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Article: Extraction of extracellular polymeric substances (EPS) of sludges

TitleExtraction of extracellular polymeric substances (EPS) of sludges
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotec
Citation
Journal of Biotechnology, 2002, v. 95 n. 3, p. 249-256 How to Cite?
AbstractThe efficacies of extracting extracellular polymeric substances (EPS) from aerobic, acidogenic and methanogenic sludges using EDTA, cation exchange resin and formaldehyde under various conditions were compared. Results show that formaldehye plus NaOH was most effective in extracting EPS for all sludges; only 1.1-1.2% of DNA in the sludge samples were detected, suggesting the EPS extracted were not contaminated by intracellular substances. For each gram of volatile solids, formaldehyde-NaOH extracted 165, 179 and 102 mg of EPS from aerobic, acidogenic and methanogenic sludges, respectively. All EPS were mainly composed of carbohydrate, protein and humic substance, plus small quantities of uronic acid and DNA. Carbohydrate was predominant in the acidogenic sludge (62% in the EPS extracted by formaldehyde-NaOH), whereas protein was predominant in the methanogenic sludge (41%). Humic substance, which has often been overlooked, accounted for 30.6, 8.4 and 22.8% of the extracted EPS from aerobic, acidogenic and methanogenic sludges, respectively. However, judging from EPS quantities estimated from confocal laser scanning microscopic observations, formaldehyde-NaOH extracted only a limited portion of EPS. Optimization of extraction procedures and/or development of a more effective extraction method are warranted. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/150225
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.064
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Hen_US
dc.contributor.authorFang, HHPen_US
dc.date.accessioned2012-06-26T06:02:33Z-
dc.date.available2012-06-26T06:02:33Z-
dc.date.issued2002en_US
dc.identifier.citationJournal of Biotechnology, 2002, v. 95 n. 3, p. 249-256en_US
dc.identifier.issn0168-1656en_US
dc.identifier.urihttp://hdl.handle.net/10722/150225-
dc.description.abstractThe efficacies of extracting extracellular polymeric substances (EPS) from aerobic, acidogenic and methanogenic sludges using EDTA, cation exchange resin and formaldehyde under various conditions were compared. Results show that formaldehye plus NaOH was most effective in extracting EPS for all sludges; only 1.1-1.2% of DNA in the sludge samples were detected, suggesting the EPS extracted were not contaminated by intracellular substances. For each gram of volatile solids, formaldehyde-NaOH extracted 165, 179 and 102 mg of EPS from aerobic, acidogenic and methanogenic sludges, respectively. All EPS were mainly composed of carbohydrate, protein and humic substance, plus small quantities of uronic acid and DNA. Carbohydrate was predominant in the acidogenic sludge (62% in the EPS extracted by formaldehyde-NaOH), whereas protein was predominant in the methanogenic sludge (41%). Humic substance, which has often been overlooked, accounted for 30.6, 8.4 and 22.8% of the extracted EPS from aerobic, acidogenic and methanogenic sludges, respectively. However, judging from EPS quantities estimated from confocal laser scanning microscopic observations, formaldehyde-NaOH extracted only a limited portion of EPS. Optimization of extraction procedures and/or development of a more effective extraction method are warranted. © 2002 Elsevier Science B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotecen_US
dc.relation.ispartofJournal of Biotechnologyen_US
dc.rightsJournal of Biotechnology. Copyright © Elsevier BV.-
dc.subject.meshBiopolymers - Isolation & Purificationen_US
dc.subject.meshChemistry Techniques, Analytical - Methodsen_US
dc.subject.meshSewage - Chemistryen_US
dc.titleExtraction of extracellular polymeric substances (EPS) of sludgesen_US
dc.typeArticleen_US
dc.identifier.emailFang, HHP: hrechef@hkucc.hku.hken_US
dc.identifier.authorityFang, HHP=rp00115en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0168-1656(02)00025-1en_US
dc.identifier.pmid12007865-
dc.identifier.scopuseid_2-s2.0-0037161635en_US
dc.identifier.hkuros66949-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037161635&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume95en_US
dc.identifier.issue3en_US
dc.identifier.spage249en_US
dc.identifier.epage256en_US
dc.identifier.isiWOS:000176322400006-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLiu, H=36072584800en_US
dc.identifier.scopusauthoridFang, HHP=7402542625en_US

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