Article: Ammonia-oxidizing bacteria dominates over ammonia-oxidizing archaea in a saline nitrification reactor under low DO and high nitrogen loading

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TitleAmmonia-oxidizing bacteria dominates over ammonia-oxidizing archaea in a saline nitrification reactor under low DO and high nitrogen loading
AuthorsYe, L1
Zhang, T1
Issue Date2011
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/71002188
CitationBiotechnology And Bioengineering, 2011, v. 108 n. 11, p. 2544-2552 [How to Cite?]
DOI: http://dx.doi.org/10.1002/bit.23211
AbstractA continuous nitrification reactor treating saline wastewater was operated for almost 1 year under low dissolved oxygen (DO) levels (0.15-0.5mg/L) and high nitrogen loadings (0.26-0.52kg-N/(m 3day)) in four phases. The diversity and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were analyzed by cloning, terminal restriction fragment length polymorphism (T-RFLP) and quantitative polymerase chain reaction (qPCR). The results showed that there were only one dominant AOA species and one dominant AOB species in the reactor in all of the four experimental phases. The amoA gene of the dominant AOA only had a similarity of 89.3% with the cultured AOA species Nitrosopumilus maritimus SCM1. All of the AOB species detected in the reactor belong to Nitrosomonas genus and it was found that the AOB populations changed with the ammonium loadings and DO levels. The abundance of AOB in the reactor was ∼40 times larger than that of AOA, and the ratio of AOB to AOA increased significantly up to ∼2,000 to ∼4,000 with the increase of ammonium loading, indicating that AOB are much more competitive than AOA in high ammonium environments and probably AOA play a less important role than AOB in the nitrification reactors. © 2011 Wiley Periodicals, Inc.
ISSN0006-3592
2011 Impact Factor: 3.946
2011 SCImago Journal Rankings: 0.304
DOIhttp://dx.doi.org/10.1002/bit.23211
ISI Accession Number IDWOS:000295717700004
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
Funding Information:

Contract grant sponsor: Hong Kong General Research Fund

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorYe, L
dc.contributor.authorZhang, T
dc.date.accessioned2012-06-26T06:06:03Z
dc.date.available2012-06-26T06:06:03Z
dc.date.issued2011
dc.description.abstractA continuous nitrification reactor treating saline wastewater was operated for almost 1 year under low dissolved oxygen (DO) levels (0.15-0.5mg/L) and high nitrogen loadings (0.26-0.52kg-N/(m 3day)) in four phases. The diversity and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were analyzed by cloning, terminal restriction fragment length polymorphism (T-RFLP) and quantitative polymerase chain reaction (qPCR). The results showed that there were only one dominant AOA species and one dominant AOB species in the reactor in all of the four experimental phases. The amoA gene of the dominant AOA only had a similarity of 89.3% with the cultured AOA species Nitrosopumilus maritimus SCM1. All of the AOB species detected in the reactor belong to Nitrosomonas genus and it was found that the AOB populations changed with the ammonium loadings and DO levels. The abundance of AOB in the reactor was ∼40 times larger than that of AOA, and the ratio of AOB to AOA increased significantly up to ∼2,000 to ∼4,000 with the increase of ammonium loading, indicating that AOB are much more competitive than AOA in high ammonium environments and probably AOA play a less important role than AOB in the nitrification reactors. © 2011 Wiley Periodicals, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBiotechnology And Bioengineering, 2011, v. 108 n. 11, p. 2544-2552 [How to Cite?]
DOI: http://dx.doi.org/10.1002/bit.23211
dc.identifier.doihttp://dx.doi.org/10.1002/bit.23211
dc.identifier.epage2552
dc.identifier.hkuros208077
dc.identifier.isiWOS:000295717700004
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
Funding Information:

Contract grant sponsor: Hong Kong General Research Fund

dc.identifier.issn0006-3592
2011 Impact Factor: 3.946
2011 SCImago Journal Rankings: 0.304
dc.identifier.issue11
dc.identifier.pmid21618465
dc.identifier.scopuseid_2-s2.0-80053053871
dc.identifier.spage2544
dc.identifier.urihttp://hdl.handle.net/10722/150601
dc.identifier.volume108
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/71002188
dc.publisher.placeUnited States
dc.relation.ispartofBiotechnology and Bioengineering
dc.relation.referencesReferences in Scopus
dc.subject.meshAmmonia - Metabolism
dc.subject.meshArchaea - Classification - Genetics - Metabolism
dc.subject.meshBacteria - Classification - Genetics - Metabolism
dc.subject.meshBiodiversity
dc.subject.meshBioreactors - Microbiology
dc.subject.meshCulture Media - Chemistry
dc.subject.meshDna Fingerprinting
dc.subject.meshDna, Archaeal - Genetics
dc.subject.meshDna, Bacterial - Genetics
dc.subject.meshMolecular Sequence Data
dc.subject.meshNitrification
dc.subject.meshNitrogen - Metabolism
dc.subject.meshOxidation-Reduction
dc.subject.meshOxygen - Metabolism
dc.subject.meshPolymorphism, Restriction Fragment Length
dc.subject.meshReal-Time Polymerase Chain Reaction
dc.subject.meshSalinity
dc.subject.meshSequence Analysis, Dna
dc.subject.meshWater Microbiology
dc.titleAmmonia-oxidizing bacteria dominates over ammonia-oxidizing archaea in a saline nitrification reactor under low DO and high nitrogen loading
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong