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Article: Dose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters

TitleDose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters
Authors
KeywordsHamster
N-Methyl-D-Aspartate Receptor
Neuronal Survival
Nitric Oxide Synthase
Optic Nerve Transection
Retinal Ganglion Cells
Issue Date2012
PublisherMedknow Publications and Media Pvt. Ltd. The Journal's web site is located at http://www.nrronline.org/
Citation
Neural Regeneration Research, 2012, v. 7 n. 10, p. 725-730 How to Cite?
AbstractThis study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect.
Persistent Identifierhttp://hdl.handle.net/10722/149790
ISSN
2014 Impact Factor: 0.220
2014 SCImago Journal Rankings: 0.148
ISI Accession Number ID
Funding AgencyGrant Number
Chinese National Key Project for Basic Researc2011CB504402
National Natural Science Foundation of China30901649
30872829
Funding Information:

This research work was supported by research grants from Chinese National Key Project for Basic Research, No. 2011CB504402 and the National Natural Science Foundation of China, No. 30901649 and 30872829.

References

 

DC FieldValueLanguage
dc.contributor.authorYou, Sen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorYang, Aen_US
dc.contributor.authorZhu, Ten_US
dc.contributor.authorLiu, Zen_US
dc.date.accessioned2012-06-26T05:58:44Z-
dc.date.available2012-06-26T05:58:44Z-
dc.date.issued2012en_US
dc.identifier.citationNeural Regeneration Research, 2012, v. 7 n. 10, p. 725-730en_US
dc.identifier.issn1673-5374en_US
dc.identifier.urihttp://hdl.handle.net/10722/149790-
dc.description.abstractThis study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect.en_US
dc.languageengen_US
dc.publisherMedknow Publications and Media Pvt. Ltd. The Journal's web site is located at http://www.nrronline.org/en_US
dc.relation.ispartofNeural Regeneration Researchen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectHamsteren_US
dc.subjectN-Methyl-D-Aspartate Receptoren_US
dc.subjectNeuronal Survivalen_US
dc.subjectNitric Oxide Synthaseen_US
dc.subjectOptic Nerve Transectionen_US
dc.subjectRetinal Ganglion Cellsen_US
dc.titleDose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamstersen_US
dc.typeArticleen_US
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_US
dc.identifier.authoritySo, KF=rp00329en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.3969/j.issn.1673-5374.2012.10.001en_US
dc.identifier.scopuseid_2-s2.0-84861320764en_US
dc.identifier.hkuros207145-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861320764&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume7en_US
dc.identifier.issue10en_US
dc.identifier.spage725en_US
dc.identifier.epage730en_US
dc.identifier.isiWOS:000303778200001-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridYou, S=8226423300en_US
dc.identifier.scopusauthoridSo, KF=34668391300en_US
dc.identifier.scopusauthoridLi, Y=7502078402en_US
dc.identifier.scopusauthoridYang, A=55223967800en_US
dc.identifier.scopusauthoridZhu, T=55224331000en_US
dc.identifier.scopusauthoridLiu, Z=7406677201en_US

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