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Article: Activation of phospholipase-Cγ and protein kinase C signal pathways helps the survival of spinal motoneurons injured by root avulsion

TitleActivation of phospholipase-Cγ and protein kinase C signal pathways helps the survival of spinal motoneurons injured by root avulsion
Authors
KeywordsNeuronal Nitric Oxide Synthase
Pkc
Plcγ
Root Avulsion
Spinal Motoneuron
Issue Date2012
Citation
Journal Of Neurochemistry, 2012, v. 121 n. 3, p. 362-372 How to Cite?
AbstractThe signaling transduction processes involved in avulsion-induced motoneuron (MN) death have not been elucidated. Using the brachial plexus root avulsion rat model, we showed that avulsion-activated phosphorylation of phospholipase-Cγ (PLCγ) and protein kinase C (PKC) occurred in injured spinal MNs within 72 h of injury. Moreover, some MNs positive for PLCγ and PKC are also positive for avulsion-induced neuronal nitric oxide synthase (nNOS). Inhibition of PLCγ/PKC signal pathway, either with PLCγ inhibitor, 1-[6-((17β-3-methoxyestra-1,3,5(10)-trien-17-yl) amino)hexyl]-1H-pyrrole-2,5-dione, or with PLCγ siRNA augmented avulsion-induced MN death. 1-[6-((17β-3-Methoxyestra-1,3,5(10)-trien-17-yl) amino)hexyl]-1H-pyrrole-2,5-dione also inhibited PKC phosphorylation and exacerbated avulsion-induced reductions in the nNOS protein level in injured spinal segments. Moreover, activation of PLCγ/PKC signal pathway with PKC activator, phorbol-12-myristate-13-acetate, decreased avulsion-induced MN death. The temporal profile of PLCγ/PKC signaling appears to be crucial for the survival of spinal MNs after root avulsion. Our data suggest that PLCγ mediates, while PKC and nNOS are associated with, the avulsion-induced MN death in brachial plexus root avulsion. © 2012 International Society for Neurochemistry.
Persistent Identifierhttp://hdl.handle.net/10722/149787
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021
ISI Accession Number ID
Funding AgencyGrant Number
National Science Foundation Council of China31171290
81070995
Guangdong Science Foundations2010B031600037
Funding Information:

This work was supported by research grants from the National Science Foundation Council of China (31171290; 81070995) and Guangdong Science Foundations (2010B031600037). Grateful acknowledgement is made to Prof. Peter J. Little who helped modify and editing the manuscript in English.

References

 

DC FieldValueLanguage
dc.contributor.authorZhao, XCen_US
dc.contributor.authorWang, LLen_US
dc.contributor.authorWang, YQen_US
dc.contributor.authorSong, FHen_US
dc.contributor.authorLi, YQen_US
dc.contributor.authorFu, Ren_US
dc.contributor.authorZheng, WHen_US
dc.contributor.authorWu, Wen_US
dc.contributor.authorZhou, LHen_US
dc.date.accessioned2012-06-26T05:58:39Z-
dc.date.available2012-06-26T05:58:39Z-
dc.date.issued2012en_US
dc.identifier.citationJournal Of Neurochemistry, 2012, v. 121 n. 3, p. 362-372en_US
dc.identifier.issn0022-3042en_US
dc.identifier.urihttp://hdl.handle.net/10722/149787-
dc.description.abstractThe signaling transduction processes involved in avulsion-induced motoneuron (MN) death have not been elucidated. Using the brachial plexus root avulsion rat model, we showed that avulsion-activated phosphorylation of phospholipase-Cγ (PLCγ) and protein kinase C (PKC) occurred in injured spinal MNs within 72 h of injury. Moreover, some MNs positive for PLCγ and PKC are also positive for avulsion-induced neuronal nitric oxide synthase (nNOS). Inhibition of PLCγ/PKC signal pathway, either with PLCγ inhibitor, 1-[6-((17β-3-methoxyestra-1,3,5(10)-trien-17-yl) amino)hexyl]-1H-pyrrole-2,5-dione, or with PLCγ siRNA augmented avulsion-induced MN death. 1-[6-((17β-3-Methoxyestra-1,3,5(10)-trien-17-yl) amino)hexyl]-1H-pyrrole-2,5-dione also inhibited PKC phosphorylation and exacerbated avulsion-induced reductions in the nNOS protein level in injured spinal segments. Moreover, activation of PLCγ/PKC signal pathway with PKC activator, phorbol-12-myristate-13-acetate, decreased avulsion-induced MN death. The temporal profile of PLCγ/PKC signaling appears to be crucial for the survival of spinal MNs after root avulsion. Our data suggest that PLCγ mediates, while PKC and nNOS are associated with, the avulsion-induced MN death in brachial plexus root avulsion. © 2012 International Society for Neurochemistry.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Neurochemistryen_US
dc.subjectNeuronal Nitric Oxide Synthaseen_US
dc.subjectPkcen_US
dc.subjectPlcγen_US
dc.subjectRoot Avulsionen_US
dc.subjectSpinal Motoneuronen_US
dc.titleActivation of phospholipase-Cγ and protein kinase C signal pathways helps the survival of spinal motoneurons injured by root avulsionen_US
dc.typeArticleen_US
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, W=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1471-4159.2012.07696.xen_US
dc.identifier.pmid22339041-
dc.identifier.scopuseid_2-s2.0-84862789966-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84859583289&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume121en_US
dc.identifier.issue3en_US
dc.identifier.spage362en_US
dc.identifier.epage372en_US
dc.identifier.isiWOS:000302615700005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridZhao, XC=55074654900en_US
dc.identifier.scopusauthoridWang, LL=55080151000en_US
dc.identifier.scopusauthoridWang, YQ=55076620500en_US
dc.identifier.scopusauthoridSong, FH=55075903200en_US
dc.identifier.scopusauthoridLi, YQ=55075198200en_US
dc.identifier.scopusauthoridFu, R=47761080500en_US
dc.identifier.scopusauthoridZheng, WH=55079029800en_US
dc.identifier.scopusauthoridWu, W=7407081122en_US
dc.identifier.scopusauthoridZhou, LH=7404125592en_US

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