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Article: Pathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing
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TitlePathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing
 
AuthorsYe, L1
Zhang, T1
 
Issue Date2011
 
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
 
CitationEnvironmental Science And Technology, 2011, v. 45 n. 17, p. 7173-7179 [How to Cite?]
DOI: http://dx.doi.org/10.1021/es201045e
 
AbstractThis study applied 454 high-throughput pyrosequencing to analyze potentially pathogenic bacteria in activated sludge from 14 municipal wastewater treatment plants (WWTPs) across four countries (China, U.S., Canada, and Singapore), plus the influent and effluent of one of the 14 WWTPs. A total of 370-870 16S rRNA gene sequences with average length of 207 bps were obtained and all of them were assigned to corresponding taxonomic ranks by using RDP classifier and MEGAN. It was found that the most abundant potentially pathogenic bacteria in the WWTPs were affiliated with the genera of Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila, and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Some sequences highly similar (>99%) to Corynebacterium diphtheriae were found in the influent and activated sludge samples from a saline WWTP. Overall, the percentage of the sequences closely related (>99%) to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a platform-independent Java application (BAND) was developed for graphical visualization of the data of microbial abundance generated by high-throughput pyrosequencing. The approach demonstrated in this study could examine most of the potentially pathogenic bacteria simultaneously instead of one-by-one detection by other methods. © 2011 American Chemical Society.
 
ISSN0013-936X
2013 Impact Factor: 5.481
 
DOIhttp://dx.doi.org/10.1021/es201045e
 
ISI Accession Number IDWOS:000294373400014
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
University of Hong Kong
Funding Information:

We thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study and Lin Ye thanks The University of Hong Kong for the postgraduate studentship. We would like to thank Prof. Gao D.W., Prof. Deng B. L., Dr. Huang Q G., Dr. Zhu H. G., Dr. Liang D. W., Dr. Duan J. Z., Dr. Zhang M., Dr. Zhang X. X. for the activated sludge sampling.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYe, L
 
dc.contributor.authorZhang, T
 
dc.date.accessioned2012-06-26T06:05:59Z
 
dc.date.available2012-06-26T06:05:59Z
 
dc.date.issued2011
 
dc.description.abstractThis study applied 454 high-throughput pyrosequencing to analyze potentially pathogenic bacteria in activated sludge from 14 municipal wastewater treatment plants (WWTPs) across four countries (China, U.S., Canada, and Singapore), plus the influent and effluent of one of the 14 WWTPs. A total of 370-870 16S rRNA gene sequences with average length of 207 bps were obtained and all of them were assigned to corresponding taxonomic ranks by using RDP classifier and MEGAN. It was found that the most abundant potentially pathogenic bacteria in the WWTPs were affiliated with the genera of Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila, and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Some sequences highly similar (>99%) to Corynebacterium diphtheriae were found in the influent and activated sludge samples from a saline WWTP. Overall, the percentage of the sequences closely related (>99%) to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a platform-independent Java application (BAND) was developed for graphical visualization of the data of microbial abundance generated by high-throughput pyrosequencing. The approach demonstrated in this study could examine most of the potentially pathogenic bacteria simultaneously instead of one-by-one detection by other methods. © 2011 American Chemical Society.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationEnvironmental Science And Technology, 2011, v. 45 n. 17, p. 7173-7179 [How to Cite?]
DOI: http://dx.doi.org/10.1021/es201045e
 
dc.identifier.doihttp://dx.doi.org/10.1021/es201045e
 
dc.identifier.eissn1520-5851
 
dc.identifier.epage7179
 
dc.identifier.hkuros208076
 
dc.identifier.isiWOS:000294373400014
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
University of Hong Kong
Funding Information:

We thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study and Lin Ye thanks The University of Hong Kong for the postgraduate studentship. We would like to thank Prof. Gao D.W., Prof. Deng B. L., Dr. Huang Q G., Dr. Zhu H. G., Dr. Liang D. W., Dr. Duan J. Z., Dr. Zhang M., Dr. Zhang X. X. for the activated sludge sampling.

 
dc.identifier.issn0013-936X
2013 Impact Factor: 5.481
 
dc.identifier.issue17
 
dc.identifier.pmid21780772
 
dc.identifier.scopuseid_2-s2.0-80052240448
 
dc.identifier.spage7173
 
dc.identifier.urihttp://hdl.handle.net/10722/150594
 
dc.identifier.volume45
 
dc.languageeng
 
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
 
dc.publisher.placeUnited States
 
dc.relation.ispartofEnvironmental Science and Technology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBacteria - Classification - Genetics - Pathogenicity
 
dc.subject.meshBase Sequence
 
dc.subject.meshCanada
 
dc.subject.meshChina
 
dc.subject.meshHigh-Throughput Nucleotide Sequencing - Methods
 
dc.subject.meshHumans
 
dc.subject.meshPhylogeny
 
dc.subject.meshPolymerase Chain Reaction - Methods
 
dc.subject.meshRna, Ribosomal, 16S - Genetics
 
dc.subject.meshSewage - Microbiology
 
dc.subject.meshSingapore
 
dc.subject.meshUnited States
 
dc.titlePathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong