 | ||||||
| dc.identifier.volume | 6 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | PLoS One | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||
| dc.subject.mesh | Anti-Bacterial Agents - pharmacology | ||||||
| dc.subject.mesh | Drug Resistance, Bacterial - genetics | ||||||
| dc.subject.mesh | Interspersed Repetitive Sequences - genetics | ||||||
| dc.subject.mesh | Metagenome - drug effects - genetics | ||||||
| dc.subject.mesh | Plasmids - drug effects - genetics | ||||||
| dc.title | Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong