Article: Cigarette smoking accelerated brain aging and induced pre-Alzheimer-like neuropathology in rats
| Title | Cigarette smoking accelerated brain aging and induced pre-Alzheimer-like neuropathology in rats | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Authors | Ho, YS3 Yang, X2 Yeung, SC1 Chiu, K3 Lau, CF3 Tsang, AWT3 Mak, JCW1 3 Chang, RCC3 | ||||||||
| Keywords | Alzheimer disease Animal experiment Cigarette smoking Cytoskeleton Dentate gyrus | ||||||||
| Issue Date | 2012 | ||||||||
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||||
| Citation | PLoS ONE, 2012, v. 7 n. 5, article no. e36752 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0036752 | ||||||||
| Abstract | Cigarette smoking has been proposed as a major risk factor for aging-related pathological changes and Alzheimer's disease (AD). To date, little is known for how smoking can predispose our brains to dementia or cognitive impairment. This study aimed to investigate the cigarette smoke-induced pathological changes in brains. Male Sprague-Dawley (SD) rats were exposed to either sham air or 4% cigarette smoke 1 hour per day for 8 weeks in a ventilated smoking chamber to mimic the situation of chronic passive smoking. We found that the levels of oxidative stress were significantly increased in the hippocampus of the smoking group. Smoking also affected the synapse through reducing the expression of pre-synaptic proteins including synaptophysin and synapsin-1, while there were no changes in the expression of postsynaptic protein PSD95. Decreased levels of acetylated-tubulin and increased levels of phosphorylated-tau at 231, 205 and 404 epitopes were also observed in the hippocampus of the smoking rats. These results suggested that axonal transport machinery might be impaired, and the stability of cytoskeleton might be affected by smoking. Moreover, smoking affected amyloid precursor protein (APP) processing by increasing the production of sAPPbeta and accumulation of beta-amyloid peptide in the CA3 and dentate gyrus region. In summary, our data suggested that chronic cigarette smoking could induce synaptic changes and other neuropathological alterations. These changes might serve as evidence of early phases of neurodegeneration and may explain why smoking can predispose brains to AD and dementia. | ||||||||
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||||
| DOI | http://dx.doi.org/10.1371/journal.pone.0036752 | ||||||||
| ISI Accession Number ID | WOS:000305338200040
Funding Information: Sources of funding support include: HKU (University of Hong Kong) Alzheimer's Disease Research Network under Strategic Research Theme on Healthy Aging, GRF 761609M & NSFC/RGC70707M, Seed Funding for Basic Science Research (201011159058) to RCCC, Small Grant Research (201007176112) to YSH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||||
| PubMed Central ID | PMC3350465 | ||||||||
| References | References in Scopus | ||||||||
| Grants | Elucidating pathophysiological mechanisms of synaptic and axonal degeneration in depression and Alzheimer's disease |
| dc.contributor.author | Ho, YS | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Yang, X | ||||||||
| dc.contributor.author | Yeung, SC | ||||||||
| dc.contributor.author | Chiu, K | ||||||||
| dc.contributor.author | Lau, CF | ||||||||
| dc.contributor.author | Tsang, AWT | ||||||||
| dc.contributor.author | Mak, JCW | ||||||||
| dc.contributor.author | Chang, RCC | ||||||||
| dc.date.accessioned | 2012-06-26T05:58:42Z | ||||||||
| dc.date.available | 2012-06-26T05:58:42Z | ||||||||
| dc.date.issued | 2012 | ||||||||
| dc.description.abstract | Cigarette smoking has been proposed as a major risk factor for aging-related pathological changes and Alzheimer's disease (AD). To date, little is known for how smoking can predispose our brains to dementia or cognitive impairment. This study aimed to investigate the cigarette smoke-induced pathological changes in brains. Male Sprague-Dawley (SD) rats were exposed to either sham air or 4% cigarette smoke 1 hour per day for 8 weeks in a ventilated smoking chamber to mimic the situation of chronic passive smoking. We found that the levels of oxidative stress were significantly increased in the hippocampus of the smoking group. Smoking also affected the synapse through reducing the expression of pre-synaptic proteins including synaptophysin and synapsin-1, while there were no changes in the expression of postsynaptic protein PSD95. Decreased levels of acetylated-tubulin and increased levels of phosphorylated-tau at 231, 205 and 404 epitopes were also observed in the hippocampus of the smoking rats. These results suggested that axonal transport machinery might be impaired, and the stability of cytoskeleton might be affected by smoking. Moreover, smoking affected amyloid precursor protein (APP) processing by increasing the production of sAPPbeta and accumulation of beta-amyloid peptide in the CA3 and dentate gyrus region. In summary, our data suggested that chronic cigarette smoking could induce synaptic changes and other neuropathological alterations. These changes might serve as evidence of early phases of neurodegeneration and may explain why smoking can predispose brains to AD and dementia. | ||||||||
| dc.description.grant | Elucidating pathophysiological mechanisms of synaptic and axonal degeneration in depression and Alzheimer's disease | ||||||||
| dc.description.grantcode | 104422 | ||||||||
| dc.description.nature | published_or_final_version | ||||||||
| dc.identifier.citation | PLoS ONE, 2012, v. 7 n. 5, article no. e36752 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0036752 | ||||||||
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0036752 | ||||||||
| dc.identifier.hkuros | 200046 | ||||||||
| dc.identifier.isi | WOS:000305338200040
Funding Information: Sources of funding support include: HKU (University of Hong Kong) Alzheimer's Disease Research Network under Strategic Research Theme on Healthy Aging, GRF 761609M & NSFC/RGC70707M, Seed Funding for Basic Science Research (201011159058) to RCCC, Small Grant Research (201007176112) to YSH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||||
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||||
| dc.identifier.issue | 5, article no. e36752 | ||||||||
| dc.identifier.pmcid | PMC3350465 | ||||||||
| dc.identifier.pmid | 22606286 | ||||||||
| dc.identifier.scopus | eid_2-s2.0-84861003588 | ||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/149789 | ||||||||
| dc.identifier.volume | 7 | ||||||||
| dc.language | eng | ||||||||
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||||
| dc.publisher.place | United States | ||||||||
| dc.relation.ispartof | PLoS ONE | ||||||||
| dc.relation.references | References in Scopus | ||||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||||
| dc.subject | Alzheimer disease | ||||||||
| dc.subject | Animal experiment | ||||||||
| dc.subject | Cigarette smoking | ||||||||
| dc.subject | Cytoskeleton | ||||||||
| dc.subject | Dentate gyrus | ||||||||
| dc.title | Cigarette smoking accelerated brain aging and induced pre-Alzheimer-like neuropathology in rats | ||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- Shenzhen Centre of Disease Control and Prevention
- The University of Hong Kong