Article: Probing roles of lipopolysaccharide, type 1 fimbria, and colanic acid in the attachment of Escherichia coli strains on inert surfaces
| Title | Probing roles of lipopolysaccharide, type 1 fimbria, and colanic acid in the attachment of Escherichia coli strains on inert surfaces | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Chao, Y1 Zhang, T1 | ||||||
| Issue Date | 2011 | ||||||
| Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/langmuir | ||||||
| Citation | Langmuir, 2011, v. 27 n. 18, p. 11545-11553 [How to Cite?] DOI: http://dx.doi.org/10.1021/la202534p | ||||||
| Abstract | The roles of bacterial surface polymers in reversible (phase I) and irreversible (phase II) attachment (i.e., lipopolysaccharides (LPS), type 1 fimbria, and capsular colanic acid (CA)) were investigated in situ by combining fluorescence microscopy and atomic force microscopy. Fluorescence microscopy was used to evaluate the phase I attachment by counting the total number of cells on the substrata, and AFM was applied to image the phase II cells and measure the lateral detachment force to characterize phase II attachment. Also, by comparing the number of cells in phases I and II, the transformation ratio was calculated and used as an index to evaluate the roles of different polymers in the attachment process. Escherichia coli K-12 and its six mutants, which had different surface polymers in terms of LPS structures, CA contents, and type 1 fimbriae, were used as the test strains. Six different materials were applied as substrata, including glass, two metals (aluminum and stainless steel), and three plastics (polyvinyl chloride, polycarbonate, and polyethylene). The results indicated that LPS significantly enhanced phases I and II attachment as well as the transformation ratio from phase I to II. Like LPS, type 1 fimbriae largely increased the phase I attachment and the transformation ratio; however, they did not significantly influence the adhesion strength in phase II. CA had a negative effect on attachment in phases I and II by decreasing the adhered number of cells and the lateral detachment force, respectively, but had no influence on the transformation ratio. © 2011 American Chemical Society. | ||||||
| ISSN | 0743-7463 2011 Impact Factor: 4.186 2011 SCImago Journal Rankings: 0.381 | ||||||
| DOI | http://dx.doi.org/10.1021/la202534p | ||||||
| ISI Accession Number ID | WOS:000294790500035
Funding Information: We thank the Hong Kong UGC One-Off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study. Y.C. thanks the HKU for a postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated. | ||||||
| References | References in Scopus |
| dc.contributor.author | Chao, Y | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Zhang, T | ||||||
| dc.date.accessioned | 2012-06-26T06:06:02Z | ||||||
| dc.date.available | 2012-06-26T06:06:02Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | The roles of bacterial surface polymers in reversible (phase I) and irreversible (phase II) attachment (i.e., lipopolysaccharides (LPS), type 1 fimbria, and capsular colanic acid (CA)) were investigated in situ by combining fluorescence microscopy and atomic force microscopy. Fluorescence microscopy was used to evaluate the phase I attachment by counting the total number of cells on the substrata, and AFM was applied to image the phase II cells and measure the lateral detachment force to characterize phase II attachment. Also, by comparing the number of cells in phases I and II, the transformation ratio was calculated and used as an index to evaluate the roles of different polymers in the attachment process. Escherichia coli K-12 and its six mutants, which had different surface polymers in terms of LPS structures, CA contents, and type 1 fimbriae, were used as the test strains. Six different materials were applied as substrata, including glass, two metals (aluminum and stainless steel), and three plastics (polyvinyl chloride, polycarbonate, and polyethylene). The results indicated that LPS significantly enhanced phases I and II attachment as well as the transformation ratio from phase I to II. Like LPS, type 1 fimbriae largely increased the phase I attachment and the transformation ratio; however, they did not significantly influence the adhesion strength in phase II. CA had a negative effect on attachment in phases I and II by decreasing the adhered number of cells and the lateral detachment force, respectively, but had no influence on the transformation ratio. © 2011 American Chemical Society. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Langmuir, 2011, v. 27 n. 18, p. 11545-11553 [How to Cite?] DOI: http://dx.doi.org/10.1021/la202534p | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1021/la202534p | ||||||
| dc.identifier.epage | 11553 | ||||||
| dc.identifier.hkuros | 208088 | ||||||
| dc.identifier.isi | WOS:000294790500035
Funding Information: We thank the Hong Kong UGC One-Off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study. Y.C. thanks the HKU for a postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated. | ||||||
| dc.identifier.issn | 0743-7463 2011 Impact Factor: 4.186 2011 SCImago Journal Rankings: 0.381 | ||||||
| dc.identifier.issue | 18 | ||||||
| dc.identifier.pmid | 21842859 | ||||||
| dc.identifier.scopus | eid_2-s2.0-80052718478 | ||||||
| dc.identifier.spage | 11545 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/150599 | ||||||
| dc.identifier.volume | 27 | ||||||
| dc.language | eng | ||||||
| dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/langmuir | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Langmuir | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Bacterial Adhesion | ||||||
| dc.subject.mesh | Cell Count | ||||||
| dc.subject.mesh | Escherichia Coli K12 - Cytology - Metabolism | ||||||
| dc.subject.mesh | Fimbriae Proteins - Metabolism | ||||||
| dc.subject.mesh | Lipopolysaccharides - Metabolism | ||||||
| dc.subject.mesh | Microbial Viability | ||||||
| dc.subject.mesh | Microscopy, Atomic Force | ||||||
| dc.subject.mesh | Microscopy, Fluorescence | ||||||
| dc.subject.mesh | Physicochemical Processes | ||||||
| dc.subject.mesh | Polysaccharides - Metabolism | ||||||
| dc.subject.mesh | Surface Properties | ||||||
| dc.title | Probing roles of lipopolysaccharide, type 1 fimbria, and colanic acid in the attachment of Escherichia coli strains on inert surfaces | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong