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Article: Methanogen population in a marine biofilm corrosive to mild steel

TitleMethanogen population in a marine biofilm corrosive to mild steel
Authors
Issue Date2003
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2003, v. 63 n. 1, p. 101-106 How to Cite?
AbstractThis study was conducted to analyze the methanogen population in a corrosive marine biofilm based on 16S rDNA analysis, using a PCR-cloning-sequencing approach. There were 80 methanogen clones developed from the PCR-amplified DNA extracted from the biofilm on the mild steel surface. All clones were categorized into one of five operational taxonomy units (OTUs). Two OTUs (comprising 57 clones) were affiliated with the acetotrophic Methanosaeta genus; the remaining three OTUs (23 clones) were affiliated with the hydrogenotrophic genera of Methanogenium, Methanoplanus and Methanocalculus. The hydrogenotrophic methanogens could directly cause metal corrosion through cathodic depolarization, whereas the acetotrophic methanogens grew syntrophically with corrosion-causing sulfate-reducing bacteria, as observed by fluorescent in situ hybridization, and thus contribute indirectly to metal corrosion.
Persistent Identifierhttp://hdl.handle.net/10722/150284
ISSN
2015 Impact Factor: 3.376
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_US
dc.contributor.authorFang, HHPen_US
dc.contributor.authorKo, BCBen_US
dc.date.accessioned2012-06-26T06:03:01Z-
dc.date.available2012-06-26T06:03:01Z-
dc.date.issued2003en_US
dc.identifier.citationApplied Microbiology And Biotechnology, 2003, v. 63 n. 1, p. 101-106en_US
dc.identifier.issn0175-7598en_US
dc.identifier.urihttp://hdl.handle.net/10722/150284-
dc.description.abstractThis study was conducted to analyze the methanogen population in a corrosive marine biofilm based on 16S rDNA analysis, using a PCR-cloning-sequencing approach. There were 80 methanogen clones developed from the PCR-amplified DNA extracted from the biofilm on the mild steel surface. All clones were categorized into one of five operational taxonomy units (OTUs). Two OTUs (comprising 57 clones) were affiliated with the acetotrophic Methanosaeta genus; the remaining three OTUs (23 clones) were affiliated with the hydrogenotrophic genera of Methanogenium, Methanoplanus and Methanocalculus. The hydrogenotrophic methanogens could directly cause metal corrosion through cathodic depolarization, whereas the acetotrophic methanogens grew syntrophically with corrosion-causing sulfate-reducing bacteria, as observed by fluorescent in situ hybridization, and thus contribute indirectly to metal corrosion.en_US
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_US
dc.relation.ispartofApplied Microbiology and Biotechnologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBiofilmsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna, Archaeal - Chemistry - Geneticsen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMethanomicrobiales - Genetics - Isolation & Purificationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Ribosomal, 16S - Chemistry - Geneticsen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSulfur-Reducing Bacteriaen_US
dc.titleMethanogen population in a marine biofilm corrosive to mild steelen_US
dc.typeArticleen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_US
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_US
dc.identifier.authorityZhang, T=rp00211en_US
dc.identifier.authorityFang, HHP=rp00115en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00253-003-1396-2en_US
dc.identifier.pmid12879307-
dc.identifier.scopuseid_2-s2.0-1642440124en_US
dc.identifier.hkuros93245-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1642440124&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume63en_US
dc.identifier.issue1en_US
dc.identifier.spage101en_US
dc.identifier.epage106en_US
dc.identifier.isiWOS:000186198900015-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridZhang, T=24470677400en_US
dc.identifier.scopusauthoridFang, HHP=7402542625en_US
dc.identifier.scopusauthoridKo, BCB=36857665100en_US

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