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Article: Occurrence of ammonia-oxidizing Archaea in activated sludges of a laboratory scale reactor and two wastewater treatment plants
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TitleOccurrence of ammonia-oxidizing Archaea in activated sludges of a laboratory scale reactor and two wastewater treatment plants
 
AuthorsZhang, T2
Jin, T2
Yan, Q2
Shao, M2
Wells, G1
Criddle, C1
Fang, HHP2
 
Issue Date2009
 
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM
 
CitationJournal of Applied Microbiology, 2009, v. 107 n. 3, p. 970-977 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1365-2672.2009.04283.x
 
AbstractAims: Characterization of the ammonia-oxidizing archaea (AOA) community in activated sludge from a nitrogen removal bioreactor and wastewater treatment plants (WWTPs). Methods and Results: Three primer sets specific for ammonia mono-oxygenase α-subunit (amoA) were used to construct clone libraries for activated sludge sample from a nitrogen removal bioreactor. One primer set resulted in strong nonspecific PCR products. The other two clone libraries retrieved both shared and unique AOA amoA sequences. One primer set was chosen to study the AOA communities of activated sludge samples from Shatin and Stanley WWTPs. In total, 18 AOA amoA sequences were recovered and compared to the previous reported sequences. A phylogenetic analysis indicated that sequences found in this study fell into three clusters. Conclusions: Different primers resulted in varied AOA communities from the same sample. The AOA found from Hong Kong WWTPs were closely similar to those from sediment and soil, but distinct from those from activated sludge in other places. A comparison of clone libraries between Shatin WWTP and bioreactor indicated the AOA community significantly shifted only after 30-day enrichment. Significance and Impact of the Study: This study confirmed the occurrence of AOA in a laboratory scale nitrogen removal bioreactor and Hong Kong WWTPs treating saline or freshwater wastewater. AOA communities found in this study were significantly different from those found in other places. To retrieve diverse AOA communities from environmental samples, a combination of different primers for the amoA gene is needed. © 2009 The Society for Applied Microbiology.
 
ISSN1364-5072
2012 Impact Factor: 2.196
2012 SCImago Journal Rankings: 0.824
 
DOIhttp://dx.doi.org/10.1111/j.1365-2672.2009.04283.x
 
ISI Accession Number IDWOS:000268854000028
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
Funding Information:

The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study, and Qingmei Yan and Meifei Shao wish to thank HKU for the postgraduate studentship.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhang, T
 
dc.contributor.authorJin, T
 
dc.contributor.authorYan, Q
 
dc.contributor.authorShao, M
 
dc.contributor.authorWells, G
 
dc.contributor.authorCriddle, C
 
dc.contributor.authorFang, HHP
 
dc.date.accessioned2012-06-26T06:05:16Z
 
dc.date.available2012-06-26T06:05:16Z
 
dc.date.issued2009
 
dc.description.abstractAims: Characterization of the ammonia-oxidizing archaea (AOA) community in activated sludge from a nitrogen removal bioreactor and wastewater treatment plants (WWTPs). Methods and Results: Three primer sets specific for ammonia mono-oxygenase α-subunit (amoA) were used to construct clone libraries for activated sludge sample from a nitrogen removal bioreactor. One primer set resulted in strong nonspecific PCR products. The other two clone libraries retrieved both shared and unique AOA amoA sequences. One primer set was chosen to study the AOA communities of activated sludge samples from Shatin and Stanley WWTPs. In total, 18 AOA amoA sequences were recovered and compared to the previous reported sequences. A phylogenetic analysis indicated that sequences found in this study fell into three clusters. Conclusions: Different primers resulted in varied AOA communities from the same sample. The AOA found from Hong Kong WWTPs were closely similar to those from sediment and soil, but distinct from those from activated sludge in other places. A comparison of clone libraries between Shatin WWTP and bioreactor indicated the AOA community significantly shifted only after 30-day enrichment. Significance and Impact of the Study: This study confirmed the occurrence of AOA in a laboratory scale nitrogen removal bioreactor and Hong Kong WWTPs treating saline or freshwater wastewater. AOA communities found in this study were significantly different from those found in other places. To retrieve diverse AOA communities from environmental samples, a combination of different primers for the amoA gene is needed. © 2009 The Society for Applied Microbiology.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal of Applied Microbiology, 2009, v. 107 n. 3, p. 970-977 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1365-2672.2009.04283.x
 
dc.identifier.citeulike5487556
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1365-2672.2009.04283.x
 
dc.identifier.epage977
 
dc.identifier.hkuros175516
 
dc.identifier.isiWOS:000268854000028
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
Funding Information:

The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study, and Qingmei Yan and Meifei Shao wish to thank HKU for the postgraduate studentship.

 
dc.identifier.issn1364-5072
2012 Impact Factor: 2.196
2012 SCImago Journal Rankings: 0.824
 
dc.identifier.issue3
 
dc.identifier.pmid19486399
 
dc.identifier.scopuseid_2-s2.0-68849114735
 
dc.identifier.spage970
 
dc.identifier.urihttp://hdl.handle.net/10722/150504
 
dc.identifier.volume107
 
dc.languageeng
 
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Applied Microbiology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAmmonia - Metabolism
 
dc.subject.meshArchaea - Enzymology - Genetics - Isolation & Purification
 
dc.subject.meshArchaeal Proteins - Genetics
 
dc.subject.meshBioreactors - Microbiology
 
dc.subject.meshDna Primers
 
dc.subject.meshDna, Archaeal - Analysis
 
dc.subject.meshGenetic Variation
 
dc.subject.meshHong Kong
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshOxidoreductases - Genetics
 
dc.subject.meshSequence Analysis, Dna
 
dc.subject.meshSewage - Microbiology
 
dc.subject.meshWaste Disposal, Fluid
 
dc.subject.meshWater Microbiology
 
dc.titleOccurrence of ammonia-oxidizing Archaea in activated sludges of a laboratory scale reactor and two wastewater treatment plants
 
dc.typeArticle
 
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Author Affiliations
  1. Stanford University
  2. The University of Hong Kong