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Article: Application of molecular techniques on heterotrophic hydrogen production research

TitleApplication of molecular techniques on heterotrophic hydrogen production research
Authors
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortech
Citation
Bioresource Technology, 2011, v. 102 n. 18, p. 8445-8456 How to Cite?
AbstractThis paper reviews the application of molecular techniques in heterotrophic hydrogen production studies. Commonly used molecular techniques are introduced briefly first, including cloning-sequencing after polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), terminal-restriction fragment length polymorphism (T-RFLP), fluorescence in situ hybridization (FISH) and quantitative real-time PCR. Application of the molecular techniques in heterotrophic hydrogen production studies are discussed in details, focusing on identification of new isolates for hydrogen production, characterization of microbial compositions in bioreactors, monitoring microbial diversity variation, visualization of microbial distribution in hydrogen-producing granular sludge, and quantification of various microbial populations. Some significant findings in recent hydrogen production studies with the application of molecular techniques are discussed, followed by a research outlook of the heterotrophic biohydrogen field. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/150586
ISSN
2015 Impact Factor: 4.917
2015 SCImago Journal Rankings: 2.303
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong General Research Fund7125/09E
MU ICEE
Funding Information:

The authors wish to thank the Hong Kong General Research Fund (7125/09E) and MU ICEE funding for the financial support of this study.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, RYen_US
dc.contributor.authorZhang, Ten_US
dc.contributor.authorFang, HHPen_US
dc.date.accessioned2012-06-26T06:05:56Z-
dc.date.available2012-06-26T06:05:56Z-
dc.date.issued2011en_US
dc.identifier.citationBioresource Technology, 2011, v. 102 n. 18, p. 8445-8456en_US
dc.identifier.issn0960-8524en_US
dc.identifier.urihttp://hdl.handle.net/10722/150586-
dc.description.abstractThis paper reviews the application of molecular techniques in heterotrophic hydrogen production studies. Commonly used molecular techniques are introduced briefly first, including cloning-sequencing after polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), terminal-restriction fragment length polymorphism (T-RFLP), fluorescence in situ hybridization (FISH) and quantitative real-time PCR. Application of the molecular techniques in heterotrophic hydrogen production studies are discussed in details, focusing on identification of new isolates for hydrogen production, characterization of microbial compositions in bioreactors, monitoring microbial diversity variation, visualization of microbial distribution in hydrogen-producing granular sludge, and quantification of various microbial populations. Some significant findings in recent hydrogen production studies with the application of molecular techniques are discussed, followed by a research outlook of the heterotrophic biohydrogen field. © 2011 Elsevier Ltd.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortechen_US
dc.relation.ispartofBioresource Technologyen_US
dc.subject.meshBiotechnology - Methodsen_US
dc.subject.meshHeterotrophic Processes - Physiologyen_US
dc.subject.meshHydrogen - Metabolismen_US
dc.subject.meshMolecular Biology - Methodsen_US
dc.subject.meshResearch - Methodsen_US
dc.titleApplication of molecular techniques on heterotrophic hydrogen production researchen_US
dc.typeArticleen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_US
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_US
dc.identifier.authorityZhang, T=rp00211en_US
dc.identifier.authorityFang, HHP=rp00115en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.biortech.2011.02.072en_US
dc.identifier.pmid21398117-
dc.identifier.scopuseid_2-s2.0-80051676702en_US
dc.identifier.hkuros208089-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80051676702&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume102en_US
dc.identifier.issue18en_US
dc.identifier.spage8445en_US
dc.identifier.epage8456en_US
dc.identifier.eissn1873-2976-
dc.identifier.isiWOS:000295107200014-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLi, RY=47361444200en_US
dc.identifier.scopusauthoridZhang, T=24470677400en_US
dc.identifier.scopusauthoridFang, HHP=7402542625en_US
dc.identifier.citeulike8892516-

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