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Article: Histological analysis of microstructure of UASB granules

TitleHistological analysis of microstructure of UASB granules
Authors
Issue Date1994
PublisherAmerican Society of Civil Engineers. The Journal's web site is located at http://www.pubs.asce.org/journals/ee.html
Citation
Journal Of Environmental Engineering, 1994, v. 120 n. 5, p. 1322-1326 How to Cite?
AbstractA histological method was demonstrated for the microscopic analysis of granules from upflow-anaerobic-sludge-blanket (UASB) reactors. Granules were first fixed with formaldehyde and embedded in paraffin, before being sectioned by a microtome to a thickness of 3-5 μm. The section was then mounted on a glass slide for microscopic examination using phase contrast and epi-fluorescent excitation. Using this simple method, the microstructure of the granules and the distribution of methanogenic bacteria could be analyzed. Over 100 granules from UASB reactors treating sucrose wastewaters were examined. Microscopic photos showed that the granules had a surface layer comprised of hydrogen-consuming methanogenic cocci and bacilli, and a loosely packed interior, mainly comprised of acetotrophic Methanothrix. This simple method provides engineers with a practical tool for studying the microstructure of the granules, the mechanisms of their formation, and kinetic modeling. | A histological method was demonstrated for the microscopic analysis of granules from upflow-anaerobic-sludge-blanket (UASB) reactors. Granules were first fixed with formaldehyde and embedded in paraffin, before being sectioned by a microtome to a thickness of 3-5 μm. The section was then mounted on a glass slide for microscopic examination using phase contrast and epi-fluorescent excitation. Using this simple method, the microstructure of the granules and the distribution of methanogenic bacteria could be analyzed. Over 100 granules from UASB reactors treating sucrose wastewaters were examined. Microscopic photos showed that the granules had a surface layer comprised of hydrogen-consuming methanogenic cocci and bacilli, and a loosely packed interior, mainly comprised of acetotrophic Methanothrix. This simple method provides engineers with a practical tool for studying the microstructure of the granules, the mechanisms of their formation, and kinetic modeling.
Persistent Identifierhttp://hdl.handle.net/10722/150032
ISSN
2015 Impact Factor: 1.125
2015 SCImago Journal Rankings: 0.462

 

DC FieldValueLanguage
dc.contributor.authorChui, HKen_US
dc.contributor.authorFang, HHPen_US
dc.date.accessioned2012-06-26T06:01:12Z-
dc.date.available2012-06-26T06:01:12Z-
dc.date.issued1994en_US
dc.identifier.citationJournal Of Environmental Engineering, 1994, v. 120 n. 5, p. 1322-1326en_US
dc.identifier.issn0733-9372en_US
dc.identifier.urihttp://hdl.handle.net/10722/150032-
dc.description.abstractA histological method was demonstrated for the microscopic analysis of granules from upflow-anaerobic-sludge-blanket (UASB) reactors. Granules were first fixed with formaldehyde and embedded in paraffin, before being sectioned by a microtome to a thickness of 3-5 μm. The section was then mounted on a glass slide for microscopic examination using phase contrast and epi-fluorescent excitation. Using this simple method, the microstructure of the granules and the distribution of methanogenic bacteria could be analyzed. Over 100 granules from UASB reactors treating sucrose wastewaters were examined. Microscopic photos showed that the granules had a surface layer comprised of hydrogen-consuming methanogenic cocci and bacilli, and a loosely packed interior, mainly comprised of acetotrophic Methanothrix. This simple method provides engineers with a practical tool for studying the microstructure of the granules, the mechanisms of their formation, and kinetic modeling. | A histological method was demonstrated for the microscopic analysis of granules from upflow-anaerobic-sludge-blanket (UASB) reactors. Granules were first fixed with formaldehyde and embedded in paraffin, before being sectioned by a microtome to a thickness of 3-5 μm. The section was then mounted on a glass slide for microscopic examination using phase contrast and epi-fluorescent excitation. Using this simple method, the microstructure of the granules and the distribution of methanogenic bacteria could be analyzed. Over 100 granules from UASB reactors treating sucrose wastewaters were examined. Microscopic photos showed that the granules had a surface layer comprised of hydrogen-consuming methanogenic cocci and bacilli, and a loosely packed interior, mainly comprised of acetotrophic Methanothrix. This simple method provides engineers with a practical tool for studying the microstructure of the granules, the mechanisms of their formation, and kinetic modeling.en_US
dc.languageengen_US
dc.publisherAmerican Society of Civil Engineers. The Journal's web site is located at http://www.pubs.asce.org/journals/ee.htmlen_US
dc.relation.ispartofJournal of Environmental Engineeringen_US
dc.titleHistological analysis of microstructure of UASB granulesen_US
dc.typeArticleen_US
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_US
dc.identifier.authorityFang, HHP=rp00115en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0028500205en_US
dc.identifier.volume120en_US
dc.identifier.issue5en_US
dc.identifier.spage1322en_US
dc.identifier.epage1326en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChui, HK=7006642070en_US
dc.identifier.scopusauthoridFang, HHP=7402542625en_US

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