File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes

TitleCharacterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes
Authors
Issue Date2011
PublisherTaylor & Francis. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09593330.asp
Citation
Environmental Technology, 2011, v. 32 n. 5, p. 533-542 How to Cite?
Abstract
The effect of nickel on the microbial community in the activated sludge of a sequencing batch reactor (SBR) reactor was investigated by continuously dosing nickel from 60 to 240 mg Ni(II) L -1. The diversity of the microbial community was investigated by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis of the variable V3 region of the bacterial 16S rDNA. The experimental results showed that the community structure changed significantly after dosing with nickel with a shift in dominant species, the disappearance of some original species and the emergence of some new species. The existence of a nickel resistant gene was also investigated using PCR. The obtained nickel resistance gene had a maximum homology with the plasmid pMOL30 of Ralstonia metallidurans CH34. The quantitative real-time PCR results indicated that the quantity of the nickel resistance gene was related to the nickel concentration loaded to the reactor. © 2011 Taylor &Francis.
Persistent Identifierhttp://hdl.handle.net/10722/150567
ISSN
2013 Impact Factor: 1.197
2013 SCImago Journal Rankings: 0.494
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong General Research Fund7195/06E
Chinese Natural Science Foundation20377010
China High-tech Research and Development (863) Plan2008AA062501
Research Foundation of the Ministry of Environmental Protection of the People's Republic of China200809024
Foundation of the State Key Laboratory of Pollution Control and Resources ReusePCRRF09003
Funding Information:

Jia Li especially wishes to thank the Hong Kong General Research Fund (7195/06E) for research assistantship, and all the authors thank the Chinese Natural Science Foundation (No. 20377010), China High-tech Research and Development (863) Plan (No. 2008AA062501), the Research Foundation of the Ministry of Environmental Protection of the People's Republic of China (No. 200809024) and The Foundation of the State Key Laboratory of Pollution Control and Resources Reuse (No. PCRRF09003) for financial support for this study.

References

 

Author Affiliations
  1. The University of Hong Kong
  2. Fudan University
DC FieldValueLanguage
dc.contributor.authorLi, Jen_US
dc.contributor.authorZhang, Ten_US
dc.contributor.authorWang, Len_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorDai, Ren_US
dc.contributor.authorLiu, Xen_US
dc.date.accessioned2012-06-26T06:05:45Z-
dc.date.available2012-06-26T06:05:45Z-
dc.date.issued2011en_US
dc.identifier.citationEnvironmental Technology, 2011, v. 32 n. 5, p. 533-542en_US
dc.identifier.issn0959-3330en_US
dc.identifier.urihttp://hdl.handle.net/10722/150567-
dc.description.abstractThe effect of nickel on the microbial community in the activated sludge of a sequencing batch reactor (SBR) reactor was investigated by continuously dosing nickel from 60 to 240 mg Ni(II) L -1. The diversity of the microbial community was investigated by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis of the variable V3 region of the bacterial 16S rDNA. The experimental results showed that the community structure changed significantly after dosing with nickel with a shift in dominant species, the disappearance of some original species and the emergence of some new species. The existence of a nickel resistant gene was also investigated using PCR. The obtained nickel resistance gene had a maximum homology with the plasmid pMOL30 of Ralstonia metallidurans CH34. The quantitative real-time PCR results indicated that the quantity of the nickel resistance gene was related to the nickel concentration loaded to the reactor. © 2011 Taylor &Francis.en_US
dc.languageengen_US
dc.publisherTaylor & Francis. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09593330.aspen_US
dc.relation.ispartofEnvironmental Technologyen_US
dc.subject.meshCluster Analysisen_US
dc.subject.meshDna, Bacterial - Chemistry - Geneticsen_US
dc.subject.meshDna, Ribosomal - Chemistry - Geneticsen_US
dc.subject.meshDenaturing Gradient Gel Electrophoresisen_US
dc.subject.meshNickel - Pharmacologyen_US
dc.subject.meshRalstonia - Drug Effects - Genetics - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSewage - Microbiologyen_US
dc.subject.meshWater Pollutants, Chemical - Pharmacologyen_US
dc.titleCharacterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genesen_US
dc.typeArticleen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_US
dc.identifier.authorityZhang, T=rp00211en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/09593330.2010.504749en_US
dc.identifier.pmid21877534en_US
dc.identifier.scopuseid_2-s2.0-79956350560en_US
dc.identifier.hkuros192709-
dc.identifier.hkuros208085-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956350560&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume32en_US
dc.identifier.issue5en_US
dc.identifier.spage533en_US
dc.identifier.epage542en_US
dc.identifier.isiWOS:000290406200007-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLi, J=35078835800en_US
dc.identifier.scopusauthoridZhang, T=24470677400en_US
dc.identifier.scopusauthoridWang, L=36241024800en_US
dc.identifier.scopusauthoridLiu, Y=36013132500en_US
dc.identifier.scopusauthoridDai, R=36240421000en_US
dc.identifier.scopusauthoridLiu, X=36240659000en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats