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Article: Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge

TitlePlasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge
Authors
Issue Date2011
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2011, v. 6 n. 10, article no. e26041 How to Cite?
Abstract
The overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs.
Persistent Identifierhttp://hdl.handle.net/10722/150606
ISSN
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU 7201/11E
HKU7202/09E
University of Hong Kong201007176069
Funding Information:

This study was financially supported by the Research Grants Council of Hong Kong (HKU 7201/11E) and (HKU7202/09E), Small Project Fund (201007176069) and SPACE Research Fund of the University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorZhang, Ten_US
dc.contributor.authorZhang, XXen_US
dc.contributor.authorYe, Len_US
dc.date.accessioned2012-06-26T06:06:05Z-
dc.date.available2012-06-26T06:06:05Z-
dc.date.issued2011en_US
dc.identifier.citationPLoS One, 2011, v. 6 n. 10, article no. e26041en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10722/150606-
dc.description.abstractThe overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs.en_US
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_US
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAnti-Bacterial Agents - pharmacology-
dc.subject.meshDrug Resistance, Bacterial - genetics-
dc.subject.meshInterspersed Repetitive Sequences - genetics-
dc.subject.meshMetagenome - drug effects - genetics-
dc.subject.meshPlasmids - drug effects - genetics-
dc.titlePlasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludgeen_US
dc.typeArticleen_US
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_US
dc.identifier.emailZhang, XX: zhangxx@hku.hk-
dc.identifier.authorityZhang, T=rp00211en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1371/journal.pone.0026041en_US
dc.identifier.pmid22016806-
dc.identifier.pmcidPMC3189950-
dc.identifier.scopuseid_2-s2.0-80053908791en_US
dc.identifier.hkuros208087-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053908791&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6en_US
dc.identifier.issue10, article no. e26041en_US
dc.identifier.isiWOS:000295971700034-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYe, L=36451639300en_US
dc.identifier.scopusauthoridZhang, XX=35239161000en_US
dc.identifier.scopusauthoridZhang, T=24470677400en_US
dc.identifier.citeulike10786111-

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