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Article: Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge
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TitlePlasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge
 
AuthorsZhang, T1
Zhang, XX1
Ye, L1
 
Issue Date2011
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPLoS One, 2011, v. 6 n. 10, article no. e26041 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0026041
 
AbstractThe overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs.
 
ISSN1932-6203
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.512
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0026041
 
PubMed Central IDPMC3189950
 
ISI Accession Number IDWOS:000295971700034
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU 7201/11E
HKU7202/09E
University of Hong Kong201007176069
Funding Information:

This study was financially supported by the Research Grants Council of Hong Kong (HKU 7201/11E) and (HKU7202/09E), Small Project Fund (201007176069) and SPACE Research Fund of the University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhang, T
 
dc.contributor.authorZhang, XX
 
dc.contributor.authorYe, L
 
dc.date.accessioned2012-06-26T06:06:05Z
 
dc.date.available2012-06-26T06:06:05Z
 
dc.date.issued2011
 
dc.description.abstractThe overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPLoS One, 2011, v. 6 n. 10, article no. e26041 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0026041
 
dc.identifier.citeulike10786111
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0026041
 
dc.identifier.hkuros208087
 
dc.identifier.isiWOS:000295971700034
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU 7201/11E
HKU7202/09E
University of Hong Kong201007176069
Funding Information:

This study was financially supported by the Research Grants Council of Hong Kong (HKU 7201/11E) and (HKU7202/09E), Small Project Fund (201007176069) and SPACE Research Fund of the University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
dc.identifier.issn1932-6203
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.512
 
dc.identifier.issue10, article no. e26041
 
dc.identifier.pmcidPMC3189950
 
dc.identifier.pmid22016806
 
dc.identifier.scopuseid_2-s2.0-80053908791
 
dc.identifier.urihttp://hdl.handle.net/10722/150606
 
dc.identifier.volume6
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS One
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshAnti-Bacterial Agents - pharmacology
 
dc.subject.meshDrug Resistance, Bacterial - genetics
 
dc.subject.meshInterspersed Repetitive Sequences - genetics
 
dc.subject.meshMetagenome - drug effects - genetics
 
dc.subject.meshPlasmids - drug effects - genetics
 
dc.titlePlasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong