Article: Characterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes
| Title | Characterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Authors | Li, J1 2 Zhang, T1 Wang, L2 Liu, Y2 Dai, R2 Liu, X2 | ||||||||||||
| Issue Date | 2011 | ||||||||||||
| Publisher | Taylor & Francis. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09593330.asp | ||||||||||||
| Citation | Environmental Technology, 2011, v. 32 n. 5, p. 533-542 [How to Cite?] DOI: http://dx.doi.org/10.1080/09593330.2010.504749 | ||||||||||||
| Abstract | The effect of nickel on the microbial community in the activated sludge of a sequencing batch reactor (SBR) reactor was investigated by continuously dosing nickel from 60 to 240 mg Ni(II) L -1. The diversity of the microbial community was investigated by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis of the variable V3 region of the bacterial 16S rDNA. The experimental results showed that the community structure changed significantly after dosing with nickel with a shift in dominant species, the disappearance of some original species and the emergence of some new species. The existence of a nickel resistant gene was also investigated using PCR. The obtained nickel resistance gene had a maximum homology with the plasmid pMOL30 of Ralstonia metallidurans CH34. The quantitative real-time PCR results indicated that the quantity of the nickel resistance gene was related to the nickel concentration loaded to the reactor. © 2011 Taylor &Francis. | ||||||||||||
| ISSN | 0959-3330 2011 Impact Factor: 1.406 2011 SCImago Journal Rankings: 0.065 | ||||||||||||
| DOI | http://dx.doi.org/10.1080/09593330.2010.504749 | ||||||||||||
| ISI Accession Number ID | WOS:000290406200007
Funding Information: Jia Li especially wishes to thank the Hong Kong General Research Fund (7195/06E) for research assistantship, and all the authors thank the Chinese Natural Science Foundation (No. 20377010), China High-tech Research and Development (863) Plan (No. 2008AA062501), the Research Foundation of the Ministry of Environmental Protection of the People's Republic of China (No. 200809024) and The Foundation of the State Key Laboratory of Pollution Control and Resources Reuse (No. PCRRF09003) for financial support for this study. | ||||||||||||
| References | References in Scopus |
| dc.contributor.author | Li, J | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Zhang, T | ||||||||||||
| dc.contributor.author | Wang, L | ||||||||||||
| dc.contributor.author | Liu, Y | ||||||||||||
| dc.contributor.author | Dai, R | ||||||||||||
| dc.contributor.author | Liu, X | ||||||||||||
| dc.date.accessioned | 2012-06-26T06:05:45Z | ||||||||||||
| dc.date.available | 2012-06-26T06:05:45Z | ||||||||||||
| dc.date.issued | 2011 | ||||||||||||
| dc.description.abstract | The effect of nickel on the microbial community in the activated sludge of a sequencing batch reactor (SBR) reactor was investigated by continuously dosing nickel from 60 to 240 mg Ni(II) L -1. The diversity of the microbial community was investigated by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis of the variable V3 region of the bacterial 16S rDNA. The experimental results showed that the community structure changed significantly after dosing with nickel with a shift in dominant species, the disappearance of some original species and the emergence of some new species. The existence of a nickel resistant gene was also investigated using PCR. The obtained nickel resistance gene had a maximum homology with the plasmid pMOL30 of Ralstonia metallidurans CH34. The quantitative real-time PCR results indicated that the quantity of the nickel resistance gene was related to the nickel concentration loaded to the reactor. © 2011 Taylor &Francis. | ||||||||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||||||||
| dc.identifier.citation | Environmental Technology, 2011, v. 32 n. 5, p. 533-542 [How to Cite?] DOI: http://dx.doi.org/10.1080/09593330.2010.504749 | ||||||||||||
| dc.identifier.doi | http://dx.doi.org/10.1080/09593330.2010.504749 | ||||||||||||
| dc.identifier.epage | 542 | ||||||||||||
| dc.identifier.hkuros | 192709 | ||||||||||||
| dc.identifier.hkuros | 208085 | ||||||||||||
| dc.identifier.isi | WOS:000290406200007
Funding Information: Jia Li especially wishes to thank the Hong Kong General Research Fund (7195/06E) for research assistantship, and all the authors thank the Chinese Natural Science Foundation (No. 20377010), China High-tech Research and Development (863) Plan (No. 2008AA062501), the Research Foundation of the Ministry of Environmental Protection of the People's Republic of China (No. 200809024) and The Foundation of the State Key Laboratory of Pollution Control and Resources Reuse (No. PCRRF09003) for financial support for this study. | ||||||||||||
| dc.identifier.issn | 0959-3330 2011 Impact Factor: 1.406 2011 SCImago Journal Rankings: 0.065 | ||||||||||||
| dc.identifier.issue | 5 | ||||||||||||
| dc.identifier.pmid | 21877534 | ||||||||||||
| dc.identifier.scopus | eid_2-s2.0-79956350560 | ||||||||||||
| dc.identifier.spage | 533 | ||||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/150567 | ||||||||||||
| dc.identifier.volume | 32 | ||||||||||||
| dc.language | eng | ||||||||||||
| dc.publisher | Taylor & Francis. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09593330.asp | ||||||||||||
| dc.publisher.place | United Kingdom | ||||||||||||
| dc.relation.ispartof | Environmental Technology | ||||||||||||
| dc.relation.references | References in Scopus | ||||||||||||
| dc.subject.mesh | Cluster Analysis | ||||||||||||
| dc.subject.mesh | Dna, Bacterial - Chemistry - Genetics | ||||||||||||
| dc.subject.mesh | Dna, Ribosomal - Chemistry - Genetics | ||||||||||||
| dc.subject.mesh | Denaturing Gradient Gel Electrophoresis | ||||||||||||
| dc.subject.mesh | Nickel - Pharmacology | ||||||||||||
| dc.subject.mesh | Ralstonia - Drug Effects - Genetics - Metabolism | ||||||||||||
| dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | ||||||||||||
| dc.subject.mesh | Sequence Analysis, Dna | ||||||||||||
| dc.subject.mesh | Sewage - Microbiology | ||||||||||||
| dc.subject.mesh | Water Pollutants, Chemical - Pharmacology | ||||||||||||
| dc.title | Characterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes | ||||||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Fudan University