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Article: Direct detection of cell surface interactive forces of sessile, fimbriated and non-fimbriated Actinomyces spp. using atomic force microscopy

TitleDirect detection of cell surface interactive forces of sessile, fimbriated and non-fimbriated Actinomyces spp. using atomic force microscopy
Authors
KeywordsActinomyces
Adhesion and aggregation
Atomic force microscopy
Fimbriae
Interaction force
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2004, v. 49 n. 9, p. 727-738 How to Cite?
AbstractActinomyces species are predominant early colonizers of the oral cavity and prime mediators of inter-bacterial adhesion and coaggregation. Previous workers have evaluated the adhesion of Actinomyces spp. by quantitative assessment of sessile, as opposed to planktonic cells attached to substrates, but did not quantify the cell surface interactive forces. Therefore we used atomic force microscopy to directly detect the interactive force between an approaching silicon tip and sessile Actinomyces spp. adhering to a substrate, at nanonewton (nN) range force levels. A total of eight strains each belonging to fimbriated and non-fimbriated Actinomyces species were employed, namely A. bovis, A. gerencseriae, A. israelii, A. meyeri, A. naeslundii genospecies 1 and 2, A. odontolyticus and A. viscosus. The sterile mica discs, used as the adhesion substrate, were immersed in mono-species bacterial suspensions for five days to obtain a thin bacterial biofilm. Interactive forces were measured using a silicon nitride cantilever attached to a Nanoscope IIIA atomic force microscope. The interactive forces between the approaching silicon nitride tip and bacterial biofilm surfaces were randomly quantified at three different locations on each cell; namely, the cell surface proper, the periphery of the cell and the substrate and, the interface between two cells. When the interactive forces at these locations of the same species were compared, significantly higher force levels at the cell-cell interface than the other two locations were noted with A. gerencseriae (P < 0.001), A. viscosus (P < 0.01) and A. israelii (P < 0.05). When the interactive forces of different Actinomyces spp. at an identical location were compared, fimbriated A. naeslundii genospecies 2 showed the greatest interactive force at the cell surface proper (-32.6 ± 8.7 nN, P < 0.01). A. naeslundii genospecies 1, 2 and A. viscosus demonstrated greater interactive force at the cell-mica periphery than the other five species (P < 0.05); A. viscosus (-34.6 ± 10.5 nN) displayed greater interactive force at the cell-cell interface than the others (P < 0.01), except for A. gerencseriae (P > 0.05). These data indicate that fimbriated Actinomyces spp., including A. naeslundii genospecies 1, 2 and A. viscosus exert higher cell surface interactive forces than those devoid of fimbriae and, such variable force levels may modulate their adhesion and coaggregation during biofilm formation. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/150350
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, Gen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorChan, KYen_HK
dc.contributor.authorLuo, Gen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2012-06-26T06:03:45Z-
dc.date.available2012-06-26T06:03:45Z-
dc.date.issued2004en_HK
dc.identifier.citationArchives Of Oral Biology, 2004, v. 49 n. 9, p. 727-738en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/150350-
dc.description.abstractActinomyces species are predominant early colonizers of the oral cavity and prime mediators of inter-bacterial adhesion and coaggregation. Previous workers have evaluated the adhesion of Actinomyces spp. by quantitative assessment of sessile, as opposed to planktonic cells attached to substrates, but did not quantify the cell surface interactive forces. Therefore we used atomic force microscopy to directly detect the interactive force between an approaching silicon tip and sessile Actinomyces spp. adhering to a substrate, at nanonewton (nN) range force levels. A total of eight strains each belonging to fimbriated and non-fimbriated Actinomyces species were employed, namely A. bovis, A. gerencseriae, A. israelii, A. meyeri, A. naeslundii genospecies 1 and 2, A. odontolyticus and A. viscosus. The sterile mica discs, used as the adhesion substrate, were immersed in mono-species bacterial suspensions for five days to obtain a thin bacterial biofilm. Interactive forces were measured using a silicon nitride cantilever attached to a Nanoscope IIIA atomic force microscope. The interactive forces between the approaching silicon nitride tip and bacterial biofilm surfaces were randomly quantified at three different locations on each cell; namely, the cell surface proper, the periphery of the cell and the substrate and, the interface between two cells. When the interactive forces at these locations of the same species were compared, significantly higher force levels at the cell-cell interface than the other two locations were noted with A. gerencseriae (P < 0.001), A. viscosus (P < 0.01) and A. israelii (P < 0.05). When the interactive forces of different Actinomyces spp. at an identical location were compared, fimbriated A. naeslundii genospecies 2 showed the greatest interactive force at the cell surface proper (-32.6 ± 8.7 nN, P < 0.01). A. naeslundii genospecies 1, 2 and A. viscosus demonstrated greater interactive force at the cell-mica periphery than the other five species (P < 0.05); A. viscosus (-34.6 ± 10.5 nN) displayed greater interactive force at the cell-cell interface than the others (P < 0.01), except for A. gerencseriae (P > 0.05). These data indicate that fimbriated Actinomyces spp., including A. naeslundii genospecies 1, 2 and A. viscosus exert higher cell surface interactive forces than those devoid of fimbriae and, such variable force levels may modulate their adhesion and coaggregation during biofilm formation. © 2004 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subjectActinomycesen_HK
dc.subjectAdhesion and aggregationen_HK
dc.subjectAtomic force microscopyen_HK
dc.subjectFimbriaeen_HK
dc.subjectInteraction forceen_HK
dc.subject.meshActinomyces - Isolation & Purification - Physiology - Ultrastructureen_US
dc.subject.meshBacterial Adhesionen_US
dc.subject.meshBiophysical Phenomenaen_US
dc.subject.meshBiophysicsen_US
dc.subject.meshCell Membrane - Microbiologyen_US
dc.subject.meshGenotypeen_US
dc.subject.meshMicroscopy, Atomic Forceen_US
dc.titleDirect detection of cell surface interactive forces of sessile, fimbriated and non-fimbriated Actinomyces spp. using atomic force microscopyen_HK
dc.typeArticleen_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailChan, KY: hrsccky@hku.hken_HK
dc.identifier.emailFang, HHP: hrechef@hkucc.hku.hken_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityChan, KY=rp00662en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.archoralbio.2004.04.003en_HK
dc.identifier.pmid15275860-
dc.identifier.scopuseid_2-s2.0-3343020218en_HK
dc.identifier.hkuros89290-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3343020218&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue9en_HK
dc.identifier.spage727en_HK
dc.identifier.epage738en_HK
dc.identifier.isiWOS:000223288800006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTang, G=36882453100en_HK
dc.identifier.scopusauthoridYip, HK=25423244900en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridChan, KY=7406034142en_HK
dc.identifier.scopusauthoridLuo, G=55112399700en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK

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