File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Analysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing

TitleAnalysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing
Authors
Keywords454 high-throughput pyrosequencing
Activated sludge
Bacterial community
Cloning
16s rrna gene
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watres
Citation
Water Research, 2011, v. 45 n. 15, p. 4390-4398 How to Cite?
AbstractFor full understanding of the microbial community in the wastewater treatment bioreactors, one of the feasible and effective ways is to investigate the massive genetic information contained in the activated sludge. In this study, high-throughput pyrosequencing was applied to analyze the 16S rRNA gene of bacteria in a laboratory-scale nitrification reactor and a full-scale wastewater treatment plant. In total, 27,458 and 26,906 effective sequence reads of the 16S rRNA gene were obtained from the Reactor and the wastewater treatment plant activated sludge samples respectively. The taxonomic complexities in the two samples were compared at phylum and genus levels. According to the pyrosequencing results, even for a laboratory-scale reactor as simple as that in this study, a small size clone library is far from enough to reflect the whole profile of the bacterial community. In addition, it was found that the commonly used informatics tool ' RDP classifier' may drastically assign Nitrosomonas sequences into a wrong taxonomic unit resulting in underestimation of ammonia-oxidizing bacteria in the bioreactors. In this paper the reasons for this mistakenly assignment were analyzed and correction methods were proposed. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/150579
ISSN
2014 Impact Factor: 5.528
2013 SCImago Journal Rankings: 3.026
ISI Accession Number ID
Funding AgencyGrant Number
HKU
Hong Kong General Research FundHKU7197-08E
Funding Information:

Dr. Ming-Fei Shao thanks HKU for the postdoctoral fellowship. Lin Ye thanks HKU for the postgraduate studentship. We also would like to thank Hong Kong General Research Fund (HKU7197-08E) for financial support of this study and W Chan and CK Wong for technical help in pyrosequencing.

References

 

DC FieldValueLanguage
dc.contributor.authorYe, Len_HK
dc.contributor.authorShao, Men_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorTong, AHYen_HK
dc.contributor.authorLok, Sen_HK
dc.date.accessioned2012-06-26T06:05:54Z-
dc.date.available2012-06-26T06:05:54Z-
dc.date.issued2011en_HK
dc.identifier.citationWater Research, 2011, v. 45 n. 15, p. 4390-4398en_HK
dc.identifier.issn0043-1354en_HK
dc.identifier.urihttp://hdl.handle.net/10722/150579-
dc.description.abstractFor full understanding of the microbial community in the wastewater treatment bioreactors, one of the feasible and effective ways is to investigate the massive genetic information contained in the activated sludge. In this study, high-throughput pyrosequencing was applied to analyze the 16S rRNA gene of bacteria in a laboratory-scale nitrification reactor and a full-scale wastewater treatment plant. In total, 27,458 and 26,906 effective sequence reads of the 16S rRNA gene were obtained from the Reactor and the wastewater treatment plant activated sludge samples respectively. The taxonomic complexities in the two samples were compared at phylum and genus levels. According to the pyrosequencing results, even for a laboratory-scale reactor as simple as that in this study, a small size clone library is far from enough to reflect the whole profile of the bacterial community. In addition, it was found that the commonly used informatics tool ' RDP classifier' may drastically assign Nitrosomonas sequences into a wrong taxonomic unit resulting in underestimation of ammonia-oxidizing bacteria in the bioreactors. In this paper the reasons for this mistakenly assignment were analyzed and correction methods were proposed. © 2011 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watresen_HK
dc.relation.ispartofWater Researchen_HK
dc.subject454 high-throughput pyrosequencingen_HK
dc.subjectActivated sludgeen_HK
dc.subjectBacterial communityen_HK
dc.subjectCloningen_HK
dc.subject16s rrna gene-
dc.subject.meshWater Purification - Methodsen_US
dc.subject.meshWaste Disposal, Fluid - Methodsen_US
dc.subject.meshSewage - Microbiologyen_US
dc.subject.meshSequence Analysis, Dna - Methodsen_US
dc.subject.meshRna, Ribosomal, 16S - Analysisen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshNitrosomonas - Geneticsen_US
dc.subject.meshNitrificationen_US
dc.subject.meshIndustrial Waste - Prevention & Controlen_US
dc.subject.meshDna, Bacterial - Analysis - Classification - Geneticsen_US
dc.subject.meshBioreactors - Microbiologyen_US
dc.subject.meshBiodegradation, Environmentalen_US
dc.subject.meshBacteria - Classification - Geneticsen_US
dc.subject.meshAmmonia - Analysisen_US
dc.titleAnalysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencingen_HK
dc.typeArticleen_HK
dc.identifier.emailYe, L: linye@hku.hken_HK
dc.identifier.emailShao, M: mingfei_shao@yahoo.com.cnen_HK
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.emailTong, AHY: amytong@hkucc.hku.hk-
dc.identifier.emailLok, S: silok@genome.hku.hk-
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.watres.2011.05.028en_HK
dc.identifier.pmid21705039en_HK
dc.identifier.scopuseid_2-s2.0-79960558447en_HK
dc.identifier.hkuros208083-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960558447&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue15en_HK
dc.identifier.spage4390en_HK
dc.identifier.epage4398en_HK
dc.identifier.isiWOS:000294101300007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.scopusauthoridTong, AHY=7103351716en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridShao, MF=34868583400en_HK
dc.identifier.scopusauthoridYe, L=36451639300en_HK
dc.identifier.citeulike9415709-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats