File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Dose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters
  • Basic View
  • Metadata View
  • XML View
TitleDose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters
 
AuthorsYou, S2
So, KF1
Li, Y3
Yang, A2
Zhu, T3
Liu, Z2
 
KeywordsHamster
N-Methyl-D-Aspartate Receptor
Neuronal Survival
Nitric Oxide Synthase
Optic Nerve Transection
Retinal Ganglion Cells
 
Issue Date2012
 
PublisherNeural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com
 
CitationNeural Regeneration Research, 2012, v. 7 n. 10, p. 725-730 [How to Cite?]
DOI: http://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001
 
AbstractThis study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect.
 
ISSN1673-5374
2013 Impact Factor: 0.234
2013 SCImago Journal Rankings: 0.158
 
DOIhttp://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001
 
ISI Accession Number IDWOS:000303778200001
Funding AgencyGrant Number
Chinese National Key Project for Basic Researc2011CB504402
National Natural Science Foundation of China30901649
30872829
Funding Information:

This research work was supported by research grants from Chinese National Key Project for Basic Research, No. 2011CB504402 and the National Natural Science Foundation of China, No. 30901649 and 30872829.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYou, S
 
dc.contributor.authorSo, KF
 
dc.contributor.authorLi, Y
 
dc.contributor.authorYang, A
 
dc.contributor.authorZhu, T
 
dc.contributor.authorLiu, Z
 
dc.date.accessioned2012-06-26T05:58:44Z
 
dc.date.available2012-06-26T05:58:44Z
 
dc.date.issued2012
 
dc.description.abstractThis study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationNeural Regeneration Research, 2012, v. 7 n. 10, p. 725-730 [How to Cite?]
DOI: http://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001
 
dc.identifier.doihttp://dx.doi.org/10.3969/j.issn.1673-5374.2012.10.001
 
dc.identifier.epage730
 
dc.identifier.hkuros207145
 
dc.identifier.isiWOS:000303778200001
Funding AgencyGrant Number
Chinese National Key Project for Basic Researc2011CB504402
National Natural Science Foundation of China30901649
30872829
Funding Information:

This research work was supported by research grants from Chinese National Key Project for Basic Research, No. 2011CB504402 and the National Natural Science Foundation of China, No. 30901649 and 30872829.

 
dc.identifier.issn1673-5374
2013 Impact Factor: 0.234
2013 SCImago Journal Rankings: 0.158
 
dc.identifier.issue10
 
dc.identifier.scopuseid_2-s2.0-84861320764
 
dc.identifier.spage725
 
dc.identifier.urihttp://hdl.handle.net/10722/149790
 
dc.identifier.volume7
 
dc.languageeng
 
dc.publisherNeural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com
 
dc.publisher.placeChina
 
dc.relation.ispartofNeural Regeneration Research
 
dc.relation.referencesReferences in Scopus
 
dc.subjectHamster
 
dc.subjectN-Methyl-D-Aspartate Receptor
 
dc.subjectNeuronal Survival
 
dc.subjectNitric Oxide Synthase
 
dc.subjectOptic Nerve Transection
 
dc.subjectRetinal Ganglion Cells
 
dc.titleDose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>You, S</contributor.author>
<contributor.author>So, KF</contributor.author>
<contributor.author>Li, Y</contributor.author>
<contributor.author>Yang, A</contributor.author>
<contributor.author>Zhu, T</contributor.author>
<contributor.author>Liu, Z</contributor.author>
<date.accessioned>2012-06-26T05:58:44Z</date.accessioned>
<date.available>2012-06-26T05:58:44Z</date.available>
<date.issued>2012</date.issued>
<identifier.citation>Neural Regeneration Research, 2012, v. 7 n. 10, p. 725-730</identifier.citation>
<identifier.issn>1673-5374</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/149790</identifier.uri>
<description.abstract>This study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect.</description.abstract>
<language>eng</language>
<publisher>Neural Regeneration Research. The Journal&apos;s web site is located at http://www.sjzsyj.com</publisher>
<relation.ispartof>Neural Regeneration Research</relation.ispartof>
<subject>Hamster</subject>
<subject>N-Methyl-D-Aspartate Receptor</subject>
<subject>Neuronal Survival</subject>
<subject>Nitric Oxide Synthase</subject>
<subject>Optic Nerve Transection</subject>
<subject>Retinal Ganglion Cells</subject>
<title>Dose-dependent and combined effects of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters</title>
<type>Article</type>
<description.nature>link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.3969/j.issn.1673-5374.2012.10.001</identifier.doi>
<identifier.scopus>eid_2-s2.0-84861320764</identifier.scopus>
<identifier.hkuros>207145</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-84861320764&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>7</identifier.volume>
<identifier.issue>10</identifier.issue>
<identifier.spage>725</identifier.spage>
<identifier.epage>730</identifier.epage>
<identifier.isi>WOS:000303778200001</identifier.isi>
<publisher.place>China</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The Fourth Military Medical University
  3. General Hospital of People's Liberation Army