Article: Pathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing
| Title | Pathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Ye, L1 Zhang, T1 | ||||||
| Issue Date | 2011 | ||||||
| Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/est | ||||||
| Citation | Environmental Science And Technology, 2011, v. 45 n. 17, p. 7173-7179 [How to Cite?] DOI: http://dx.doi.org/10.1021/es201045e | ||||||
| Abstract | This study applied 454 high-throughput pyrosequencing to analyze potentially pathogenic bacteria in activated sludge from 14 municipal wastewater treatment plants (WWTPs) across four countries (China, U.S., Canada, and Singapore), plus the influent and effluent of one of the 14 WWTPs. A total of 370-870 16S rRNA gene sequences with average length of 207 bps were obtained and all of them were assigned to corresponding taxonomic ranks by using RDP classifier and MEGAN. It was found that the most abundant potentially pathogenic bacteria in the WWTPs were affiliated with the genera of Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila, and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Some sequences highly similar (>99%) to Corynebacterium diphtheriae were found in the influent and activated sludge samples from a saline WWTP. Overall, the percentage of the sequences closely related (>99%) to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a platform-independent Java application (BAND) was developed for graphical visualization of the data of microbial abundance generated by high-throughput pyrosequencing. The approach demonstrated in this study could examine most of the potentially pathogenic bacteria simultaneously instead of one-by-one detection by other methods. © 2011 American Chemical Society. | ||||||
| ISSN | 0013-936X 2011 Impact Factor: 5.228 2011 SCImago Journal Rankings: 0.305 | ||||||
| DOI | http://dx.doi.org/10.1021/es201045e | ||||||
| ISI Accession Number ID | WOS:000294373400014
Funding Information: We thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study and Lin Ye thanks The University of Hong Kong for the postgraduate studentship. We would like to thank Prof. Gao D.W., Prof. Deng B. L., Dr. Huang Q G., Dr. Zhu H. G., Dr. Liang D. W., Dr. Duan J. Z., Dr. Zhang M., Dr. Zhang X. X. for the activated sludge sampling. | ||||||
| References | References in Scopus |
| dc.contributor.author | Ye, L | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Zhang, T | ||||||
| dc.date.accessioned | 2012-06-26T06:05:59Z | ||||||
| dc.date.available | 2012-06-26T06:05:59Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | This study applied 454 high-throughput pyrosequencing to analyze potentially pathogenic bacteria in activated sludge from 14 municipal wastewater treatment plants (WWTPs) across four countries (China, U.S., Canada, and Singapore), plus the influent and effluent of one of the 14 WWTPs. A total of 370-870 16S rRNA gene sequences with average length of 207 bps were obtained and all of them were assigned to corresponding taxonomic ranks by using RDP classifier and MEGAN. It was found that the most abundant potentially pathogenic bacteria in the WWTPs were affiliated with the genera of Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila, and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Some sequences highly similar (>99%) to Corynebacterium diphtheriae were found in the influent and activated sludge samples from a saline WWTP. Overall, the percentage of the sequences closely related (>99%) to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a platform-independent Java application (BAND) was developed for graphical visualization of the data of microbial abundance generated by high-throughput pyrosequencing. The approach demonstrated in this study could examine most of the potentially pathogenic bacteria simultaneously instead of one-by-one detection by other methods. © 2011 American Chemical Society. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Environmental Science And Technology, 2011, v. 45 n. 17, p. 7173-7179 [How to Cite?] DOI: http://dx.doi.org/10.1021/es201045e | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1021/es201045e | ||||||
| dc.identifier.epage | 7179 | ||||||
| dc.identifier.hkuros | 208076 | ||||||
| dc.identifier.isi | WOS:000294373400014
Funding Information: We thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study and Lin Ye thanks The University of Hong Kong for the postgraduate studentship. We would like to thank Prof. Gao D.W., Prof. Deng B. L., Dr. Huang Q G., Dr. Zhu H. G., Dr. Liang D. W., Dr. Duan J. Z., Dr. Zhang M., Dr. Zhang X. X. for the activated sludge sampling. | ||||||
| dc.identifier.issn | 0013-936X 2011 Impact Factor: 5.228 2011 SCImago Journal Rankings: 0.305 | ||||||
| dc.identifier.issue | 17 | ||||||
| dc.identifier.pmid | 21780772 | ||||||
| dc.identifier.scopus | eid_2-s2.0-80052240448 | ||||||
| dc.identifier.spage | 7173 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/150594 | ||||||
| dc.identifier.volume | 45 | ||||||
| dc.language | eng | ||||||
| dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/est | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Environmental Science and Technology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Bacteria - Classification - Genetics - Pathogenicity | ||||||
| dc.subject.mesh | Base Sequence | ||||||
| dc.subject.mesh | Canada | ||||||
| dc.subject.mesh | China | ||||||
| dc.subject.mesh | High-Throughput Nucleotide Sequencing - Methods | ||||||
| dc.subject.mesh | Humans | ||||||
| dc.subject.mesh | Phylogeny | ||||||
| dc.subject.mesh | Polymerase Chain Reaction - Methods | ||||||
| dc.subject.mesh | Rna, Ribosomal, 16S - Genetics | ||||||
| dc.subject.mesh | Sewage - Microbiology | ||||||
| dc.subject.mesh | Singapore | ||||||
| dc.subject.mesh | United States | ||||||
| dc.title | Pathogenic bacteria in sewage treatment plants as revealed by 454 pyrosequencing | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong