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Article: Pathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing

TitlePathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing
Authors
Issue Date2011
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
Citation
Environmental Science And Technology, 2011, v. 45 n. 17, p. 7173-7179 How to Cite?
Abstract
This study applied 454 high-throughput pyrosequencing to analyze potentially pathogenic bacteria in activated sludge from 14 municipal wastewater treatment plants (WWTPs) across four countries (China, U.S., Canada, and Singapore), plus the influent and effluent of one of the 14 WWTPs. A total of 370-870 16S rRNA gene sequences with average length of 207 bps were obtained and all of them were assigned to corresponding taxonomic ranks by using RDP classifier and MEGAN. It was found that the most abundant potentially pathogenic bacteria in the WWTPs were affiliated with the genera of Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila, and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Some sequences highly similar (>99%) to Corynebacterium diphtheriae were found in the influent and activated sludge samples from a saline WWTP. Overall, the percentage of the sequences closely related (>99%) to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a platform-independent Java application (BAND) was developed for graphical visualization of the data of microbial abundance generated by high-throughput pyrosequencing. The approach demonstrated in this study could examine most of the potentially pathogenic bacteria simultaneously instead of one-by-one detection by other methods. © 2011 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/150594
ISSN
2013 Impact Factor: 5.481
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
University of Hong Kong
Funding Information:

We thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study and Lin Ye thanks The University of Hong Kong for the postgraduate studentship. We would like to thank Prof. Gao D.W., Prof. Deng B. L., Dr. Huang Q G., Dr. Zhu H. G., Dr. Liang D. W., Dr. Duan J. Z., Dr. Zhang M., Dr. Zhang X. X. for the activated sludge sampling.

References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorYe, Len_US
dc.contributor.authorZhang, Ten_US
dc.date.accessioned2012-06-26T06:05:59Z-
dc.date.available2012-06-26T06:05:59Z-
dc.date.issued2011en_US
dc.identifier.citationEnvironmental Science And Technology, 2011, v. 45 n. 17, p. 7173-7179en_US
dc.identifier.issn0013-936Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/150594-
dc.description.abstractThis study applied 454 high-throughput pyrosequencing to analyze potentially pathogenic bacteria in activated sludge from 14 municipal wastewater treatment plants (WWTPs) across four countries (China, U.S., Canada, and Singapore), plus the influent and effluent of one of the 14 WWTPs. A total of 370-870 16S rRNA gene sequences with average length of 207 bps were obtained and all of them were assigned to corresponding taxonomic ranks by using RDP classifier and MEGAN. It was found that the most abundant potentially pathogenic bacteria in the WWTPs were affiliated with the genera of Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila, and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Some sequences highly similar (>99%) to Corynebacterium diphtheriae were found in the influent and activated sludge samples from a saline WWTP. Overall, the percentage of the sequences closely related (>99%) to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a platform-independent Java application (BAND) was developed for graphical visualization of the data of microbial abundance generated by high-throughput pyrosequencing. The approach demonstrated in this study could examine most of the potentially pathogenic bacteria simultaneously instead of one-by-one detection by other methods. © 2011 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/esten_US
dc.relation.ispartofEnvironmental Science and Technologyen_US
dc.subject.meshBacteria - Classification - Genetics - Pathogenicityen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCanadaen_US
dc.subject.meshChinaen_US
dc.subject.meshHigh-Throughput Nucleotide Sequencing - Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshRna, Ribosomal, 16S - Geneticsen_US
dc.subject.meshSewage - Microbiologyen_US
dc.subject.meshSingaporeen_US
dc.subject.meshUnited Statesen_US
dc.titlePathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencingen_US
dc.typeArticleen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_US
dc.identifier.authorityZhang, T=rp00211en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/es201045een_US
dc.identifier.pmid21780772en_US
dc.identifier.scopuseid_2-s2.0-80052240448en_US
dc.identifier.hkuros208076-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052240448&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue17en_US
dc.identifier.spage7173en_US
dc.identifier.epage7179en_US
dc.identifier.eissn1520-5851-
dc.identifier.isiWOS:000294373400014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYe, L=36451639300en_US
dc.identifier.scopusauthoridZhang, T=24470677400en_US

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