Article: Optimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion
| Title | Optimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Su, H2 Wu, Y3 Yuan, Q2 Guo, J1 Zhang, W2 5 Wu, W2 4 | ||||||
| Issue Date | 2011 | ||||||
| Citation | Cell Transplantation, 2011, v. 20 n. 2, p. 167-176 [How to Cite?] DOI: http://dx.doi.org/10.3727/096368910X522090 | ||||||
| Abstract | Root avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF. © 2011 Cognizant Comm. Corp. | ||||||
| ISSN | 0963-6897 2011 SCImago Journal Rankings: 0.260 | ||||||
| DOI | http://dx.doi.org/10.3727/096368910X522090 | ||||||
| ISI Accession Number ID | WOS:000289322000003
Funding Information: This study was supported by HKU Spinal Cord Injury Foundation and National key basic research support foundation (973 project: 2011CB504402). | ||||||
| References | References in Scopus |
| dc.contributor.author | Su, H | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Wu, Y | ||||||
| dc.contributor.author | Yuan, Q | ||||||
| dc.contributor.author | Guo, J | ||||||
| dc.contributor.author | Zhang, W | ||||||
| dc.contributor.author | Wu, W | ||||||
| dc.date.accessioned | 2012-06-26T05:58:11Z | ||||||
| dc.date.available | 2012-06-26T05:58:11Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Root avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF. © 2011 Cognizant Comm. Corp. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Cell Transplantation, 2011, v. 20 n. 2, p. 167-176 [How to Cite?] DOI: http://dx.doi.org/10.3727/096368910X522090 | ||||||
| dc.identifier.citeulike | 9121753 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.3727/096368910X522090 | ||||||
| dc.identifier.epage | 176 | ||||||
| dc.identifier.isi | WOS:000289322000003
Funding Information: This study was supported by HKU Spinal Cord Injury Foundation and National key basic research support foundation (973 project: 2011CB504402). | ||||||
| dc.identifier.issn | 0963-6897 2011 SCImago Journal Rankings: 0.260 | ||||||
| dc.identifier.issue | 2 | ||||||
| dc.identifier.pmid | 20719091 | ||||||
| dc.identifier.scopus | eid_2-s2.0-79955395422 | ||||||
| dc.identifier.spage | 167 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/149762 | ||||||
| dc.identifier.volume | 20 | ||||||
| dc.language | eng | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Cell Transplantation | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Animals | ||||||
| dc.subject.mesh | Anterior Horn Cells - Pathology | ||||||
| dc.subject.mesh | Brain-Derived Neurotrophic Factor - Metabolism | ||||||
| dc.subject.mesh | Cell Differentiation | ||||||
| dc.subject.mesh | Cells, Cultured | ||||||
| dc.subject.mesh | Choline O-Acetyltransferase - Metabolism | ||||||
| dc.subject.mesh | Female | ||||||
| dc.subject.mesh | Glial Cell Line-Derived Neurotrophic Factor - Metabolism | ||||||
| dc.subject.mesh | Neural Stem Cells - Transplantation | ||||||
| dc.subject.mesh | Neurons - Cytology - Enzymology | ||||||
| dc.subject.mesh | Radiculopathy - Complications - Pathology - Therapy | ||||||
| dc.subject.mesh | Rats | ||||||
| dc.subject.mesh | Rats, Sprague-Dawley | ||||||
| dc.subject.mesh | Spinal Cord Injuries - Complications - Therapy | ||||||
| dc.subject.mesh | Stem Cell Transplantation | ||||||
| dc.subject.mesh | Time Factors | ||||||
| dc.title | Optimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion | ||||||
| dc.type | Article |
Author Affiliations
- Southern Medical University
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- UCL Institute of Child Health
- Jinan University
- First Affiliated Hospital of Fujian Medical University