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Article: Optimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion

TitleOptimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion
Authors
Issue Date2011
Citation
Cell Transplantation, 2011, v. 20 n. 2, p. 167-176 How to Cite?
Abstract
Root avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF. © 2011 Cognizant Comm. Corp.
Persistent Identifierhttp://hdl.handle.net/10722/149762
ISSN
2013 Impact Factor: 3.570
2013 SCImago Journal Rankings: 1.303
ISI Accession Number ID
Funding AgencyGrant Number
HKU Spinal Cord Injury Foundation
National key basic research support foundation2011CB504402
Funding Information:

This study was supported by HKU Spinal Cord Injury Foundation and National key basic research support foundation (973 project: 2011CB504402).

References

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Southern Medical University
  3. UCL Institute of Child Health
  4. Jinan University
  5. First Affiliated Hospital of Fujian Medical University
DC FieldValueLanguage
dc.contributor.authorSu, Hen_US
dc.contributor.authorWu, Yen_US
dc.contributor.authorYuan, Qen_US
dc.contributor.authorGuo, Jen_US
dc.contributor.authorZhang, Wen_US
dc.contributor.authorWu, Wen_US
dc.date.accessioned2012-06-26T05:58:11Z-
dc.date.available2012-06-26T05:58:11Z-
dc.date.issued2011en_US
dc.identifier.citationCell Transplantation, 2011, v. 20 n. 2, p. 167-176en_US
dc.identifier.issn0963-6897en_US
dc.identifier.urihttp://hdl.handle.net/10722/149762-
dc.description.abstractRoot avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF. © 2011 Cognizant Comm. Corp.en_US
dc.languageengen_US
dc.relation.ispartofCell Transplantationen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnterior Horn Cells - Pathologyen_US
dc.subject.meshBrain-Derived Neurotrophic Factor - Metabolismen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCholine O-Acetyltransferase - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshGlial Cell Line-Derived Neurotrophic Factor - Metabolismen_US
dc.subject.meshNeural Stem Cells - Transplantationen_US
dc.subject.meshNeurons - Cytology - Enzymologyen_US
dc.subject.meshRadiculopathy - Complications - Pathology - Therapyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSpinal Cord Injuries - Complications - Therapyen_US
dc.subject.meshStem Cell Transplantationen_US
dc.subject.meshTime Factorsen_US
dc.titleOptimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsionen_US
dc.typeArticleen_US
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, W=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3727/096368910X522090en_US
dc.identifier.pmid20719091en_US
dc.identifier.scopuseid_2-s2.0-79955395422en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955395422&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue2en_US
dc.identifier.spage167en_US
dc.identifier.epage176en_US
dc.identifier.isiWOS:000289322000003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSu, H=16317750200en_US
dc.identifier.scopusauthoridWu, Y=36130811800en_US
dc.identifier.scopusauthoridYuan, Q=7202814773en_US
dc.identifier.scopusauthoridGuo, J=50361236400en_US
dc.identifier.scopusauthoridZhang, W=9044147100en_US
dc.identifier.scopusauthoridWu, W=7407081122en_US
dc.identifier.citeulike9121753-

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