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Article: Optimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion
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TitleOptimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion
 
AuthorsSu, H1
Wu, Y3
Yuan, Q1
Guo, J2
Zhang, W1 5
Wu, W1 4
 
Issue Date2011
 
CitationCell Transplantation, 2011, v. 20 n. 2, p. 167-176 [How to Cite?]
DOI: http://dx.doi.org/10.3727/096368910X522090
 
AbstractRoot avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF. © 2011 Cognizant Comm. Corp.
 
ISSN0963-6897
2012 Impact Factor: 4.422
2012 SCImago Journal Rankings: 1.312
 
DOIhttp://dx.doi.org/10.3727/096368910X522090
 
ISI Accession Number IDWOS:000289322000003
Funding AgencyGrant Number
HKU Spinal Cord Injury Foundation
National key basic research support foundation2011CB504402
Funding Information:

This study was supported by HKU Spinal Cord Injury Foundation and National key basic research support foundation (973 project: 2011CB504402).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSu, H
 
dc.contributor.authorWu, Y
 
dc.contributor.authorYuan, Q
 
dc.contributor.authorGuo, J
 
dc.contributor.authorZhang, W
 
dc.contributor.authorWu, W
 
dc.date.accessioned2012-06-26T05:58:11Z
 
dc.date.available2012-06-26T05:58:11Z
 
dc.date.issued2011
 
dc.description.abstractRoot avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF. © 2011 Cognizant Comm. Corp.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCell Transplantation, 2011, v. 20 n. 2, p. 167-176 [How to Cite?]
DOI: http://dx.doi.org/10.3727/096368910X522090
 
dc.identifier.citeulike9121753
 
dc.identifier.doihttp://dx.doi.org/10.3727/096368910X522090
 
dc.identifier.epage176
 
dc.identifier.isiWOS:000289322000003
Funding AgencyGrant Number
HKU Spinal Cord Injury Foundation
National key basic research support foundation2011CB504402
Funding Information:

This study was supported by HKU Spinal Cord Injury Foundation and National key basic research support foundation (973 project: 2011CB504402).

 
dc.identifier.issn0963-6897
2012 Impact Factor: 4.422
2012 SCImago Journal Rankings: 1.312
 
dc.identifier.issue2
 
dc.identifier.pmid20719091
 
dc.identifier.scopuseid_2-s2.0-79955395422
 
dc.identifier.spage167
 
dc.identifier.urihttp://hdl.handle.net/10722/149762
 
dc.identifier.volume20
 
dc.languageeng
 
dc.publisher.placeUnited States
 
dc.relation.ispartofCell Transplantation
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAnterior Horn Cells - Pathology
 
dc.subject.meshBrain-Derived Neurotrophic Factor - Metabolism
 
dc.subject.meshCell Differentiation
 
dc.subject.meshCells, Cultured
 
dc.subject.meshCholine O-Acetyltransferase - Metabolism
 
dc.subject.meshFemale
 
dc.subject.meshGlial Cell Line-Derived Neurotrophic Factor - Metabolism
 
dc.subject.meshNeural Stem Cells - Transplantation
 
dc.subject.meshNeurons - Cytology - Enzymology
 
dc.subject.meshRadiculopathy - Complications - Pathology - Therapy
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshSpinal Cord Injuries - Complications - Therapy
 
dc.subject.meshStem Cell Transplantation
 
dc.subject.meshTime Factors
 
dc.titleOptimal time point for neuronal generation of transplanted neural progenitor cells in injured Spinal cord following root avulsion
 
dc.typeArticle
 
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<contributor.author>Zhang, W</contributor.author>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Southern Medical University
  3. UCL Institute of Child Health
  4. Jinan University
  5. First Affiliated Hospital of Fujian Medical University