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Article: Probing roles of lipopolysaccharide, type 1 fimbria, and colanic acid in the attachment of Escherichia coli strains on inert surfaces
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TitleProbing roles of lipopolysaccharide, type 1 fimbria, and colanic acid in the attachment of Escherichia coli strains on inert surfaces
 
AuthorsChao, Y1
Zhang, T1
 
Issue Date2011
 
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/langmuir
 
CitationLangmuir, 2011, v. 27 n. 18, p. 11545-11553 [How to Cite?]
DOI: http://dx.doi.org/10.1021/la202534p
 
AbstractThe roles of bacterial surface polymers in reversible (phase I) and irreversible (phase II) attachment (i.e., lipopolysaccharides (LPS), type 1 fimbria, and capsular colanic acid (CA)) were investigated in situ by combining fluorescence microscopy and atomic force microscopy. Fluorescence microscopy was used to evaluate the phase I attachment by counting the total number of cells on the substrata, and AFM was applied to image the phase II cells and measure the lateral detachment force to characterize phase II attachment. Also, by comparing the number of cells in phases I and II, the transformation ratio was calculated and used as an index to evaluate the roles of different polymers in the attachment process. Escherichia coli K-12 and its six mutants, which had different surface polymers in terms of LPS structures, CA contents, and type 1 fimbriae, were used as the test strains. Six different materials were applied as substrata, including glass, two metals (aluminum and stainless steel), and three plastics (polyvinyl chloride, polycarbonate, and polyethylene). The results indicated that LPS significantly enhanced phases I and II attachment as well as the transformation ratio from phase I to II. Like LPS, type 1 fimbriae largely increased the phase I attachment and the transformation ratio; however, they did not significantly influence the adhesion strength in phase II. CA had a negative effect on attachment in phases I and II by decreasing the adhered number of cells and the lateral detachment force, respectively, but had no influence on the transformation ratio. © 2011 American Chemical Society.
 
ISSN0743-7463
2013 Impact Factor: 4.384
 
DOIhttp://dx.doi.org/10.1021/la202534p
 
ISI Accession Number IDWOS:000294790500035
Funding AgencyGrant Number
Hong Kong UGCSEG HKU10
HKU
Funding Information:

We thank the Hong Kong UGC One-Off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study. Y.C. thanks the HKU for a postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChao, Y
 
dc.contributor.authorZhang, T
 
dc.date.accessioned2012-06-26T06:06:02Z
 
dc.date.available2012-06-26T06:06:02Z
 
dc.date.issued2011
 
dc.description.abstractThe roles of bacterial surface polymers in reversible (phase I) and irreversible (phase II) attachment (i.e., lipopolysaccharides (LPS), type 1 fimbria, and capsular colanic acid (CA)) were investigated in situ by combining fluorescence microscopy and atomic force microscopy. Fluorescence microscopy was used to evaluate the phase I attachment by counting the total number of cells on the substrata, and AFM was applied to image the phase II cells and measure the lateral detachment force to characterize phase II attachment. Also, by comparing the number of cells in phases I and II, the transformation ratio was calculated and used as an index to evaluate the roles of different polymers in the attachment process. Escherichia coli K-12 and its six mutants, which had different surface polymers in terms of LPS structures, CA contents, and type 1 fimbriae, were used as the test strains. Six different materials were applied as substrata, including glass, two metals (aluminum and stainless steel), and three plastics (polyvinyl chloride, polycarbonate, and polyethylene). The results indicated that LPS significantly enhanced phases I and II attachment as well as the transformation ratio from phase I to II. Like LPS, type 1 fimbriae largely increased the phase I attachment and the transformation ratio; however, they did not significantly influence the adhesion strength in phase II. CA had a negative effect on attachment in phases I and II by decreasing the adhered number of cells and the lateral detachment force, respectively, but had no influence on the transformation ratio. © 2011 American Chemical Society.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationLangmuir, 2011, v. 27 n. 18, p. 11545-11553 [How to Cite?]
DOI: http://dx.doi.org/10.1021/la202534p
 
dc.identifier.doihttp://dx.doi.org/10.1021/la202534p
 
dc.identifier.eissn1520-5827
 
dc.identifier.epage11553
 
dc.identifier.hkuros208088
 
dc.identifier.isiWOS:000294790500035
Funding AgencyGrant Number
Hong Kong UGCSEG HKU10
HKU
Funding Information:

We thank the Hong Kong UGC One-Off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study. Y.C. thanks the HKU for a postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated.

 
dc.identifier.issn0743-7463
2013 Impact Factor: 4.384
 
dc.identifier.issue18
 
dc.identifier.pmid21842859
 
dc.identifier.scopuseid_2-s2.0-80052718478
 
dc.identifier.spage11545
 
dc.identifier.urihttp://hdl.handle.net/10722/150599
 
dc.identifier.volume27
 
dc.languageeng
 
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/langmuir
 
dc.publisher.placeUnited States
 
dc.relation.ispartofLangmuir
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBacterial Adhesion
 
dc.subject.meshCell Count
 
dc.subject.meshEscherichia Coli K12 - Cytology - Metabolism
 
dc.subject.meshFimbriae Proteins - Metabolism
 
dc.subject.meshLipopolysaccharides - Metabolism
 
dc.subject.meshMicrobial Viability
 
dc.subject.meshMicroscopy, Atomic Force
 
dc.subject.meshMicroscopy, Fluorescence
 
dc.subject.meshPhysicochemical Processes
 
dc.subject.meshPolysaccharides - Metabolism
 
dc.subject.meshSurface Properties
 
dc.titleProbing roles of lipopolysaccharide, type 1 fimbria, and colanic acid in the attachment of Escherichia coli strains on inert surfaces
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong