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Article: Characterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes
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TitleCharacterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes
 
AuthorsLi, J1 2
Zhang, T1
Wang, L2
Liu, Y2
Dai, R2
Liu, X2
 
Issue Date2011
 
PublisherTaylor & Francis. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09593330.asp
 
CitationEnvironmental Technology, 2011, v. 32 n. 5, p. 533-542 [How to Cite?]
DOI: http://dx.doi.org/10.1080/09593330.2010.504749
 
AbstractThe effect of nickel on the microbial community in the activated sludge of a sequencing batch reactor (SBR) reactor was investigated by continuously dosing nickel from 60 to 240 mg Ni(II) L -1. The diversity of the microbial community was investigated by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis of the variable V3 region of the bacterial 16S rDNA. The experimental results showed that the community structure changed significantly after dosing with nickel with a shift in dominant species, the disappearance of some original species and the emergence of some new species. The existence of a nickel resistant gene was also investigated using PCR. The obtained nickel resistance gene had a maximum homology with the plasmid pMOL30 of Ralstonia metallidurans CH34. The quantitative real-time PCR results indicated that the quantity of the nickel resistance gene was related to the nickel concentration loaded to the reactor. © 2011 Taylor &Francis.
 
ISSN0959-3330
2012 Impact Factor: 1.606
2012 SCImago Journal Rankings: 0.565
 
DOIhttp://dx.doi.org/10.1080/09593330.2010.504749
 
ISI Accession Number IDWOS:000290406200007
Funding AgencyGrant Number
Hong Kong General Research Fund7195/06E
Chinese Natural Science Foundation20377010
China High-tech Research and Development (863) Plan2008AA062501
Research Foundation of the Ministry of Environmental Protection of the People's Republic of China200809024
Foundation of the State Key Laboratory of Pollution Control and Resources ReusePCRRF09003
Funding Information:

Jia Li especially wishes to thank the Hong Kong General Research Fund (7195/06E) for research assistantship, and all the authors thank the Chinese Natural Science Foundation (No. 20377010), China High-tech Research and Development (863) Plan (No. 2008AA062501), the Research Foundation of the Ministry of Environmental Protection of the People's Republic of China (No. 200809024) and The Foundation of the State Key Laboratory of Pollution Control and Resources Reuse (No. PCRRF09003) for financial support for this study.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, J
 
dc.contributor.authorZhang, T
 
dc.contributor.authorWang, L
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorDai, R
 
dc.contributor.authorLiu, X
 
dc.date.accessioned2012-06-26T06:05:45Z
 
dc.date.available2012-06-26T06:05:45Z
 
dc.date.issued2011
 
dc.description.abstractThe effect of nickel on the microbial community in the activated sludge of a sequencing batch reactor (SBR) reactor was investigated by continuously dosing nickel from 60 to 240 mg Ni(II) L -1. The diversity of the microbial community was investigated by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis of the variable V3 region of the bacterial 16S rDNA. The experimental results showed that the community structure changed significantly after dosing with nickel with a shift in dominant species, the disappearance of some original species and the emergence of some new species. The existence of a nickel resistant gene was also investigated using PCR. The obtained nickel resistance gene had a maximum homology with the plasmid pMOL30 of Ralstonia metallidurans CH34. The quantitative real-time PCR results indicated that the quantity of the nickel resistance gene was related to the nickel concentration loaded to the reactor. © 2011 Taylor &Francis.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationEnvironmental Technology, 2011, v. 32 n. 5, p. 533-542 [How to Cite?]
DOI: http://dx.doi.org/10.1080/09593330.2010.504749
 
dc.identifier.doihttp://dx.doi.org/10.1080/09593330.2010.504749
 
dc.identifier.epage542
 
dc.identifier.hkuros192709
 
dc.identifier.hkuros208085
 
dc.identifier.isiWOS:000290406200007
Funding AgencyGrant Number
Hong Kong General Research Fund7195/06E
Chinese Natural Science Foundation20377010
China High-tech Research and Development (863) Plan2008AA062501
Research Foundation of the Ministry of Environmental Protection of the People's Republic of China200809024
Foundation of the State Key Laboratory of Pollution Control and Resources ReusePCRRF09003
Funding Information:

Jia Li especially wishes to thank the Hong Kong General Research Fund (7195/06E) for research assistantship, and all the authors thank the Chinese Natural Science Foundation (No. 20377010), China High-tech Research and Development (863) Plan (No. 2008AA062501), the Research Foundation of the Ministry of Environmental Protection of the People's Republic of China (No. 200809024) and The Foundation of the State Key Laboratory of Pollution Control and Resources Reuse (No. PCRRF09003) for financial support for this study.

 
dc.identifier.issn0959-3330
2012 Impact Factor: 1.606
2012 SCImago Journal Rankings: 0.565
 
dc.identifier.issue5
 
dc.identifier.pmid21877534
 
dc.identifier.scopuseid_2-s2.0-79956350560
 
dc.identifier.spage533
 
dc.identifier.urihttp://hdl.handle.net/10722/150567
 
dc.identifier.volume32
 
dc.languageeng
 
dc.publisherTaylor & Francis. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09593330.asp
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofEnvironmental Technology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCluster Analysis
 
dc.subject.meshDna, Bacterial - Chemistry - Genetics
 
dc.subject.meshDna, Ribosomal - Chemistry - Genetics
 
dc.subject.meshDenaturing Gradient Gel Electrophoresis
 
dc.subject.meshNickel - Pharmacology
 
dc.subject.meshRalstonia - Drug Effects - Genetics - Metabolism
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshSequence Analysis, Dna
 
dc.subject.meshSewage - Microbiology
 
dc.subject.meshWater Pollutants, Chemical - Pharmacology
 
dc.titleCharacterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Fudan University