Article: Dose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters
| Title | Dose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters | ||||||
|---|---|---|---|---|---|---|---|
| Authors | You, S2 So, KF1 Li, Y3 Yang, A2 Zhu, T3 Liu, Z2 | ||||||
| Keywords | Hamster N-Methyl-D-Aspartate Receptor Neuronal Survival Nitric Oxide Synthase Optic Nerve Transection Retinal Ganglion Cells | ||||||
| Issue Date | 2012 | ||||||
| Publisher | Neural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com | ||||||
| Citation | Neural Regeneration Research, 2012, v. 7 n. 10, p. 725-730 [How to Cite?] DOI: http://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001 | ||||||
| Abstract | This study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect. | ||||||
| ISSN | 1673-5374 2011 Impact Factor: 0.216 2011 SCImago Journal Rankings: 0.029 | ||||||
| DOI | http://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001 | ||||||
| ISI Accession Number ID | WOS:000303778200001
Funding Information: This research work was supported by research grants from Chinese National Key Project for Basic Research, No. 2011CB504402 and the National Natural Science Foundation of China, No. 30901649 and 30872829. | ||||||
| References | References in Scopus |
| dc.contributor.author | You, S | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | So, KF | ||||||
| dc.contributor.author | Li, Y | ||||||
| dc.contributor.author | Yang, A | ||||||
| dc.contributor.author | Zhu, T | ||||||
| dc.contributor.author | Liu, Z | ||||||
| dc.date.accessioned | 2012-06-26T05:58:44Z | ||||||
| dc.date.available | 2012-06-26T05:58:44Z | ||||||
| dc.date.issued | 2012 | ||||||
| dc.description.abstract | This study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Neural Regeneration Research, 2012, v. 7 n. 10, p. 725-730 [How to Cite?] DOI: http://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001 | ||||||
| dc.identifier.epage | 730 | ||||||
| dc.identifier.hkuros | 207145 | ||||||
| dc.identifier.isi | WOS:000303778200001
Funding Information: This research work was supported by research grants from Chinese National Key Project for Basic Research, No. 2011CB504402 and the National Natural Science Foundation of China, No. 30901649 and 30872829. | ||||||
| dc.identifier.issn | 1673-5374 2011 Impact Factor: 0.216 2011 SCImago Journal Rankings: 0.029 | ||||||
| dc.identifier.issue | 10 | ||||||
| dc.identifier.scopus | eid_2-s2.0-84861320764 | ||||||
| dc.identifier.spage | 725 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/149790 | ||||||
| dc.identifier.volume | 7 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Neural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com | ||||||
| dc.publisher.place | China | ||||||
| dc.relation.ispartof | Neural Regeneration Research | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject | Hamster | ||||||
| dc.subject | N-Methyl-D-Aspartate Receptor | ||||||
| dc.subject | Neuronal Survival | ||||||
| dc.subject | Nitric Oxide Synthase | ||||||
| dc.subject | Optic Nerve Transection | ||||||
| dc.subject | Retinal Ganglion Cells | ||||||
| dc.title | Dose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- The Fourth Military Medical University
- General Hospital of People's Liberation Army