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Article: Quantification of Saccharomyces cerevisiae viability using BacLight.

TitleQuantification of Saccharomyces cerevisiae viability using BacLight.
Authors
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492
Citation
Biotechnology Letters, 2004, v. 26 n. 12, p. 989-992 How to Cite?
AbstractYeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.
Persistent Identifierhttp://hdl.handle.net/10722/150287
ISSN
2015 Impact Factor: 1.639
2015 SCImago Journal Rankings: 0.591
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_US
dc.contributor.authorFang, HHen_US
dc.date.accessioned2012-06-26T06:03:02Z-
dc.date.available2012-06-26T06:03:02Z-
dc.date.issued2004en_US
dc.identifier.citationBiotechnology Letters, 2004, v. 26 n. 12, p. 989-992en_US
dc.identifier.issn0141-5492en_US
dc.identifier.urihttp://hdl.handle.net/10722/150287-
dc.description.abstractYeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492en_US
dc.relation.ispartofBiotechnology lettersen_US
dc.subject.meshCell Culture Techniques - Instrumentation - Methodsen_US
dc.subject.meshCell Survival - Physiologyen_US
dc.subject.meshColony Count, Microbial - Instrumentation - Methodsen_US
dc.subject.meshFlow Cytometry - Instrumentation - Methodsen_US
dc.subject.meshFluorescent Dyesen_US
dc.subject.meshGreen Fluorescent Proteinsen_US
dc.subject.meshMicroscopy, Confocal - Instrumentation - Methodsen_US
dc.subject.meshMicroscopy, Fluorescence - Instrumentation - Methodsen_US
dc.subject.meshPropidiumen_US
dc.subject.meshReagent Kits, Diagnosticen_US
dc.subject.meshSaccharomyces Cerevisiae - Cytology - Isolation & Purification - Physiologyen_US
dc.titleQuantification of Saccharomyces cerevisiae viability using BacLight.en_US
dc.typeArticleen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_US
dc.identifier.emailFang, HH:hrechef@hkucc.hku.hken_US
dc.identifier.authorityZhang, T=rp00211en_US
dc.identifier.authorityFang, HH=rp00115en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid15269525-
dc.identifier.scopuseid_2-s2.0-16644393440en_US
dc.identifier.hkuros93239-
dc.identifier.volume26en_US
dc.identifier.issue12en_US
dc.identifier.spage989en_US
dc.identifier.epage992en_US
dc.identifier.isiWOS:000221806100008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhang, T=24470677400en_US
dc.identifier.scopusauthoridFang, HH=7402542625en_US

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